Optimising cell aggregate expansion in a perfused hollow fibre bioreactor via mathematical modelling.

The need for efficient and controlled expansion of cell populations is paramount in tissue engineering. Hollow fibre bioreactors (HFBs) have the potential to meet this need, but only with improved understanding of how operating conditions and cell seeding strategy affect cell proliferation in the bi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lloyd A C Chapman, Rebecca J Shipley, Jonathan P Whiteley, Marianne J Ellis, Helen M Byrne, Sarah L Waters
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e8d7f704587d42848b1366cb2516c3ab
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e8d7f704587d42848b1366cb2516c3ab
record_format	dspace
spelling	oai:doaj.org-article:e8d7f704587d42848b1366cb2516c3ab2021-11-25T06:03:11ZOptimising cell aggregate expansion in a perfused hollow fibre bioreactor via mathematical modelling.1932-620310.1371/journal.pone.0105813https://doaj.org/article/e8d7f704587d42848b1366cb2516c3ab2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25157635/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The need for efficient and controlled expansion of cell populations is paramount in tissue engineering. Hollow fibre bioreactors (HFBs) have the potential to meet this need, but only with improved understanding of how operating conditions and cell seeding strategy affect cell proliferation in the bioreactor. This study is designed to assess the effects of two key operating parameters (the flow rate of culture medium into the fibre lumen and the fluid pressure imposed at the lumen outlet), together with the cell seeding distribution, on cell population growth in a single-fibre HFB. This is achieved using mathematical modelling and numerical methods to simulate the growth of cell aggregates along the outer surface of the fibre in response to the local oxygen concentration and fluid shear stress. The oxygen delivery to the cell aggregates and the fluid shear stress increase as the flow rate and pressure imposed at the lumen outlet are increased. Although the increased oxygen delivery promotes growth, the higher fluid shear stress can lead to cell death. For a given cell type and initial aggregate distribution, the operating parameters that give the most rapid overall growth can be identified from simulations. For example, when aggregates of rat cardiomyocytes that can tolerate shear stresses of up to 0:05 Pa are evenly distributed along the fibre, the inlet flow rate and outlet pressure that maximise the overall growth rate are predicted to be in the ranges 2.75 x 10(-5) m(2) s(-1) to 3 x 10(-5) m(2) s(-1) (equivalent to 2.07 ml min(-1) to 2.26 ml min(-1)) and 1.077 x 10(5) Pa to 1.083 x 10(5) Pa (or 15.6 psi to 15.7 psi) respectively. The combined effects of the seeding distribution and flow on the growth are also investigated and the optimal conditions for growth found to depend on the shear tolerance and oxygen demands of the cells.Lloyd A C ChapmanRebecca J ShipleyJonathan P WhiteleyMarianne J EllisHelen M ByrneSarah L WatersPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e105813 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lloyd A C Chapman Rebecca J Shipley Jonathan P Whiteley Marianne J Ellis Helen M Byrne Sarah L Waters Optimising cell aggregate expansion in a perfused hollow fibre bioreactor via mathematical modelling.
description	The need for efficient and controlled expansion of cell populations is paramount in tissue engineering. Hollow fibre bioreactors (HFBs) have the potential to meet this need, but only with improved understanding of how operating conditions and cell seeding strategy affect cell proliferation in the bioreactor. This study is designed to assess the effects of two key operating parameters (the flow rate of culture medium into the fibre lumen and the fluid pressure imposed at the lumen outlet), together with the cell seeding distribution, on cell population growth in a single-fibre HFB. This is achieved using mathematical modelling and numerical methods to simulate the growth of cell aggregates along the outer surface of the fibre in response to the local oxygen concentration and fluid shear stress. The oxygen delivery to the cell aggregates and the fluid shear stress increase as the flow rate and pressure imposed at the lumen outlet are increased. Although the increased oxygen delivery promotes growth, the higher fluid shear stress can lead to cell death. For a given cell type and initial aggregate distribution, the operating parameters that give the most rapid overall growth can be identified from simulations. For example, when aggregates of rat cardiomyocytes that can tolerate shear stresses of up to 0:05 Pa are evenly distributed along the fibre, the inlet flow rate and outlet pressure that maximise the overall growth rate are predicted to be in the ranges 2.75 x 10(-5) m(2) s(-1) to 3 x 10(-5) m(2) s(-1) (equivalent to 2.07 ml min(-1) to 2.26 ml min(-1)) and 1.077 x 10(5) Pa to 1.083 x 10(5) Pa (or 15.6 psi to 15.7 psi) respectively. The combined effects of the seeding distribution and flow on the growth are also investigated and the optimal conditions for growth found to depend on the shear tolerance and oxygen demands of the cells.
format	article
author	Lloyd A C Chapman Rebecca J Shipley Jonathan P Whiteley Marianne J Ellis Helen M Byrne Sarah L Waters
author_facet	Lloyd A C Chapman Rebecca J Shipley Jonathan P Whiteley Marianne J Ellis Helen M Byrne Sarah L Waters
author_sort	Lloyd A C Chapman
title	Optimising cell aggregate expansion in a perfused hollow fibre bioreactor via mathematical modelling.
title_short	Optimising cell aggregate expansion in a perfused hollow fibre bioreactor via mathematical modelling.
title_full	Optimising cell aggregate expansion in a perfused hollow fibre bioreactor via mathematical modelling.
title_fullStr	Optimising cell aggregate expansion in a perfused hollow fibre bioreactor via mathematical modelling.
title_full_unstemmed	Optimising cell aggregate expansion in a perfused hollow fibre bioreactor via mathematical modelling.
title_sort	optimising cell aggregate expansion in a perfused hollow fibre bioreactor via mathematical modelling.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/e8d7f704587d42848b1366cb2516c3ab
work_keys_str_mv	AT lloydacchapman optimisingcellaggregateexpansioninaperfusedhollowfibrebioreactorviamathematicalmodelling AT rebeccajshipley optimisingcellaggregateexpansioninaperfusedhollowfibrebioreactorviamathematicalmodelling AT jonathanpwhiteley optimisingcellaggregateexpansioninaperfusedhollowfibrebioreactorviamathematicalmodelling AT mariannejellis optimisingcellaggregateexpansioninaperfusedhollowfibrebioreactorviamathematicalmodelling AT helenmbyrne optimisingcellaggregateexpansioninaperfusedhollowfibrebioreactorviamathematicalmodelling AT sarahlwaters optimisingcellaggregateexpansioninaperfusedhollowfibrebioreactorviamathematicalmodelling
_version_	1718414253692551168

Optimising cell aggregate expansion in a perfused hollow fibre bioreactor via mathematical modelling.

Ejemplares similares