Characterization of stratum corneum molecular dynamics by natural-abundance ¹³C solid-state NMR.

Despite the enormous potential for pharmaceutical applications, there is still a lack of understanding of the molecular details that can contribute to increased permeability of the stratum corneum (SC). To investigate the influence of hydration and heating on the SC, we record the natural-abundance...

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Autores principales:	Sebastian Björklund, Agnieszka Nowacka, Joke A Bouwstra, Emma Sparr, Daniel Topgaard
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e8d93c72757f4c31bf0a415596136e8a
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spelling	oai:doaj.org-article:e8d93c72757f4c31bf0a415596136e8a2021-11-18T07:48:23ZCharacterization of stratum corneum molecular dynamics by natural-abundance ¹³C solid-state NMR.1932-620310.1371/journal.pone.0061889https://doaj.org/article/e8d93c72757f4c31bf0a415596136e8a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23626744/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Despite the enormous potential for pharmaceutical applications, there is still a lack of understanding of the molecular details that can contribute to increased permeability of the stratum corneum (SC). To investigate the influence of hydration and heating on the SC, we record the natural-abundance (13)C signal of SC using polarization transfer solid-state NMR methods. Resonance lines from all major SC components are assigned. Comparison of the signal intensities obtained with the INEPT and CP pulse sequences gives information on the molecular dynamics of SC components. The majority of the lipids are rigid at 32°C, and those lipids co-exist with a small pool of mobile lipids. The ratio between mobile and rigid lipids increases with hydration. An abrupt change of keratin filament dynamics occurs at RH = 80-85%, from completely rigid to a structure with rigid backbone and mobile protruding terminals. Heating has a strong effect on the lipid mobility, but only a weak influence on the keratin filaments. The results provide novel molecular insight into how the SC constituents are affected by hydration and heating, and improve the understanding of enhanced SC permeability, which is associated with elevated temperatures and SC hydration.Sebastian BjörklundAgnieszka NowackaJoke A BouwstraEmma SparrDaniel TopgaardPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e61889 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sebastian Björklund Agnieszka Nowacka Joke A Bouwstra Emma Sparr Daniel Topgaard Characterization of stratum corneum molecular dynamics by natural-abundance ¹³C solid-state NMR.
description	Despite the enormous potential for pharmaceutical applications, there is still a lack of understanding of the molecular details that can contribute to increased permeability of the stratum corneum (SC). To investigate the influence of hydration and heating on the SC, we record the natural-abundance (13)C signal of SC using polarization transfer solid-state NMR methods. Resonance lines from all major SC components are assigned. Comparison of the signal intensities obtained with the INEPT and CP pulse sequences gives information on the molecular dynamics of SC components. The majority of the lipids are rigid at 32°C, and those lipids co-exist with a small pool of mobile lipids. The ratio between mobile and rigid lipids increases with hydration. An abrupt change of keratin filament dynamics occurs at RH = 80-85%, from completely rigid to a structure with rigid backbone and mobile protruding terminals. Heating has a strong effect on the lipid mobility, but only a weak influence on the keratin filaments. The results provide novel molecular insight into how the SC constituents are affected by hydration and heating, and improve the understanding of enhanced SC permeability, which is associated with elevated temperatures and SC hydration.
format	article
author	Sebastian Björklund Agnieszka Nowacka Joke A Bouwstra Emma Sparr Daniel Topgaard
author_facet	Sebastian Björklund Agnieszka Nowacka Joke A Bouwstra Emma Sparr Daniel Topgaard
author_sort	Sebastian Björklund
title	Characterization of stratum corneum molecular dynamics by natural-abundance ¹³C solid-state NMR.
title_short	Characterization of stratum corneum molecular dynamics by natural-abundance ¹³C solid-state NMR.
title_full	Characterization of stratum corneum molecular dynamics by natural-abundance ¹³C solid-state NMR.
title_fullStr	Characterization of stratum corneum molecular dynamics by natural-abundance ¹³C solid-state NMR.
title_full_unstemmed	Characterization of stratum corneum molecular dynamics by natural-abundance ¹³C solid-state NMR.
title_sort	characterization of stratum corneum molecular dynamics by natural-abundance ¹³c solid-state nmr.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e8d93c72757f4c31bf0a415596136e8a
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Characterization of stratum corneum molecular dynamics by natural-abundance ¹³C solid-state NMR.

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