Comparing the results of manual and automated quantitative corneal neuroanalysing modules for beginners

Abstract This study aimed to evaluate the reliability of in vivo confocal microscopic neuroanalysis by beginners using manual and automated modules. Images of sub-basal corneal nerve plexus (SCNP) from 108 images of 18 healthy participants were analyzed by 7 beginner observers using manual (CCMetric...

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Autores principales:	Po-Ying Wu, Jo-Hsuan Wu, Yi-Ting Hsieh, Lin Chih-Chieh Chen, Ting Cheng, Po-Yi Wu, Bing-Jun Hsieh, Wei-Lun Huang, Sheng-Lung Huang, Wei-Li Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e8eae6e546ff4e17adabfa007472d81b
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spelling	oai:doaj.org-article:e8eae6e546ff4e17adabfa007472d81b2021-12-02T15:31:36ZComparing the results of manual and automated quantitative corneal neuroanalysing modules for beginners10.1038/s41598-021-97567-y2045-2322https://doaj.org/article/e8eae6e546ff4e17adabfa007472d81b2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97567-yhttps://doaj.org/toc/2045-2322Abstract This study aimed to evaluate the reliability of in vivo confocal microscopic neuroanalysis by beginners using manual and automated modules. Images of sub-basal corneal nerve plexus (SCNP) from 108 images of 18 healthy participants were analyzed by 7 beginner observers using manual (CCMetrics, [CCM]) and automated (ACCMetrics, [ACCM]) module. SCNP parameters analyzed included corneal nerve fiber density (NFD), corneal nerve branch density (NBD), corneal nerve fiber length (NFL), and tortuosity coefficient (TC). The intra-observer repeatability, inter-observer reliability, inter-module agreement, and left–right eye symmetry level of SCNP parameters were examined. All observers showed good intra-observer repeatability using CCM (intraclass correlation coefficient [ICC] > 0.60 for all), except when measuring TC. Two observers demonstrated especially excellent repeatability in analyzing NFD, NBD, and NFL using manual mode, indicating the quality of interpretation may still be observer-dependent. Among all SCNP parameters, NFL had the best inter-observer reliability (Spearman’s rank-sum correlation coefficient [SpCC] and ICC > 0.85 for the 3 original observers) and left–right symmetry level (SpCC and ICC > 0.60). In the additional analysis of inter-observer reliability using results by all 7 observers, only NFL showed good inter-observer reliability (ICC = 0.79). Compared with CCM measurements, values of ACCM measurements were significantly lower, implying a poor inter-module agreement. Our result suggested that performance of quantitative corneal neuroanalysis by beginners maybe acceptable, with NFL being the most reliable parameter, and automated method cannot fully replace manual work.Po-Ying WuJo-Hsuan WuYi-Ting HsiehLin Chih-Chieh ChenTing ChengPo-Yi WuBing-Jun HsiehWei-Lun HuangSheng-Lung HuangWei-Li ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Po-Ying Wu Jo-Hsuan Wu Yi-Ting Hsieh Lin Chih-Chieh Chen Ting Cheng Po-Yi Wu Bing-Jun Hsieh Wei-Lun Huang Sheng-Lung Huang Wei-Li Chen Comparing the results of manual and automated quantitative corneal neuroanalysing modules for beginners
description	Abstract This study aimed to evaluate the reliability of in vivo confocal microscopic neuroanalysis by beginners using manual and automated modules. Images of sub-basal corneal nerve plexus (SCNP) from 108 images of 18 healthy participants were analyzed by 7 beginner observers using manual (CCMetrics, [CCM]) and automated (ACCMetrics, [ACCM]) module. SCNP parameters analyzed included corneal nerve fiber density (NFD), corneal nerve branch density (NBD), corneal nerve fiber length (NFL), and tortuosity coefficient (TC). The intra-observer repeatability, inter-observer reliability, inter-module agreement, and left–right eye symmetry level of SCNP parameters were examined. All observers showed good intra-observer repeatability using CCM (intraclass correlation coefficient [ICC] > 0.60 for all), except when measuring TC. Two observers demonstrated especially excellent repeatability in analyzing NFD, NBD, and NFL using manual mode, indicating the quality of interpretation may still be observer-dependent. Among all SCNP parameters, NFL had the best inter-observer reliability (Spearman’s rank-sum correlation coefficient [SpCC] and ICC > 0.85 for the 3 original observers) and left–right symmetry level (SpCC and ICC > 0.60). In the additional analysis of inter-observer reliability using results by all 7 observers, only NFL showed good inter-observer reliability (ICC = 0.79). Compared with CCM measurements, values of ACCM measurements were significantly lower, implying a poor inter-module agreement. Our result suggested that performance of quantitative corneal neuroanalysis by beginners maybe acceptable, with NFL being the most reliable parameter, and automated method cannot fully replace manual work.
format	article
author	Po-Ying Wu Jo-Hsuan Wu Yi-Ting Hsieh Lin Chih-Chieh Chen Ting Cheng Po-Yi Wu Bing-Jun Hsieh Wei-Lun Huang Sheng-Lung Huang Wei-Li Chen
author_facet	Po-Ying Wu Jo-Hsuan Wu Yi-Ting Hsieh Lin Chih-Chieh Chen Ting Cheng Po-Yi Wu Bing-Jun Hsieh Wei-Lun Huang Sheng-Lung Huang Wei-Li Chen
author_sort	Po-Ying Wu
title	Comparing the results of manual and automated quantitative corneal neuroanalysing modules for beginners
title_short	Comparing the results of manual and automated quantitative corneal neuroanalysing modules for beginners
title_full	Comparing the results of manual and automated quantitative corneal neuroanalysing modules for beginners
title_fullStr	Comparing the results of manual and automated quantitative corneal neuroanalysing modules for beginners
title_full_unstemmed	Comparing the results of manual and automated quantitative corneal neuroanalysing modules for beginners
title_sort	comparing the results of manual and automated quantitative corneal neuroanalysing modules for beginners
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e8eae6e546ff4e17adabfa007472d81b
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Comparing the results of manual and automated quantitative corneal neuroanalysing modules for beginners

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