Detection of CWD prions in naturally infected white-tailed deer fetuses and gestational tissues by PMCA

Abstract Chronic wasting disease (CWD) is a prevalent prion disease affecting cervids. CWD is thought to be transmitted through direct animal contact or by indirect exposure to contaminated environmental fomites. Other mechanisms of propagation such as vertical and maternal transmissions have also b...

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Autores principales:	Francisca Bravo-Risi, Paulina Soto, Thomas Eckland, Robert Dittmar, Santiago Ramírez, Celso S. G. Catumbela, Claudio Soto, Mitch Lockwood, Tracy Nichols, Rodrigo Morales
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e8f1a4655457452ab55c320ef40db9c4
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spelling	oai:doaj.org-article:e8f1a4655457452ab55c320ef40db9c42021-12-02T17:24:11ZDetection of CWD prions in naturally infected white-tailed deer fetuses and gestational tissues by PMCA10.1038/s41598-021-97737-y2045-2322https://doaj.org/article/e8f1a4655457452ab55c320ef40db9c42021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97737-yhttps://doaj.org/toc/2045-2322Abstract Chronic wasting disease (CWD) is a prevalent prion disease affecting cervids. CWD is thought to be transmitted through direct animal contact or by indirect exposure to contaminated environmental fomites. Other mechanisms of propagation such as vertical and maternal transmissions have also been suggested using naturally and experimentally infected animals. Here, we describe the detection of CWD prions in naturally-infected, farmed white-tailed deer (WTD) fetal tissues using the Protein Misfolding Cyclic Amplification (PMCA) technique. Prion seeding activity was identified in a variety of gestational and fetal tissues. Future studies should demonstrate if prions present in fetuses are at sufficient quantities to cause CWD after birth. This data confirms previous findings in other animal species and furthers vertical transmission as a relevant mechanism of CWD dissemination.Francisca Bravo-RisiPaulina SotoThomas EcklandRobert DittmarSantiago RamírezCelso S. G. CatumbelaClaudio SotoMitch LockwoodTracy NicholsRodrigo MoralesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Francisca Bravo-Risi Paulina Soto Thomas Eckland Robert Dittmar Santiago Ramírez Celso S. G. Catumbela Claudio Soto Mitch Lockwood Tracy Nichols Rodrigo Morales Detection of CWD prions in naturally infected white-tailed deer fetuses and gestational tissues by PMCA
description	Abstract Chronic wasting disease (CWD) is a prevalent prion disease affecting cervids. CWD is thought to be transmitted through direct animal contact or by indirect exposure to contaminated environmental fomites. Other mechanisms of propagation such as vertical and maternal transmissions have also been suggested using naturally and experimentally infected animals. Here, we describe the detection of CWD prions in naturally-infected, farmed white-tailed deer (WTD) fetal tissues using the Protein Misfolding Cyclic Amplification (PMCA) technique. Prion seeding activity was identified in a variety of gestational and fetal tissues. Future studies should demonstrate if prions present in fetuses are at sufficient quantities to cause CWD after birth. This data confirms previous findings in other animal species and furthers vertical transmission as a relevant mechanism of CWD dissemination.
format	article
author	Francisca Bravo-Risi Paulina Soto Thomas Eckland Robert Dittmar Santiago Ramírez Celso S. G. Catumbela Claudio Soto Mitch Lockwood Tracy Nichols Rodrigo Morales
author_facet	Francisca Bravo-Risi Paulina Soto Thomas Eckland Robert Dittmar Santiago Ramírez Celso S. G. Catumbela Claudio Soto Mitch Lockwood Tracy Nichols Rodrigo Morales
author_sort	Francisca Bravo-Risi
title	Detection of CWD prions in naturally infected white-tailed deer fetuses and gestational tissues by PMCA
title_short	Detection of CWD prions in naturally infected white-tailed deer fetuses and gestational tissues by PMCA
title_full	Detection of CWD prions in naturally infected white-tailed deer fetuses and gestational tissues by PMCA
title_fullStr	Detection of CWD prions in naturally infected white-tailed deer fetuses and gestational tissues by PMCA
title_full_unstemmed	Detection of CWD prions in naturally infected white-tailed deer fetuses and gestational tissues by PMCA
title_sort	detection of cwd prions in naturally infected white-tailed deer fetuses and gestational tissues by pmca
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e8f1a4655457452ab55c320ef40db9c4
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Detection of CWD prions in naturally infected white-tailed deer fetuses and gestational tissues by PMCA

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