Heat-Killed Lactobacilli Preparations Promote Healing in the Experimental Cutaneous Wounds

Probiotics are defined as microorganisms with beneficial health effects when consumed by humans, being applied mainly to improve allergic or intestinal diseases. Due to the increasing resistance of pathogens to antibiotics, the abuse of antibiotics becomes inefficient in the skin and in systemic inf...

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Autores principales:	Wan-Hua Tsai, Chia-Hsuan Chou, Tsuei-Yin Huang, Hui-Ling Wang, Peng-Ju Chien, Wen-Wei Chang, Hsueh-Te Lee
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	heat-killed probiotics Lactobacillus <i>L. plantarum</i> <i>L. paracasei</i> skin wound healing lipoteichoic acid Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e8fd8080ecb1472e995a6b75ca5f0e71
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spelling	oai:doaj.org-article:e8fd8080ecb1472e995a6b75ca5f0e712021-11-25T17:13:26ZHeat-Killed Lactobacilli Preparations Promote Healing in the Experimental Cutaneous Wounds10.3390/cells101132642073-4409https://doaj.org/article/e8fd8080ecb1472e995a6b75ca5f0e712021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3264https://doaj.org/toc/2073-4409Probiotics are defined as microorganisms with beneficial health effects when consumed by humans, being applied mainly to improve allergic or intestinal diseases. Due to the increasing resistance of pathogens to antibiotics, the abuse of antibiotics becomes inefficient in the skin and in systemic infections, and probiotics may also provide the protective effect for repairing the healing of infected cutaneous wounds. Here we selected two Lactobacillus strains, <i>L. plantarum</i> GMNL-6 and <i>L. paracasei</i> GMNL-653, in heat-killed format to examine the beneficial effect in skin wound repair through the selection by promoting collagen synthesis in Hs68 fibroblast cells. The coverage of gels containing heat-killed GMNL-6 or GMNL-653 on the mouse tail with experimental wounds displayed healing promoting effects with promoting of metalloproteinase-1 expression at the early phase and reduced excessive fibrosis accumulation and deposition in the later tail-skin recovery stage. More importantly, lipoteichoic acid, the major component of Lactobacillus cell wall, from GMNL-6/GMNL-653 could achieve the anti-fibrogenic benefit similar to the heat-killed bacteria cells in the TGF-β stimulated Hs68 fibroblast cell model. Our study offers a new therapeutic potential of the heat-killed format of Lactobacillus as an alternative approach to treating skin healing disorders.Wan-Hua TsaiChia-Hsuan ChouTsuei-Yin HuangHui-Ling WangPeng-Ju ChienWen-Wei ChangHsueh-Te LeeMDPI AGarticleheat-killed probioticsLactobacillus<i>L. plantarum</i><i>L. paracasei</i>skin wound healinglipoteichoic acidBiology (General)QH301-705.5ENCells, Vol 10, Iss 3264, p 3264 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	heat-killed probiotics Lactobacillus <i>L. plantarum</i> <i>L. paracasei</i> skin wound healing lipoteichoic acid Biology (General) QH301-705.5
spellingShingle	heat-killed probiotics Lactobacillus <i>L. plantarum</i> <i>L. paracasei</i> skin wound healing lipoteichoic acid Biology (General) QH301-705.5 Wan-Hua Tsai Chia-Hsuan Chou Tsuei-Yin Huang Hui-Ling Wang Peng-Ju Chien Wen-Wei Chang Hsueh-Te Lee Heat-Killed Lactobacilli Preparations Promote Healing in the Experimental Cutaneous Wounds
description	Probiotics are defined as microorganisms with beneficial health effects when consumed by humans, being applied mainly to improve allergic or intestinal diseases. Due to the increasing resistance of pathogens to antibiotics, the abuse of antibiotics becomes inefficient in the skin and in systemic infections, and probiotics may also provide the protective effect for repairing the healing of infected cutaneous wounds. Here we selected two Lactobacillus strains, <i>L. plantarum</i> GMNL-6 and <i>L. paracasei</i> GMNL-653, in heat-killed format to examine the beneficial effect in skin wound repair through the selection by promoting collagen synthesis in Hs68 fibroblast cells. The coverage of gels containing heat-killed GMNL-6 or GMNL-653 on the mouse tail with experimental wounds displayed healing promoting effects with promoting of metalloproteinase-1 expression at the early phase and reduced excessive fibrosis accumulation and deposition in the later tail-skin recovery stage. More importantly, lipoteichoic acid, the major component of Lactobacillus cell wall, from GMNL-6/GMNL-653 could achieve the anti-fibrogenic benefit similar to the heat-killed bacteria cells in the TGF-β stimulated Hs68 fibroblast cell model. Our study offers a new therapeutic potential of the heat-killed format of Lactobacillus as an alternative approach to treating skin healing disorders.
format	article
author	Wan-Hua Tsai Chia-Hsuan Chou Tsuei-Yin Huang Hui-Ling Wang Peng-Ju Chien Wen-Wei Chang Hsueh-Te Lee
author_facet	Wan-Hua Tsai Chia-Hsuan Chou Tsuei-Yin Huang Hui-Ling Wang Peng-Ju Chien Wen-Wei Chang Hsueh-Te Lee
author_sort	Wan-Hua Tsai
title	Heat-Killed Lactobacilli Preparations Promote Healing in the Experimental Cutaneous Wounds
title_short	Heat-Killed Lactobacilli Preparations Promote Healing in the Experimental Cutaneous Wounds
title_full	Heat-Killed Lactobacilli Preparations Promote Healing in the Experimental Cutaneous Wounds
title_fullStr	Heat-Killed Lactobacilli Preparations Promote Healing in the Experimental Cutaneous Wounds
title_full_unstemmed	Heat-Killed Lactobacilli Preparations Promote Healing in the Experimental Cutaneous Wounds
title_sort	heat-killed lactobacilli preparations promote healing in the experimental cutaneous wounds
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e8fd8080ecb1472e995a6b75ca5f0e71
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Heat-Killed Lactobacilli Preparations Promote Healing in the Experimental Cutaneous Wounds

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