In depth characterisation of the biomolecular coronas of polymer coated inorganic nanoparticles with differential centrifugal sedimentation
Abstract Advances in nanofabrication methods have enabled the tailoring of new strategies towards the controlled production of nanoparticles with attractive applications in healthcare. In many cases, their characterisation remains a big challenge, particularly for small-sized functional nanoparticle...
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Nature Portfolio
2021
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oai:doaj.org-article:e9027f39b1224ff3aefbda2eb30b21162021-12-02T16:30:58ZIn depth characterisation of the biomolecular coronas of polymer coated inorganic nanoparticles with differential centrifugal sedimentation10.1038/s41598-021-84029-82045-2322https://doaj.org/article/e9027f39b1224ff3aefbda2eb30b21162021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84029-8https://doaj.org/toc/2045-2322Abstract Advances in nanofabrication methods have enabled the tailoring of new strategies towards the controlled production of nanoparticles with attractive applications in healthcare. In many cases, their characterisation remains a big challenge, particularly for small-sized functional nanoparticles of 5 nm diameter or smaller, where current particle sizing techniques struggle to provide the required sensitivity and accuracy. There is a clear need for the development of new reliable characterisation approaches for the physico-chemical characterisation of nanoparticles with significant accuracy, particularly for the analysis of the particles in the presence of complex biological fluids. Herein, we show that the Differential Centrifugal Sedimentation can be utilised as a high-precision tool for the reliable characterisation of functional nanoparticles of different materials. We report a method to correlate the sedimentation shift with the polymer and biomolecule adsorption on the nanoparticle surface, validating the developed core–shell model. We also highlight its limit when measuring nanoparticles of smaller size and the need to use several complementary methods when characterising nanoparticle corona complexes.André Perez-PottiHender LopezBeatriz PelazAbuelmagd AbdelmonemMahmoud G. SolimanIngmar SchoenPhilip M. KellyKenneth A. DawsonWolfgang J. ParakZeljka KrpeticMarco P. MonopoliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
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Medicine R Science Q |
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Medicine R Science Q André Perez-Potti Hender Lopez Beatriz Pelaz Abuelmagd Abdelmonem Mahmoud G. Soliman Ingmar Schoen Philip M. Kelly Kenneth A. Dawson Wolfgang J. Parak Zeljka Krpetic Marco P. Monopoli In depth characterisation of the biomolecular coronas of polymer coated inorganic nanoparticles with differential centrifugal sedimentation |
| description |
Abstract Advances in nanofabrication methods have enabled the tailoring of new strategies towards the controlled production of nanoparticles with attractive applications in healthcare. In many cases, their characterisation remains a big challenge, particularly for small-sized functional nanoparticles of 5 nm diameter or smaller, where current particle sizing techniques struggle to provide the required sensitivity and accuracy. There is a clear need for the development of new reliable characterisation approaches for the physico-chemical characterisation of nanoparticles with significant accuracy, particularly for the analysis of the particles in the presence of complex biological fluids. Herein, we show that the Differential Centrifugal Sedimentation can be utilised as a high-precision tool for the reliable characterisation of functional nanoparticles of different materials. We report a method to correlate the sedimentation shift with the polymer and biomolecule adsorption on the nanoparticle surface, validating the developed core–shell model. We also highlight its limit when measuring nanoparticles of smaller size and the need to use several complementary methods when characterising nanoparticle corona complexes. |
| format |
article |
| author |
André Perez-Potti Hender Lopez Beatriz Pelaz Abuelmagd Abdelmonem Mahmoud G. Soliman Ingmar Schoen Philip M. Kelly Kenneth A. Dawson Wolfgang J. Parak Zeljka Krpetic Marco P. Monopoli |
| author_facet |
André Perez-Potti Hender Lopez Beatriz Pelaz Abuelmagd Abdelmonem Mahmoud G. Soliman Ingmar Schoen Philip M. Kelly Kenneth A. Dawson Wolfgang J. Parak Zeljka Krpetic Marco P. Monopoli |
| author_sort |
André Perez-Potti |
| title |
In depth characterisation of the biomolecular coronas of polymer coated inorganic nanoparticles with differential centrifugal sedimentation |
| title_short |
In depth characterisation of the biomolecular coronas of polymer coated inorganic nanoparticles with differential centrifugal sedimentation |
| title_full |
In depth characterisation of the biomolecular coronas of polymer coated inorganic nanoparticles with differential centrifugal sedimentation |
| title_fullStr |
In depth characterisation of the biomolecular coronas of polymer coated inorganic nanoparticles with differential centrifugal sedimentation |
| title_full_unstemmed |
In depth characterisation of the biomolecular coronas of polymer coated inorganic nanoparticles with differential centrifugal sedimentation |
| title_sort |
in depth characterisation of the biomolecular coronas of polymer coated inorganic nanoparticles with differential centrifugal sedimentation |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/e9027f39b1224ff3aefbda2eb30b2116 |
| work_keys_str_mv |
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1718383905444200448 |