Nanocalcium-deficient hydroxyapatite–poly (ε-caprolactone)–polyethylene glycol–poly (ε-caprolactone) composite scaffolds
Zhiwei Wang,* Ming Li,* Baoqing Yu, Liehu Cao, Qingsong Yang, Jiacan SuDepartment of Orthopedics, Shanghai Hospital, Second Military Medical University, Shanghai, People's Republic of China *These authors contributed equally to this workAbstract: A bioactive composite of nano calcium-def...
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Dove Medical Press
2012
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oai:doaj.org-article:e90a839485104a8e92a076fd16fbf0292021-12-02T06:31:45ZNanocalcium-deficient hydroxyapatite–poly (ε-caprolactone)–polyethylene glycol–poly (ε-caprolactone) composite scaffolds1176-91141178-2013https://doaj.org/article/e90a839485104a8e92a076fd16fbf0292012-07-01T00:00:00Zhttp://www.dovepress.com/nanocalcium-deficient-hydroxyapatitendashpoly-epsilon-caprolactonendas-a10350https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Zhiwei Wang,* Ming Li,* Baoqing Yu, Liehu Cao, Qingsong Yang, Jiacan SuDepartment of Orthopedics, Shanghai Hospital, Second Military Medical University, Shanghai, People's Republic of China *These authors contributed equally to this workAbstract: A bioactive composite of nano calcium-deficient apatite (n-CDAP) with an atom molar ratio of calcium to phosphate (Ca/P) of 1.50 and poly(ε-caprolactone)–poly(ethylene glycol)–poly(ε-caprolactone) (PCL–PEG–PCL) was synthesized, and a composite scaffold was fabricated. The composite scaffolds with 40 wt% n-CDAP contained well interconnected macropores around 400 µm, and exhibited a porosity of 75%. The weight-loss ratio of the n-CDAP/PCL–PEG–PCL was significantly greater than nano hydroxyapatite (n-HA, Ca/P = 1.67)/PCL–PEG–PCL composite scaffolds during soaking into phosphate-buffered saline (pH 7.4) for 70 days, indicating that n-CDAP-based composite had good degradability compared with n-HA. The viability ratio of MG-63 cells was significantly higher on n-CDAP than n-HA-based composite scaffolds at 3 and 5 days. In addition, the alkaline phosphatase activity of the MG-63 cells cultured on n-CDAP was higher than n-HA-based composite scaffolds at 7 days. Histological evaluation showed that the introduction of n-CDAP into PCL–PEG–PCL enhanced the efficiency of new bone formation when the composite scaffolds were implanted into rabbit bone defects. The results suggested that the n-CDAP-based composite exhibits good biocompatibility, biodegradation, and osteogenesis in vivo.Keywords: nano calcium-deficient apatite, composite scaffold, degradability, cell responses, osteogenesisWang ZLi MYu BCao LYang QSu JDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3123-3131 (2012) |
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Medicine (General) R5-920 |
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Medicine (General) R5-920 Wang Z Li M Yu B Cao L Yang Q Su J Nanocalcium-deficient hydroxyapatite–poly (ε-caprolactone)–polyethylene glycol–poly (ε-caprolactone) composite scaffolds |
| description |
Zhiwei Wang,* Ming Li,* Baoqing Yu, Liehu Cao, Qingsong Yang, Jiacan SuDepartment of Orthopedics, Shanghai Hospital, Second Military Medical University, Shanghai, People's Republic of China *These authors contributed equally to this workAbstract: A bioactive composite of nano calcium-deficient apatite (n-CDAP) with an atom molar ratio of calcium to phosphate (Ca/P) of 1.50 and poly(ε-caprolactone)–poly(ethylene glycol)–poly(ε-caprolactone) (PCL–PEG–PCL) was synthesized, and a composite scaffold was fabricated. The composite scaffolds with 40 wt% n-CDAP contained well interconnected macropores around 400 µm, and exhibited a porosity of 75%. The weight-loss ratio of the n-CDAP/PCL–PEG–PCL was significantly greater than nano hydroxyapatite (n-HA, Ca/P = 1.67)/PCL–PEG–PCL composite scaffolds during soaking into phosphate-buffered saline (pH 7.4) for 70 days, indicating that n-CDAP-based composite had good degradability compared with n-HA. The viability ratio of MG-63 cells was significantly higher on n-CDAP than n-HA-based composite scaffolds at 3 and 5 days. In addition, the alkaline phosphatase activity of the MG-63 cells cultured on n-CDAP was higher than n-HA-based composite scaffolds at 7 days. Histological evaluation showed that the introduction of n-CDAP into PCL–PEG–PCL enhanced the efficiency of new bone formation when the composite scaffolds were implanted into rabbit bone defects. The results suggested that the n-CDAP-based composite exhibits good biocompatibility, biodegradation, and osteogenesis in vivo.Keywords: nano calcium-deficient apatite, composite scaffold, degradability, cell responses, osteogenesis |
| format |
article |
| author |
Wang Z Li M Yu B Cao L Yang Q Su J |
| author_facet |
Wang Z Li M Yu B Cao L Yang Q Su J |
| author_sort |
Wang Z |
| title |
Nanocalcium-deficient hydroxyapatite–poly (ε-caprolactone)–polyethylene glycol–poly (ε-caprolactone) composite scaffolds |
| title_short |
Nanocalcium-deficient hydroxyapatite–poly (ε-caprolactone)–polyethylene glycol–poly (ε-caprolactone) composite scaffolds |
| title_full |
Nanocalcium-deficient hydroxyapatite–poly (ε-caprolactone)–polyethylene glycol–poly (ε-caprolactone) composite scaffolds |
| title_fullStr |
Nanocalcium-deficient hydroxyapatite–poly (ε-caprolactone)–polyethylene glycol–poly (ε-caprolactone) composite scaffolds |
| title_full_unstemmed |
Nanocalcium-deficient hydroxyapatite–poly (ε-caprolactone)–polyethylene glycol–poly (ε-caprolactone) composite scaffolds |
| title_sort |
nanocalcium-deficient hydroxyapatite–poly (ε-caprolactone)–polyethylene glycol–poly (ε-caprolactone) composite scaffolds |
| publisher |
Dove Medical Press |
| publishDate |
2012 |
| url |
https://doaj.org/article/e90a839485104a8e92a076fd16fbf029 |
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