Extended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymph

Abstract Ascoviruses are large dsDNA viruses characterized by the extraordinary changes they induce in cellular pathogenesis and architecture whereby after nuclear lysis and extensive hypertrophy, each cell is cleaved into numerous vesicles for virion reproduction. However, the level of viral replic...

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Autores principales:	Heba A. H. Zaghloul, Robert H. Hice, Peter Arensburger, Dennis K. Bideshi, Brian A. Federici
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e90ac31c1d04490aab7621f34d92506f
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spelling	oai:doaj.org-article:e90ac31c1d04490aab7621f34d92506f2021-12-02T16:27:50ZExtended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymph10.1038/s41598-021-95553-y2045-2322https://doaj.org/article/e90ac31c1d04490aab7621f34d92506f2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95553-yhttps://doaj.org/toc/2045-2322Abstract Ascoviruses are large dsDNA viruses characterized by the extraordinary changes they induce in cellular pathogenesis and architecture whereby after nuclear lysis and extensive hypertrophy, each cell is cleaved into numerous vesicles for virion reproduction. However, the level of viral replication and transcription in vesicles compared to other host tissues remains uncertain. Therefore, we applied RNA-Sequencing to compare the temporal transcriptome of Spodoptera frugiperda ascovirus (SfAV) and Trichoplusia ni ascovirus (TnAV) at 7, 14, and 21 days post-infection (dpi). We found most transcription occurred in viral vesicles, not in initial tissues infected, a remarkably novel reproduction mechanism compared to all other viruses and most other intracellular pathogens. Specifically, the highest level of viral gene expression occurred in hemolymph, for TnAV at 7 dpi, and SfAV at 14 dpi. Moreover, we found that host immune genes were partially down-regulated in hemolymph, where most viral replication occurred in highly dense accumulations of vesicles.Heba A. H. ZaghloulRobert H. HicePeter ArensburgerDennis K. BideshiBrian A. FedericiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Heba A. H. Zaghloul Robert H. Hice Peter Arensburger Dennis K. Bideshi Brian A. Federici Extended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymph
description	Abstract Ascoviruses are large dsDNA viruses characterized by the extraordinary changes they induce in cellular pathogenesis and architecture whereby after nuclear lysis and extensive hypertrophy, each cell is cleaved into numerous vesicles for virion reproduction. However, the level of viral replication and transcription in vesicles compared to other host tissues remains uncertain. Therefore, we applied RNA-Sequencing to compare the temporal transcriptome of Spodoptera frugiperda ascovirus (SfAV) and Trichoplusia ni ascovirus (TnAV) at 7, 14, and 21 days post-infection (dpi). We found most transcription occurred in viral vesicles, not in initial tissues infected, a remarkably novel reproduction mechanism compared to all other viruses and most other intracellular pathogens. Specifically, the highest level of viral gene expression occurred in hemolymph, for TnAV at 7 dpi, and SfAV at 14 dpi. Moreover, we found that host immune genes were partially down-regulated in hemolymph, where most viral replication occurred in highly dense accumulations of vesicles.
format	article
author	Heba A. H. Zaghloul Robert H. Hice Peter Arensburger Dennis K. Bideshi Brian A. Federici
author_facet	Heba A. H. Zaghloul Robert H. Hice Peter Arensburger Dennis K. Bideshi Brian A. Federici
author_sort	Heba A. H. Zaghloul
title	Extended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymph
title_short	Extended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymph
title_full	Extended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymph
title_fullStr	Extended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymph
title_full_unstemmed	Extended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymph
title_sort	extended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymph
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e90ac31c1d04490aab7621f34d92506f
work_keys_str_mv	AT hebaahzaghloul extendedinvivotranscriptomesoftwoascoviruseswithdifferenttissuetropismsrevealalternativemechanismsforenhancingvirusreproductioninhemolymph AT roberthhice extendedinvivotranscriptomesoftwoascoviruseswithdifferenttissuetropismsrevealalternativemechanismsforenhancingvirusreproductioninhemolymph AT peterarensburger extendedinvivotranscriptomesoftwoascoviruseswithdifferenttissuetropismsrevealalternativemechanismsforenhancingvirusreproductioninhemolymph AT denniskbideshi extendedinvivotranscriptomesoftwoascoviruseswithdifferenttissuetropismsrevealalternativemechanismsforenhancingvirusreproductioninhemolymph AT brianafederici extendedinvivotranscriptomesoftwoascoviruseswithdifferenttissuetropismsrevealalternativemechanismsforenhancingvirusreproductioninhemolymph
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Extended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymph

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