Cerebrospinal fluid (CSF) augments metabolism and virulence expression factors in Acinetobacter baumannii
Abstract In a recent report by the Centers for Disease Control and Prevention (CDC), multidrug resistant (MDR) Acinetobacter baumannii is a pathogen described as an “urgent threat.” Infection with this bacterium manifests as different diseases such as community and nosocomial pneumonia, bloodstream...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/e90f21769c5941c48c34928a84a7b5de |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:e90f21769c5941c48c34928a84a7b5de |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:e90f21769c5941c48c34928a84a7b5de2021-12-02T13:30:11ZCerebrospinal fluid (CSF) augments metabolism and virulence expression factors in Acinetobacter baumannii10.1038/s41598-021-81714-62045-2322https://doaj.org/article/e90f21769c5941c48c34928a84a7b5de2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81714-6https://doaj.org/toc/2045-2322Abstract In a recent report by the Centers for Disease Control and Prevention (CDC), multidrug resistant (MDR) Acinetobacter baumannii is a pathogen described as an “urgent threat.” Infection with this bacterium manifests as different diseases such as community and nosocomial pneumonia, bloodstream infections, endocarditis, infections of the urinary tract, wound infections, burn infections, skin and soft tissue infections, and meningitis. In particular, nosocomial meningitis, an unwelcome complication of neurosurgery caused by extensively-drug resistant (XDR) A. baumannii, is extremely challenging to manage. Therefore, understanding how A. baumannii adapts to different host environments, such as cerebrospinal fluid (CSF) that may trigger changes in expression of virulence factors that are associated with the successful establishment and progress of this infection is necessary. The present in-vitro work describes, the genetic changes that occur during A. baumannii infiltration into CSF and displays A. baumannii’s expansive versatility to persist in a nutrient limited environment while enhancing several virulence factors to survive and persist. While a hypervirulent A. baumannii strain did not show changes in its transcriptome when incubated in the presence of CSF, a low-virulence isolate showed significant differences in gene expression and phenotypic traits. Exposure to 4% CSF caused increased expression of virulence factors such as fimbriae, pilins, and iron chelators, and other virulence determinants that was confirmed in various model systems. Furthermore, although CSF's presence did not enhance bacterial growth, an increase of expression of genes encoding transcription, translation, and the ATP synthesis machinery was observed. This work also explores A. baumannii’s response to an essential component, human serum albumin (HSA), within CSF to trigger the differential expression of genes associated with its pathoadaptibility in this environment.Jasmine MartinezChelsea Razo-GutierrezCasin LeRobert CourvilleCamila PimentelChristine LiuSammie E. FungMarisel R. TuttobeneKimberly PhanAlejandro J. VilaParvin ShahrestaniVeronica JimenezMarcelo E. TolmaskyScott A. BeckaKrisztina M. Papp-WallaceRobert A. BonomoAlfonso Soler-BistueRodrigo SieiraMaria Soledad RamirezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Jasmine Martinez Chelsea Razo-Gutierrez Casin Le Robert Courville Camila Pimentel Christine Liu Sammie E. Fung Marisel R. Tuttobene Kimberly Phan Alejandro J. Vila Parvin Shahrestani Veronica Jimenez Marcelo E. Tolmasky Scott A. Becka Krisztina M. Papp-Wallace Robert A. Bonomo Alfonso Soler-Bistue Rodrigo Sieira Maria Soledad Ramirez Cerebrospinal fluid (CSF) augments metabolism and virulence expression factors in Acinetobacter baumannii |
| description |
Abstract In a recent report by the Centers for Disease Control and Prevention (CDC), multidrug resistant (MDR) Acinetobacter baumannii is a pathogen described as an “urgent threat.” Infection with this bacterium manifests as different diseases such as community and nosocomial pneumonia, bloodstream infections, endocarditis, infections of the urinary tract, wound infections, burn infections, skin and soft tissue infections, and meningitis. In particular, nosocomial meningitis, an unwelcome complication of neurosurgery caused by extensively-drug resistant (XDR) A. baumannii, is extremely challenging to manage. Therefore, understanding how A. baumannii adapts to different host environments, such as cerebrospinal fluid (CSF) that may trigger changes in expression of virulence factors that are associated with the successful establishment and progress of this infection is necessary. The present in-vitro work describes, the genetic changes that occur during A. baumannii infiltration into CSF and displays A. baumannii’s expansive versatility to persist in a nutrient limited environment while enhancing several virulence factors to survive and persist. While a hypervirulent A. baumannii strain did not show changes in its transcriptome when incubated in the presence of CSF, a low-virulence isolate showed significant differences in gene expression and phenotypic traits. Exposure to 4% CSF caused increased expression of virulence factors such as fimbriae, pilins, and iron chelators, and other virulence determinants that was confirmed in various model systems. Furthermore, although CSF's presence did not enhance bacterial growth, an increase of expression of genes encoding transcription, translation, and the ATP synthesis machinery was observed. This work also explores A. baumannii’s response to an essential component, human serum albumin (HSA), within CSF to trigger the differential expression of genes associated with its pathoadaptibility in this environment. |
| format |
article |
| author |
Jasmine Martinez Chelsea Razo-Gutierrez Casin Le Robert Courville Camila Pimentel Christine Liu Sammie E. Fung Marisel R. Tuttobene Kimberly Phan Alejandro J. Vila Parvin Shahrestani Veronica Jimenez Marcelo E. Tolmasky Scott A. Becka Krisztina M. Papp-Wallace Robert A. Bonomo Alfonso Soler-Bistue Rodrigo Sieira Maria Soledad Ramirez |
| author_facet |
Jasmine Martinez Chelsea Razo-Gutierrez Casin Le Robert Courville Camila Pimentel Christine Liu Sammie E. Fung Marisel R. Tuttobene Kimberly Phan Alejandro J. Vila Parvin Shahrestani Veronica Jimenez Marcelo E. Tolmasky Scott A. Becka Krisztina M. Papp-Wallace Robert A. Bonomo Alfonso Soler-Bistue Rodrigo Sieira Maria Soledad Ramirez |
| author_sort |
Jasmine Martinez |
| title |
Cerebrospinal fluid (CSF) augments metabolism and virulence expression factors in Acinetobacter baumannii |
| title_short |
Cerebrospinal fluid (CSF) augments metabolism and virulence expression factors in Acinetobacter baumannii |
| title_full |
Cerebrospinal fluid (CSF) augments metabolism and virulence expression factors in Acinetobacter baumannii |
| title_fullStr |
Cerebrospinal fluid (CSF) augments metabolism and virulence expression factors in Acinetobacter baumannii |
| title_full_unstemmed |
Cerebrospinal fluid (CSF) augments metabolism and virulence expression factors in Acinetobacter baumannii |
| title_sort |
cerebrospinal fluid (csf) augments metabolism and virulence expression factors in acinetobacter baumannii |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/e90f21769c5941c48c34928a84a7b5de |
| work_keys_str_mv |
AT jasminemartinez cerebrospinalfluidcsfaugmentsmetabolismandvirulenceexpressionfactorsinacinetobacterbaumannii AT chelsearazogutierrez cerebrospinalfluidcsfaugmentsmetabolismandvirulenceexpressionfactorsinacinetobacterbaumannii AT casinle cerebrospinalfluidcsfaugmentsmetabolismandvirulenceexpressionfactorsinacinetobacterbaumannii AT robertcourville cerebrospinalfluidcsfaugmentsmetabolismandvirulenceexpressionfactorsinacinetobacterbaumannii AT camilapimentel cerebrospinalfluidcsfaugmentsmetabolismandvirulenceexpressionfactorsinacinetobacterbaumannii AT christineliu cerebrospinalfluidcsfaugmentsmetabolismandvirulenceexpressionfactorsinacinetobacterbaumannii AT sammieefung cerebrospinalfluidcsfaugmentsmetabolismandvirulenceexpressionfactorsinacinetobacterbaumannii AT mariselrtuttobene cerebrospinalfluidcsfaugmentsmetabolismandvirulenceexpressionfactorsinacinetobacterbaumannii AT kimberlyphan cerebrospinalfluidcsfaugmentsmetabolismandvirulenceexpressionfactorsinacinetobacterbaumannii AT alejandrojvila cerebrospinalfluidcsfaugmentsmetabolismandvirulenceexpressionfactorsinacinetobacterbaumannii AT parvinshahrestani cerebrospinalfluidcsfaugmentsmetabolismandvirulenceexpressionfactorsinacinetobacterbaumannii AT veronicajimenez cerebrospinalfluidcsfaugmentsmetabolismandvirulenceexpressionfactorsinacinetobacterbaumannii AT marceloetolmasky cerebrospinalfluidcsfaugmentsmetabolismandvirulenceexpressionfactorsinacinetobacterbaumannii AT scottabecka cerebrospinalfluidcsfaugmentsmetabolismandvirulenceexpressionfactorsinacinetobacterbaumannii AT krisztinampappwallace cerebrospinalfluidcsfaugmentsmetabolismandvirulenceexpressionfactorsinacinetobacterbaumannii AT robertabonomo cerebrospinalfluidcsfaugmentsmetabolismandvirulenceexpressionfactorsinacinetobacterbaumannii AT alfonsosolerbistue cerebrospinalfluidcsfaugmentsmetabolismandvirulenceexpressionfactorsinacinetobacterbaumannii AT rodrigosieira cerebrospinalfluidcsfaugmentsmetabolismandvirulenceexpressionfactorsinacinetobacterbaumannii AT mariasoledadramirez cerebrospinalfluidcsfaugmentsmetabolismandvirulenceexpressionfactorsinacinetobacterbaumannii |
| _version_ |
1718392947850870784 |