Isospora suis in an epithelial cell culture system - an in vitro model for sexual development in coccidia.

Coccidian parasites are of major importance in animal production, public health and food safety. The most frequently used representative in basic research on this group is Toxoplasma gondii. Although this parasite is well investigated there is no adequate in vitro model for its sexual development av...

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Autores principales:	Hanna Lucia Worliczek, Bärbel Ruttkowski, Lukas Schwarz, Kirsti Witter, Waltraud Tschulenk, Anja Joachim
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e9130874406847148235826567fcda92
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spelling	oai:doaj.org-article:e9130874406847148235826567fcda922021-11-18T07:38:20ZIsospora suis in an epithelial cell culture system - an in vitro model for sexual development in coccidia.1932-620310.1371/journal.pone.0069797https://doaj.org/article/e9130874406847148235826567fcda922013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23861983/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Coccidian parasites are of major importance in animal production, public health and food safety. The most frequently used representative in basic research on this group is Toxoplasma gondii. Although this parasite is well investigated there is no adequate in vitro model for its sexual development available and knowledge on this important life cycle phase is therefore scarce. The use of Isosporasuis, a sister taxon to T. gondii and the causative agent of piglet coccidiosis, could provide a solution for this. In the present study an in vitro model for neonatal porcine coccidiosis in cells representative for the in vivo situation in the piglet gut was developed and evaluated. The parasite development was investigated by light and transmission electron microscopy and optimum culture conditions were evaluated. Intestinal porcine epithelial cells (IPEC-J2) adequately representing the natural host cells supported the development of all endogenous life cycle stages of I. suis, including gametocytes and oocysts. A concentration of 5% fetal calf serum in the culture medium led to highest gametocyte densities on day 12 post infection. Low infection doses (≤1 sporozoite for 100 host cells) were best for oocyst and gametocyte development. The presented system can also be used for immunostaining with established antibodies developed against T. gondii (in our case, anti-TgIMC3 antibodies directed against the inner membrane complex 3). The complete life cycle of I. suis in a cell line representing the natural host cell type and species provides a unique model among coccidian parasites and can be used to address a wide range of topics, especially with regard to the sexual development of coccidia.Hanna Lucia WorliczekBärbel RuttkowskiLukas SchwarzKirsti WitterWaltraud TschulenkAnja JoachimPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e69797 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hanna Lucia Worliczek Bärbel Ruttkowski Lukas Schwarz Kirsti Witter Waltraud Tschulenk Anja Joachim Isospora suis in an epithelial cell culture system - an in vitro model for sexual development in coccidia.
description	Coccidian parasites are of major importance in animal production, public health and food safety. The most frequently used representative in basic research on this group is Toxoplasma gondii. Although this parasite is well investigated there is no adequate in vitro model for its sexual development available and knowledge on this important life cycle phase is therefore scarce. The use of Isosporasuis, a sister taxon to T. gondii and the causative agent of piglet coccidiosis, could provide a solution for this. In the present study an in vitro model for neonatal porcine coccidiosis in cells representative for the in vivo situation in the piglet gut was developed and evaluated. The parasite development was investigated by light and transmission electron microscopy and optimum culture conditions were evaluated. Intestinal porcine epithelial cells (IPEC-J2) adequately representing the natural host cells supported the development of all endogenous life cycle stages of I. suis, including gametocytes and oocysts. A concentration of 5% fetal calf serum in the culture medium led to highest gametocyte densities on day 12 post infection. Low infection doses (≤1 sporozoite for 100 host cells) were best for oocyst and gametocyte development. The presented system can also be used for immunostaining with established antibodies developed against T. gondii (in our case, anti-TgIMC3 antibodies directed against the inner membrane complex 3). The complete life cycle of I. suis in a cell line representing the natural host cell type and species provides a unique model among coccidian parasites and can be used to address a wide range of topics, especially with regard to the sexual development of coccidia.
format	article
author	Hanna Lucia Worliczek Bärbel Ruttkowski Lukas Schwarz Kirsti Witter Waltraud Tschulenk Anja Joachim
author_facet	Hanna Lucia Worliczek Bärbel Ruttkowski Lukas Schwarz Kirsti Witter Waltraud Tschulenk Anja Joachim
author_sort	Hanna Lucia Worliczek
title	Isospora suis in an epithelial cell culture system - an in vitro model for sexual development in coccidia.
title_short	Isospora suis in an epithelial cell culture system - an in vitro model for sexual development in coccidia.
title_full	Isospora suis in an epithelial cell culture system - an in vitro model for sexual development in coccidia.
title_fullStr	Isospora suis in an epithelial cell culture system - an in vitro model for sexual development in coccidia.
title_full_unstemmed	Isospora suis in an epithelial cell culture system - an in vitro model for sexual development in coccidia.
title_sort	isospora suis in an epithelial cell culture system - an in vitro model for sexual development in coccidia.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e9130874406847148235826567fcda92
work_keys_str_mv	AT hannaluciaworliczek isosporasuisinanepithelialcellculturesystemaninvitromodelforsexualdevelopmentincoccidia AT barbelruttkowski isosporasuisinanepithelialcellculturesystemaninvitromodelforsexualdevelopmentincoccidia AT lukasschwarz isosporasuisinanepithelialcellculturesystemaninvitromodelforsexualdevelopmentincoccidia AT kirstiwitter isosporasuisinanepithelialcellculturesystemaninvitromodelforsexualdevelopmentincoccidia AT waltraudtschulenk isosporasuisinanepithelialcellculturesystemaninvitromodelforsexualdevelopmentincoccidia AT anjajoachim isosporasuisinanepithelialcellculturesystemaninvitromodelforsexualdevelopmentincoccidia
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Isospora suis in an epithelial cell culture system - an in vitro model for sexual development in coccidia.

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