Overexpressing STAMP2 improves insulin resistance in diabetic ApoE⁻/⁻/LDLR⁻/⁻ mice via macrophage polarization shift in adipose tissues.

STAMP2 is a counterregulator of inflammation and insulin resistance. The aim of this study is to investigate whether activation of STAMP2 improves insulin resistance by regulating macrophage polarization in adipose tissues. The diabetic ApoE⁻/⁻/LDLR⁻/⁻ mouse model was induced by high-fat diet and lo...

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Autores principales:	Lu Han, Meng-Xiong Tang, Yun Ti, Zhi-Hao Wang, Jia Wang, Wen-Yuan Ding, Hua Wang, Yun Zhang, Wei Zhang, Ming Zhong
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e92c32cc8b4648a0b88f28b3eb2e4887
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spelling	oai:doaj.org-article:e92c32cc8b4648a0b88f28b3eb2e48872021-11-18T08:46:52ZOverexpressing STAMP2 improves insulin resistance in diabetic ApoE⁻/⁻/LDLR⁻/⁻ mice via macrophage polarization shift in adipose tissues.1932-620310.1371/journal.pone.0078903https://doaj.org/article/e92c32cc8b4648a0b88f28b3eb2e48872013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24236066/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203STAMP2 is a counterregulator of inflammation and insulin resistance. The aim of this study is to investigate whether activation of STAMP2 improves insulin resistance by regulating macrophage polarization in adipose tissues. The diabetic ApoE⁻/⁻/LDLR⁻/⁻ mouse model was induced by high-fat diet and low-dose streptozotocin. Samples were obtained from epididymal, subcutaneous and brown adipose tissues. Infiltration of M1/M2 macrophages and inflammatory cytokines were investigated by immunohistochemistry. We then used gene overexpression to investigate the effect of STAMP2 on macrophages infiltration and polarization and inflammatory cytokines expression. Our results showed that infiltration of macrophages, the ratio of M1/M2 macrophages and the expression of pro-inflammatory cytokines were enhanced and STAMP2 was downregulated in adipose tissues of diabetic ApoE⁻/⁻/LDLR⁻/⁻ mice compared with control mice. STAMP2 gene overexpression could significantly reduce macrophages infiltration, the ratio of M1/M2 macrophages and the expression of pro-inflammatory cytokines in epididymal and brown adipose tissues, improving insulin resistance. Our results suggested that STAMP2 gene overexpression may improve insulin resistance via regulating macrophage polarization in visceral and brown adipose tissues.Lu HanMeng-Xiong TangYun TiZhi-Hao WangJia WangWen-Yuan DingHua WangYun ZhangWei ZhangMing ZhongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e78903 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lu Han Meng-Xiong Tang Yun Ti Zhi-Hao Wang Jia Wang Wen-Yuan Ding Hua Wang Yun Zhang Wei Zhang Ming Zhong Overexpressing STAMP2 improves insulin resistance in diabetic ApoE⁻/⁻/LDLR⁻/⁻ mice via macrophage polarization shift in adipose tissues.
description	STAMP2 is a counterregulator of inflammation and insulin resistance. The aim of this study is to investigate whether activation of STAMP2 improves insulin resistance by regulating macrophage polarization in adipose tissues. The diabetic ApoE⁻/⁻/LDLR⁻/⁻ mouse model was induced by high-fat diet and low-dose streptozotocin. Samples were obtained from epididymal, subcutaneous and brown adipose tissues. Infiltration of M1/M2 macrophages and inflammatory cytokines were investigated by immunohistochemistry. We then used gene overexpression to investigate the effect of STAMP2 on macrophages infiltration and polarization and inflammatory cytokines expression. Our results showed that infiltration of macrophages, the ratio of M1/M2 macrophages and the expression of pro-inflammatory cytokines were enhanced and STAMP2 was downregulated in adipose tissues of diabetic ApoE⁻/⁻/LDLR⁻/⁻ mice compared with control mice. STAMP2 gene overexpression could significantly reduce macrophages infiltration, the ratio of M1/M2 macrophages and the expression of pro-inflammatory cytokines in epididymal and brown adipose tissues, improving insulin resistance. Our results suggested that STAMP2 gene overexpression may improve insulin resistance via regulating macrophage polarization in visceral and brown adipose tissues.
format	article
author	Lu Han Meng-Xiong Tang Yun Ti Zhi-Hao Wang Jia Wang Wen-Yuan Ding Hua Wang Yun Zhang Wei Zhang Ming Zhong
author_facet	Lu Han Meng-Xiong Tang Yun Ti Zhi-Hao Wang Jia Wang Wen-Yuan Ding Hua Wang Yun Zhang Wei Zhang Ming Zhong
author_sort	Lu Han
title	Overexpressing STAMP2 improves insulin resistance in diabetic ApoE⁻/⁻/LDLR⁻/⁻ mice via macrophage polarization shift in adipose tissues.
title_short	Overexpressing STAMP2 improves insulin resistance in diabetic ApoE⁻/⁻/LDLR⁻/⁻ mice via macrophage polarization shift in adipose tissues.
title_full	Overexpressing STAMP2 improves insulin resistance in diabetic ApoE⁻/⁻/LDLR⁻/⁻ mice via macrophage polarization shift in adipose tissues.
title_fullStr	Overexpressing STAMP2 improves insulin resistance in diabetic ApoE⁻/⁻/LDLR⁻/⁻ mice via macrophage polarization shift in adipose tissues.
title_full_unstemmed	Overexpressing STAMP2 improves insulin resistance in diabetic ApoE⁻/⁻/LDLR⁻/⁻ mice via macrophage polarization shift in adipose tissues.
title_sort	overexpressing stamp2 improves insulin resistance in diabetic apoe⁻/⁻/ldlr⁻/⁻ mice via macrophage polarization shift in adipose tissues.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e92c32cc8b4648a0b88f28b3eb2e4887
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Overexpressing STAMP2 improves insulin resistance in diabetic ApoE⁻/⁻/LDLR⁻/⁻ mice via macrophage polarization shift in adipose tissues.

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