Assessment of Potential Cytotoxicity During Vital Observation at the BioStation CT

In recent decades, the methods based on the cell technologies have become more relevant for medical and pharmaceutical research. In this paper, weconsidered the BioStation CT using for continuous in vivo observation of Ehrlich ascitic carcinoma cell culture under the influence of two active substanc...

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Autores principales:	I. S. Trukhan, Т. N Dremina, E. A. Lozovskaya, I. A. Shurygina
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2019
Materias:	cytotoxicity co2-incubator biostation ct ehrlich carcinoma cells Science Q
Acceso en línea:	https://doaj.org/article/e9305394dc044c78bb63fee47dde2aaf
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spelling	oai:doaj.org-article:e9305394dc044c78bb63fee47dde2aaf2021-11-23T06:14:41ZAssessment of Potential Cytotoxicity During Vital Observation at the BioStation CT2541-94202587-959610.29413/ABS.2018-3.6.6https://doaj.org/article/e9305394dc044c78bb63fee47dde2aaf2019-01-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/1880https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596In recent decades, the methods based on the cell technologies have become more relevant for medical and pharmaceutical research. In this paper, weconsidered the BioStation CT using for continuous in vivo observation of Ehrlich ascitic carcinoma cell culture under the influence of two active substances in different concentrations (from 1.25 to 20 mg/l). As a result of the carcinoma cells condition monitoring for 4.5 days it was shown that substance No 2 does not affect the cell viability while substance No 1 causes the carcinoma cell death, and this active substance effect is dose-dependent. Photodocumentation at two-hour intervals allowed us to research differences in the rate of cell destruction (intensive disintegration in the first day with further stabilization of the living cells number or gradual cell death throughout the experiment), as well as differences in the time of 50 % mortality reaching. Thus in the study it was demonstrated that due to the fact that the BioStation CT combines the properties of a CO2 -incubator and a microscope this device is promising for toxicological studies and significantly expands the detection possibilities of processes occurring with living cells for a sufficiently long time period making possible further analysis of the cell behavior characteristics throughout the experiment, and that is fundamentally different from the systems allowing only the final result fixation of long-term active substance exposure.I. S. TrukhanТ. N DreminaE. A. LozovskayaI. A. ShuryginaScientific Сentre for Family Health and Human Reproduction Problemsarticlecytotoxicityco2-incubator biostation ctehrlich carcinoma cellsScienceQRUActa Biomedica Scientifica, Vol 3, Iss 6, Pp 48-53 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	cytotoxicity co2-incubator biostation ct ehrlich carcinoma cells Science Q
spellingShingle	cytotoxicity co2-incubator biostation ct ehrlich carcinoma cells Science Q I. S. Trukhan Т. N Dremina E. A. Lozovskaya I. A. Shurygina Assessment of Potential Cytotoxicity During Vital Observation at the BioStation CT
description	In recent decades, the methods based on the cell technologies have become more relevant for medical and pharmaceutical research. In this paper, weconsidered the BioStation CT using for continuous in vivo observation of Ehrlich ascitic carcinoma cell culture under the influence of two active substances in different concentrations (from 1.25 to 20 mg/l). As a result of the carcinoma cells condition monitoring for 4.5 days it was shown that substance No 2 does not affect the cell viability while substance No 1 causes the carcinoma cell death, and this active substance effect is dose-dependent. Photodocumentation at two-hour intervals allowed us to research differences in the rate of cell destruction (intensive disintegration in the first day with further stabilization of the living cells number or gradual cell death throughout the experiment), as well as differences in the time of 50 % mortality reaching. Thus in the study it was demonstrated that due to the fact that the BioStation CT combines the properties of a CO2 -incubator and a microscope this device is promising for toxicological studies and significantly expands the detection possibilities of processes occurring with living cells for a sufficiently long time period making possible further analysis of the cell behavior characteristics throughout the experiment, and that is fundamentally different from the systems allowing only the final result fixation of long-term active substance exposure.
format	article
author	I. S. Trukhan Т. N Dremina E. A. Lozovskaya I. A. Shurygina
author_facet	I. S. Trukhan Т. N Dremina E. A. Lozovskaya I. A. Shurygina
author_sort	I. S. Trukhan
title	Assessment of Potential Cytotoxicity During Vital Observation at the BioStation CT
title_short	Assessment of Potential Cytotoxicity During Vital Observation at the BioStation CT
title_full	Assessment of Potential Cytotoxicity During Vital Observation at the BioStation CT
title_fullStr	Assessment of Potential Cytotoxicity During Vital Observation at the BioStation CT
title_full_unstemmed	Assessment of Potential Cytotoxicity During Vital Observation at the BioStation CT
title_sort	assessment of potential cytotoxicity during vital observation at the biostation ct
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2019
url	https://doaj.org/article/e9305394dc044c78bb63fee47dde2aaf
work_keys_str_mv	AT istrukhan assessmentofpotentialcytotoxicityduringvitalobservationatthebiostationct AT tndremina assessmentofpotentialcytotoxicityduringvitalobservationatthebiostationct AT ealozovskaya assessmentofpotentialcytotoxicityduringvitalobservationatthebiostationct AT iashurygina assessmentofpotentialcytotoxicityduringvitalobservationatthebiostationct
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Assessment of Potential Cytotoxicity During Vital Observation at the BioStation CT

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