Inheritance of chloroplast and mitochondrial genomes in cucumber revealed by four reciprocal F1 hybrid combinations

Abstract Both genomes in chloroplasts and mitochondria of plant cell are usually inherited from maternal parent, with rare exceptions. To characterize the inheritance patterns of the organelle genomes in cucumber (Cucumis sativus var. sativus), two inbred lines and their reciprocal F1 hybrids were a...

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Autores principales:	Hyun-Seung Park, Won Kyung Lee, Sang-Choon Lee, Hyun Oh Lee, Ho Jun Joh, Jee Young Park, Sunggil Kim, Kihwan Song, Tae-Jin Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e931b02dddbc42ec89407cd1756c1534
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spelling	oai:doaj.org-article:e931b02dddbc42ec89407cd1756c15342021-12-02T14:16:57ZInheritance of chloroplast and mitochondrial genomes in cucumber revealed by four reciprocal F1 hybrid combinations10.1038/s41598-021-81988-w2045-2322https://doaj.org/article/e931b02dddbc42ec89407cd1756c15342021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81988-whttps://doaj.org/toc/2045-2322Abstract Both genomes in chloroplasts and mitochondria of plant cell are usually inherited from maternal parent, with rare exceptions. To characterize the inheritance patterns of the organelle genomes in cucumber (Cucumis sativus var. sativus), two inbred lines and their reciprocal F1 hybrids were analyzed using an next generation whole genome sequencing data. Their complete chloroplast genome sequences were de novo assembled, and a single SNP was identified between the parental lines. Two reciprocal F1 hybrids have the same chloroplast genomes with their maternal parents. Meanwhile, 292 polymorphic sites were identified between mitochondrial genomes of the two parental lines, which showed the same genotypes with their paternal parents in the two reciprocal F1 hybrids, without any recombination. The inheritance patterns of the chloroplast and mitochondria genomes were also confirmed in four additional cucumber accessions and their six reciprocal F1 hybrids using molecular markers derived from the identified polymorphic sites. Taken together, our results indicate that the cucumber chloroplast genome is maternally inherited, as is typically observed in other plant species, whereas the large cucumber mitochondrial genome is paternally inherited. The combination of DNA markers derived from the chloroplast and mitochondrial genomes will provide a convenient system for purity test of F1 hybrid seeds in cucumber breeding.Hyun-Seung ParkWon Kyung LeeSang-Choon LeeHyun Oh LeeHo Jun JohJee Young ParkSunggil KimKihwan SongTae-Jin YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hyun-Seung Park Won Kyung Lee Sang-Choon Lee Hyun Oh Lee Ho Jun Joh Jee Young Park Sunggil Kim Kihwan Song Tae-Jin Yang Inheritance of chloroplast and mitochondrial genomes in cucumber revealed by four reciprocal F1 hybrid combinations
description	Abstract Both genomes in chloroplasts and mitochondria of plant cell are usually inherited from maternal parent, with rare exceptions. To characterize the inheritance patterns of the organelle genomes in cucumber (Cucumis sativus var. sativus), two inbred lines and their reciprocal F1 hybrids were analyzed using an next generation whole genome sequencing data. Their complete chloroplast genome sequences were de novo assembled, and a single SNP was identified between the parental lines. Two reciprocal F1 hybrids have the same chloroplast genomes with their maternal parents. Meanwhile, 292 polymorphic sites were identified between mitochondrial genomes of the two parental lines, which showed the same genotypes with their paternal parents in the two reciprocal F1 hybrids, without any recombination. The inheritance patterns of the chloroplast and mitochondria genomes were also confirmed in four additional cucumber accessions and their six reciprocal F1 hybrids using molecular markers derived from the identified polymorphic sites. Taken together, our results indicate that the cucumber chloroplast genome is maternally inherited, as is typically observed in other plant species, whereas the large cucumber mitochondrial genome is paternally inherited. The combination of DNA markers derived from the chloroplast and mitochondrial genomes will provide a convenient system for purity test of F1 hybrid seeds in cucumber breeding.
format	article
author	Hyun-Seung Park Won Kyung Lee Sang-Choon Lee Hyun Oh Lee Ho Jun Joh Jee Young Park Sunggil Kim Kihwan Song Tae-Jin Yang
author_facet	Hyun-Seung Park Won Kyung Lee Sang-Choon Lee Hyun Oh Lee Ho Jun Joh Jee Young Park Sunggil Kim Kihwan Song Tae-Jin Yang
author_sort	Hyun-Seung Park
title	Inheritance of chloroplast and mitochondrial genomes in cucumber revealed by four reciprocal F1 hybrid combinations
title_short	Inheritance of chloroplast and mitochondrial genomes in cucumber revealed by four reciprocal F1 hybrid combinations
title_full	Inheritance of chloroplast and mitochondrial genomes in cucumber revealed by four reciprocal F1 hybrid combinations
title_fullStr	Inheritance of chloroplast and mitochondrial genomes in cucumber revealed by four reciprocal F1 hybrid combinations
title_full_unstemmed	Inheritance of chloroplast and mitochondrial genomes in cucumber revealed by four reciprocal F1 hybrid combinations
title_sort	inheritance of chloroplast and mitochondrial genomes in cucumber revealed by four reciprocal f1 hybrid combinations
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e931b02dddbc42ec89407cd1756c1534
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Inheritance of chloroplast and mitochondrial genomes in cucumber revealed by four reciprocal F1 hybrid combinations

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