Multi-Omics Reveals the Inhibition of <i>Lactiplantibacillus plantarum</i> CCFM8724 in <i>Streptococcus mutans</i>-<i>Candida albicans</i> Mixed-Species Biofilms
<i>Lactiplantibacillus plantarum</i> CCFM8724 is a probiotic with the potential to prevent dental caries in vitro and in vivo. To explore the effects of this probiotic at inhibiting <i>Streptococcus mutans</i>-<i>Candida albicans</i> mixed-species biofilm and prev...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/e932391ea1af4e83b6281a930d192ee0 |
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| Sumario: | <i>Lactiplantibacillus plantarum</i> CCFM8724 is a probiotic with the potential to prevent dental caries in vitro and in vivo. To explore the effects of this probiotic at inhibiting <i>Streptococcus mutans</i>-<i>Candida albicans</i> mixed-species biofilm and preventing dental caries, multi-omics, including metabolomics and transcriptomics, was used to investigate the regulation of small-molecule metabolism during biofilm formation and the gene expression in the mixed-species biofilm. Metabolomic analysis revealed that some carbohydrates related to biofilm formation, such as sucrose, was detected at lower levels due to the treatment with the <i>L. plantarum</i> supernatant. Some sugar alcohols, such as xylitol and sorbitol, were detected at higher levels, which may have inhibited the growth of <i>S. mutans</i>. In transcriptomic analysis, the expression of the virulence genes of <i>C. albicans</i>, such as those that code agglutinin-like sequence (<i>Als</i>) proteins, was affected. In addition, metabolomics coupled with a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and RNA-seq revealed that the <i>L. plantarum</i> supernatant had an active role in sugar metabolism during the formation of the <i>S. mutans</i>-<i>C. albicans</i> mixed-species biofilm, and the <i>L. plantarum</i> supernatant was also related to carbohydrate utilization, glucan biosynthesis, and mycelium formation. Hence, <i>L. plantarum</i> CCFM8724 decreased the mixed-species biofilm mass from the perspective of gene expression and metabolic reprogramming. Our results provide a rationale for evaluating <i>L. plantarum</i> CCFM8724 as a potential oral probiotic for inhibiting cariogenic pathogen biofilm formation and improving dental caries. |
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