Multi-Omics Reveals the Inhibition of Lactiplantibacillus plantarum CCFM8724 in Streptococcus mutans-Candida albicans Mixed-Species Biofilms

Lactiplantibacillus plantarum CCFM8724 is a probiotic with the potential to prevent dental caries in vitro and in vivo. To explore the effects of this probiotic at inhibiting Streptococcus mutans-Candida albicans mixed-species biofilm and prev...

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Autores principales:	Qiuxiang Zhang, Jiaxun Li, Wenwei Lu, Jianxin Zhao, Hao Zhang, Wei Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Streptococcus mutans</i> <i>Candida albicans</i> transcriptomics metabolomics biofilm dental caries Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e932391ea1af4e83b6281a930d192ee0
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spelling	oai:doaj.org-article:e932391ea1af4e83b6281a930d192ee02021-11-25T18:25:28ZMulti-Omics Reveals the Inhibition of <i>Lactiplantibacillus plantarum</i> CCFM8724 in <i>Streptococcus mutans</i>-<i>Candida albicans</i> Mixed-Species Biofilms10.3390/microorganisms91123682076-2607https://doaj.org/article/e932391ea1af4e83b6281a930d192ee02021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2368https://doaj.org/toc/2076-2607<i>Lactiplantibacillus plantarum</i> CCFM8724 is a probiotic with the potential to prevent dental caries in vitro and in vivo. To explore the effects of this probiotic at inhibiting <i>Streptococcus mutans</i>-<i>Candida albicans</i> mixed-species biofilm and preventing dental caries, multi-omics, including metabolomics and transcriptomics, was used to investigate the regulation of small-molecule metabolism during biofilm formation and the gene expression in the mixed-species biofilm. Metabolomic analysis revealed that some carbohydrates related to biofilm formation, such as sucrose, was detected at lower levels due to the treatment with the <i>L. plantarum</i> supernatant. Some sugar alcohols, such as xylitol and sorbitol, were detected at higher levels, which may have inhibited the growth of <i>S. mutans</i>. In transcriptomic analysis, the expression of the virulence genes of <i>C. albicans</i>, such as those that code agglutinin-like sequence (<i>Als</i>) proteins, was affected. In addition, metabolomics coupled with a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and RNA-seq revealed that the <i>L. plantarum</i> supernatant had an active role in sugar metabolism during the formation of the <i>S. mutans</i>-<i>C. albicans</i> mixed-species biofilm, and the <i>L. plantarum</i> supernatant was also related to carbohydrate utilization, glucan biosynthesis, and mycelium formation. Hence, <i>L. plantarum</i> CCFM8724 decreased the mixed-species biofilm mass from the perspective of gene expression and metabolic reprogramming. Our results provide a rationale for evaluating <i>L. plantarum</i> CCFM8724 as a potential oral probiotic for inhibiting cariogenic pathogen biofilm formation and improving dental caries.Qiuxiang ZhangJiaxun LiWenwei LuJianxin ZhaoHao ZhangWei ChenMDPI AGarticle<i>Streptococcus mutans</i><i>Candida albicans</i>transcriptomicsmetabolomicsbiofilmdental cariesBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2368, p 2368 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Streptococcus mutans</i> <i>Candida albicans</i> transcriptomics metabolomics biofilm dental caries Biology (General) QH301-705.5
spellingShingle	<i>Streptococcus mutans</i> <i>Candida albicans</i> transcriptomics metabolomics biofilm dental caries Biology (General) QH301-705.5 Qiuxiang Zhang Jiaxun Li Wenwei Lu Jianxin Zhao Hao Zhang Wei Chen Multi-Omics Reveals the Inhibition of <i>Lactiplantibacillus plantarum</i> CCFM8724 in <i>Streptococcus mutans</i>-<i>Candida albicans</i> Mixed-Species Biofilms
description	<i>Lactiplantibacillus plantarum</i> CCFM8724 is a probiotic with the potential to prevent dental caries in vitro and in vivo. To explore the effects of this probiotic at inhibiting <i>Streptococcus mutans</i>-<i>Candida albicans</i> mixed-species biofilm and preventing dental caries, multi-omics, including metabolomics and transcriptomics, was used to investigate the regulation of small-molecule metabolism during biofilm formation and the gene expression in the mixed-species biofilm. Metabolomic analysis revealed that some carbohydrates related to biofilm formation, such as sucrose, was detected at lower levels due to the treatment with the <i>L. plantarum</i> supernatant. Some sugar alcohols, such as xylitol and sorbitol, were detected at higher levels, which may have inhibited the growth of <i>S. mutans</i>. In transcriptomic analysis, the expression of the virulence genes of <i>C. albicans</i>, such as those that code agglutinin-like sequence (<i>Als</i>) proteins, was affected. In addition, metabolomics coupled with a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and RNA-seq revealed that the <i>L. plantarum</i> supernatant had an active role in sugar metabolism during the formation of the <i>S. mutans</i>-<i>C. albicans</i> mixed-species biofilm, and the <i>L. plantarum</i> supernatant was also related to carbohydrate utilization, glucan biosynthesis, and mycelium formation. Hence, <i>L. plantarum</i> CCFM8724 decreased the mixed-species biofilm mass from the perspective of gene expression and metabolic reprogramming. Our results provide a rationale for evaluating <i>L. plantarum</i> CCFM8724 as a potential oral probiotic for inhibiting cariogenic pathogen biofilm formation and improving dental caries.
format	article
author	Qiuxiang Zhang Jiaxun Li Wenwei Lu Jianxin Zhao Hao Zhang Wei Chen
author_facet	Qiuxiang Zhang Jiaxun Li Wenwei Lu Jianxin Zhao Hao Zhang Wei Chen
author_sort	Qiuxiang Zhang
title	Multi-Omics Reveals the Inhibition of <i>Lactiplantibacillus plantarum</i> CCFM8724 in <i>Streptococcus mutans</i>-<i>Candida albicans</i> Mixed-Species Biofilms
title_short	Multi-Omics Reveals the Inhibition of <i>Lactiplantibacillus plantarum</i> CCFM8724 in <i>Streptococcus mutans</i>-<i>Candida albicans</i> Mixed-Species Biofilms
title_full	Multi-Omics Reveals the Inhibition of <i>Lactiplantibacillus plantarum</i> CCFM8724 in <i>Streptococcus mutans</i>-<i>Candida albicans</i> Mixed-Species Biofilms
title_fullStr	Multi-Omics Reveals the Inhibition of <i>Lactiplantibacillus plantarum</i> CCFM8724 in <i>Streptococcus mutans</i>-<i>Candida albicans</i> Mixed-Species Biofilms
title_full_unstemmed	Multi-Omics Reveals the Inhibition of <i>Lactiplantibacillus plantarum</i> CCFM8724 in <i>Streptococcus mutans</i>-<i>Candida albicans</i> Mixed-Species Biofilms
title_sort	multi-omics reveals the inhibition of <i>lactiplantibacillus plantarum</i> ccfm8724 in <i>streptococcus mutans</i>-<i>candida albicans</i> mixed-species biofilms
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e932391ea1af4e83b6281a930d192ee0
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Multi-Omics Reveals the Inhibition of <i>Lactiplantibacillus plantarum</i> CCFM8724 in <i>Streptococcus mutans</i>-<i>Candida albicans</i> Mixed-Species Biofilms

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