Impact of synbiotics on gut microbiota during early life: a randomized, double-blind study

Abstract Human milk is considered the optimal nutrition for infants and found to contain significant numbers of viable bacteria. The aim of the study was to assess the effects of a specific synbiotic combination at doses closer to the bacterial cells present in human milk, on intestinal bifidobacter...

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Autores principales: Nopaorn Phavichitr, Shugui Wang, Sirinuch Chomto, Ruangvith Tantibhaedhyangkul, Alexia Kakourou, Sukkrawan Intarakhao, Sungkom Jongpiputvanich, COLOR Study Group, Guus Roeselers, Jan Knol
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/e935cad5b39f45c4bb79232e5d535bc9
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spelling oai:doaj.org-article:e935cad5b39f45c4bb79232e5d535bc92021-12-02T14:26:54ZImpact of synbiotics on gut microbiota during early life: a randomized, double-blind study10.1038/s41598-021-83009-22045-2322https://doaj.org/article/e935cad5b39f45c4bb79232e5d535bc92021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83009-2https://doaj.org/toc/2045-2322Abstract Human milk is considered the optimal nutrition for infants and found to contain significant numbers of viable bacteria. The aim of the study was to assess the effects of a specific synbiotic combination at doses closer to the bacterial cells present in human milk, on intestinal bifidobacteria proportions (relative abundance), reduction of potential pathogens and gut physiological conditions. A clinical study was conducted in 290 healthy infants aged from 6 to 19 weeks. Infants received either a control infant formula or one of the two investigational infant formulas (control formula with 0.8 g/100 ml scGOS/lcFOS and Bifidobacterium breve M-16V at either 1 × 104 cfu/ml or 1 × 106 cfu/ml). Exclusively breastfed infants were included as a reference. Analyses were performed on intention-to-treat groups and all-subjects-treated groups. After 6 weeks of intervention, the synbiotics at two different doses significantly increased the bifidobacteria proportions in healthy infants. The synbiotic supplementation also decreased the prevalence (infants with detectable levels) and the abundance of C. difficile. Closer to the levels in the breastfed reference group, fecal pH was significantly lower while l-lactate concentrations and acetate proportions were significantly higher in the synbiotic groups. All formulas were well tolerated and all groups showed a comparable safety profile based on the number and severity of adverse events and growth. In healthy infants, supplementation of infant-type bifidobacterial strain B. breve M-16V, at a dose close to bacterial numbers found in human milk, with scGOS/lcFOS (9:1) created a gut environment closer to the breastfed reference group. This specific synbiotic mixture may also support gut microbiota resilience during early life. Clinical Trial Registration This clinical study named Color Synbiotics Study, was registered in ClinicalTrials.gov on 18 March 2013. Registration number is NCT01813175. https://clinicaltrials.gov/ct2/show/NCT01813175 .Nopaorn PhavichitrShugui WangSirinuch ChomtoRuangvith TantibhaedhyangkulAlexia KakourouSukkrawan IntarakhaoSungkom JongpiputvanichCOLOR Study GroupGuus RoeselersJan KnolNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Nopaorn Phavichitr
Shugui Wang
Sirinuch Chomto
Ruangvith Tantibhaedhyangkul
Alexia Kakourou
Sukkrawan Intarakhao
Sungkom Jongpiputvanich
COLOR Study Group
Guus Roeselers
Jan Knol
Impact of synbiotics on gut microbiota during early life: a randomized, double-blind study
description Abstract Human milk is considered the optimal nutrition for infants and found to contain significant numbers of viable bacteria. The aim of the study was to assess the effects of a specific synbiotic combination at doses closer to the bacterial cells present in human milk, on intestinal bifidobacteria proportions (relative abundance), reduction of potential pathogens and gut physiological conditions. A clinical study was conducted in 290 healthy infants aged from 6 to 19 weeks. Infants received either a control infant formula or one of the two investigational infant formulas (control formula with 0.8 g/100 ml scGOS/lcFOS and Bifidobacterium breve M-16V at either 1 × 104 cfu/ml or 1 × 106 cfu/ml). Exclusively breastfed infants were included as a reference. Analyses were performed on intention-to-treat groups and all-subjects-treated groups. After 6 weeks of intervention, the synbiotics at two different doses significantly increased the bifidobacteria proportions in healthy infants. The synbiotic supplementation also decreased the prevalence (infants with detectable levels) and the abundance of C. difficile. Closer to the levels in the breastfed reference group, fecal pH was significantly lower while l-lactate concentrations and acetate proportions were significantly higher in the synbiotic groups. All formulas were well tolerated and all groups showed a comparable safety profile based on the number and severity of adverse events and growth. In healthy infants, supplementation of infant-type bifidobacterial strain B. breve M-16V, at a dose close to bacterial numbers found in human milk, with scGOS/lcFOS (9:1) created a gut environment closer to the breastfed reference group. This specific synbiotic mixture may also support gut microbiota resilience during early life. Clinical Trial Registration This clinical study named Color Synbiotics Study, was registered in ClinicalTrials.gov on 18 March 2013. Registration number is NCT01813175. https://clinicaltrials.gov/ct2/show/NCT01813175 .
format article
author Nopaorn Phavichitr
Shugui Wang
Sirinuch Chomto
Ruangvith Tantibhaedhyangkul
Alexia Kakourou
Sukkrawan Intarakhao
Sungkom Jongpiputvanich
COLOR Study Group
Guus Roeselers
Jan Knol
author_facet Nopaorn Phavichitr
Shugui Wang
Sirinuch Chomto
Ruangvith Tantibhaedhyangkul
Alexia Kakourou
Sukkrawan Intarakhao
Sungkom Jongpiputvanich
COLOR Study Group
Guus Roeselers
Jan Knol
author_sort Nopaorn Phavichitr
title Impact of synbiotics on gut microbiota during early life: a randomized, double-blind study
title_short Impact of synbiotics on gut microbiota during early life: a randomized, double-blind study
title_full Impact of synbiotics on gut microbiota during early life: a randomized, double-blind study
title_fullStr Impact of synbiotics on gut microbiota during early life: a randomized, double-blind study
title_full_unstemmed Impact of synbiotics on gut microbiota during early life: a randomized, double-blind study
title_sort impact of synbiotics on gut microbiota during early life: a randomized, double-blind study
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/e935cad5b39f45c4bb79232e5d535bc9
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