mSphere of Influence: Organoids and Single-Cell Sequencing, a Powerful Combination To Uncover Epithelial and Immune Cell Interactions in the Human Gut Environment

ABSTRACT Jose Lemme-Dumit works in the field of mucosal immunology and vaccines. In this mSphere of Influence article, he reflects on how two papers, “Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett’s epithelium” by Sato et al. (T. Sato, D. E. Stang...

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Autor principal:	Jose M. Lemme-Dumit
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	enteroid immune cells mucosal immunology single-cell sequencing Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e9378cf2b8c2490f80b7f202e547acbd
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spelling	oai:doaj.org-article:e9378cf2b8c2490f80b7f202e547acbd2021-11-15T15:30:52ZmSphere of Influence: Organoids and Single-Cell Sequencing, a Powerful Combination To Uncover Epithelial and Immune Cell Interactions in the Human Gut Environment10.1128/mSphere.00722-202379-5042https://doaj.org/article/e9378cf2b8c2490f80b7f202e547acbd2020-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00722-20https://doaj.org/toc/2379-5042ABSTRACT Jose Lemme-Dumit works in the field of mucosal immunology and vaccines. In this mSphere of Influence article, he reflects on how two papers, “Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett’s epithelium” by Sato et al. (T. Sato, D. E. Stange, M. Ferrante, R. G. J. Vries, et al., Gastroenterology 141:1762–1772, 2011, https://doi.org/10.1053/j.gastro.2011.07.050) and “T helper cell cytokines modulate intestinal stem cell renewal and differentiation” by Biton et al. (M. Biton, A. L. Haber, N. Rogel, G. Burgin, et al., Cell 175:1307–1320.E22, 2018, https://doi.org/10.1016/j.cell.2018.10.008), have influenced his research by describing the development of intestinal organoid cultures and implementation of high-throughput sequencing analysis. The combination of these forefront technologies has expanded opportunities for mechanistic interrogation of host immunity to enteric pathogens.Jose M. Lemme-DumitAmerican Society for Microbiologyarticleenteroidimmune cellsmucosal immunologysingle-cell sequencingMicrobiologyQR1-502ENmSphere, Vol 5, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	enteroid immune cells mucosal immunology single-cell sequencing Microbiology QR1-502
spellingShingle	enteroid immune cells mucosal immunology single-cell sequencing Microbiology QR1-502 Jose M. Lemme-Dumit mSphere of Influence: Organoids and Single-Cell Sequencing, a Powerful Combination To Uncover Epithelial and Immune Cell Interactions in the Human Gut Environment
description	ABSTRACT Jose Lemme-Dumit works in the field of mucosal immunology and vaccines. In this mSphere of Influence article, he reflects on how two papers, “Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett’s epithelium” by Sato et al. (T. Sato, D. E. Stange, M. Ferrante, R. G. J. Vries, et al., Gastroenterology 141:1762–1772, 2011, https://doi.org/10.1053/j.gastro.2011.07.050) and “T helper cell cytokines modulate intestinal stem cell renewal and differentiation” by Biton et al. (M. Biton, A. L. Haber, N. Rogel, G. Burgin, et al., Cell 175:1307–1320.E22, 2018, https://doi.org/10.1016/j.cell.2018.10.008), have influenced his research by describing the development of intestinal organoid cultures and implementation of high-throughput sequencing analysis. The combination of these forefront technologies has expanded opportunities for mechanistic interrogation of host immunity to enteric pathogens.
format	article
author	Jose M. Lemme-Dumit
author_facet	Jose M. Lemme-Dumit
author_sort	Jose M. Lemme-Dumit
title	mSphere of Influence: Organoids and Single-Cell Sequencing, a Powerful Combination To Uncover Epithelial and Immune Cell Interactions in the Human Gut Environment
title_short	mSphere of Influence: Organoids and Single-Cell Sequencing, a Powerful Combination To Uncover Epithelial and Immune Cell Interactions in the Human Gut Environment
title_full	mSphere of Influence: Organoids and Single-Cell Sequencing, a Powerful Combination To Uncover Epithelial and Immune Cell Interactions in the Human Gut Environment
title_fullStr	mSphere of Influence: Organoids and Single-Cell Sequencing, a Powerful Combination To Uncover Epithelial and Immune Cell Interactions in the Human Gut Environment
title_full_unstemmed	mSphere of Influence: Organoids and Single-Cell Sequencing, a Powerful Combination To Uncover Epithelial and Immune Cell Interactions in the Human Gut Environment
title_sort	msphere of influence: organoids and single-cell sequencing, a powerful combination to uncover epithelial and immune cell interactions in the human gut environment
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/e9378cf2b8c2490f80b7f202e547acbd
work_keys_str_mv	AT josemlemmedumit msphereofinfluenceorganoidsandsinglecellsequencingapowerfulcombinationtouncoverepithelialandimmunecellinteractionsinthehumangutenvironment
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mSphere of Influence: Organoids and Single-Cell Sequencing, a Powerful Combination To Uncover Epithelial and Immune Cell Interactions in the Human Gut Environment

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