Usability of abattoir-acquired pig eyes for refractive excimer laser research

Usability of abattoir-acquired pig eyes for refractive excimer laser research

Abstract The purpose of this study was to elucidate, under which conditions abattoir-acquired pig eyes are suitable for refractive excimer laser experiments. Porcine eyes from tunnel-scalded (n = 5) and tank-scalded (n = 10) pigs were compared to unscalded eyes (n = 5) and to eyes scalded in the lab...

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Autores principales: Marius Topka, Yao Zhang, Antonia Bock, Peter Riedel, Johannes Lörner, Alexander Hammer, Eva Maier, Friedrich Paulsen, Christian M. Hammer
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Science
Q
Acceso en línea:https://doaj.org/article/e94fa0753ace4f838e50172a4c84943d
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spelling oai:doaj.org-article:e94fa0753ace4f838e50172a4c84943d2021-12-02T19:17:05ZUsability of abattoir-acquired pig eyes for refractive excimer laser research10.1038/s41598-021-98635-z2045-2322https://doaj.org/article/e94fa0753ace4f838e50172a4c84943d2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98635-zhttps://doaj.org/toc/2045-2322Abstract The purpose of this study was to elucidate, under which conditions abattoir-acquired pig eyes are suitable for refractive excimer laser experiments. Porcine eyes from tunnel-scalded (n = 5) and tank-scalded (n = 10) pigs were compared to unscalded eyes (n = 5) and to eyes scalded in the laboratory (n = 5). The corneal epithelium was removed before an excimer laser was used to perform a − 8.0 D photoablation. Corneal thickness was measured by optical coherence topography before and after photoablation. The ablation depth was determined with a contour measuring station, the morphology of the ablated areas was characterized by scanning electron microscopy and white-light profilometry. The scalded eyes showed an increase in corneal swelling which gained statistical significance in tank-scalded eyes showing a wedge-shaped opaque stromal lesion in the nasal corneal quadrant. A measurable deterioration of photoablation was only found in tank-scalded eyes that exhibited the opaque lesion. Ablated area morphology was smooth and regular in the unscalded and tunnel-scalded eyes. The tank-scalded eyes showed conspicuous wrinkles. While unscalded eyes should always be preferred for excimer laser laboratory experiments, the data suggest that the use of tunnel-scalded eyes may also be acceptable and should be chosen over tank-scalded eyes.Marius TopkaYao ZhangAntonia BockPeter RiedelJohannes LörnerAlexander HammerEva MaierFriedrich PaulsenChristian M. HammerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Marius Topka
Yao Zhang
Antonia Bock
Peter Riedel
Johannes Lörner
Alexander Hammer
Eva Maier
Friedrich Paulsen
Christian M. Hammer
Usability of abattoir-acquired pig eyes for refractive excimer laser research
description Abstract The purpose of this study was to elucidate, under which conditions abattoir-acquired pig eyes are suitable for refractive excimer laser experiments. Porcine eyes from tunnel-scalded (n = 5) and tank-scalded (n = 10) pigs were compared to unscalded eyes (n = 5) and to eyes scalded in the laboratory (n = 5). The corneal epithelium was removed before an excimer laser was used to perform a − 8.0 D photoablation. Corneal thickness was measured by optical coherence topography before and after photoablation. The ablation depth was determined with a contour measuring station, the morphology of the ablated areas was characterized by scanning electron microscopy and white-light profilometry. The scalded eyes showed an increase in corneal swelling which gained statistical significance in tank-scalded eyes showing a wedge-shaped opaque stromal lesion in the nasal corneal quadrant. A measurable deterioration of photoablation was only found in tank-scalded eyes that exhibited the opaque lesion. Ablated area morphology was smooth and regular in the unscalded and tunnel-scalded eyes. The tank-scalded eyes showed conspicuous wrinkles. While unscalded eyes should always be preferred for excimer laser laboratory experiments, the data suggest that the use of tunnel-scalded eyes may also be acceptable and should be chosen over tank-scalded eyes.
format article
author Marius Topka
Yao Zhang
Antonia Bock
Peter Riedel
Johannes Lörner
Alexander Hammer
Eva Maier
Friedrich Paulsen
Christian M. Hammer
author_facet Marius Topka
Yao Zhang
Antonia Bock
Peter Riedel
Johannes Lörner
Alexander Hammer
Eva Maier
Friedrich Paulsen
Christian M. Hammer
author_sort Marius Topka
title Usability of abattoir-acquired pig eyes for refractive excimer laser research
title_short Usability of abattoir-acquired pig eyes for refractive excimer laser research
title_full Usability of abattoir-acquired pig eyes for refractive excimer laser research
title_fullStr Usability of abattoir-acquired pig eyes for refractive excimer laser research
title_full_unstemmed Usability of abattoir-acquired pig eyes for refractive excimer laser research
title_sort usability of abattoir-acquired pig eyes for refractive excimer laser research
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/e94fa0753ace4f838e50172a4c84943d
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