Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane

Abstract Modulating biomolecular networks in cells with peptides and proteins has become a promising therapeutic strategy and effective biological tools. A simple and effective reagent that can bring functional proteins into cells can increase efficacy and allow more investigations. Here we show tha...

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Autores principales:	Kittima Amornwachirabodee, Nattapol Tantimekin, Porntip Pan-In, Tanapat Palaga, Prompong Pienpinijtham, Chonlatip Pipattanaboon, Thanyada Sukmanee, Patcharee Ritprajak, Promchat Charoenpat, Pannamthip Pitaksajjakul, Pongrama Ramasoota, Supason Wanichwecharungruang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e95249bfc79b41049602cc37c7a66f0a
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spelling	oai:doaj.org-article:e95249bfc79b41049602cc37c7a66f0a2021-12-02T15:08:29ZOxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane10.1038/s41598-018-20650-42045-2322https://doaj.org/article/e95249bfc79b41049602cc37c7a66f0a2018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-20650-4https://doaj.org/toc/2045-2322Abstract Modulating biomolecular networks in cells with peptides and proteins has become a promising therapeutic strategy and effective biological tools. A simple and effective reagent that can bring functional proteins into cells can increase efficacy and allow more investigations. Here we show that the relatively non-toxic and non-immunogenic oxidized carbon black particles (OCBs) prepared from commercially available carbon black can deliver a 300 kDa protein directly into cells, without an involvement of a cellular endocytosis. Experiments with cell-sized liposomes indicate that OCBs directly interact with phospholipids and induce membrane leakages. Delivery of human monoclonal antibodies (HuMAbs, 150 kDa) with specific affinity towards dengue viruses (DENV) into DENV-infected Vero cells by OCBs results in HuMAbs distribution all over cells’ interior and effective viral neutralization. An ability of OCBs to deliver big functional/therapeutic proteins into cells should open doors for more protein drug investigations and new levels of antibody therapies and biological studies.Kittima AmornwachirabodeeNattapol TantimekinPorntip Pan-InTanapat PalagaPrompong PienpinijthamChonlatip PipattanaboonThanyada SukmaneePatcharee RitprajakPromchat CharoenpatPannamthip PitaksajjakulPongrama RamasootaSupason WanichwecharungruangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kittima Amornwachirabodee Nattapol Tantimekin Porntip Pan-In Tanapat Palaga Prompong Pienpinijtham Chonlatip Pipattanaboon Thanyada Sukmanee Patcharee Ritprajak Promchat Charoenpat Pannamthip Pitaksajjakul Pongrama Ramasoota Supason Wanichwecharungruang Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane
description	Abstract Modulating biomolecular networks in cells with peptides and proteins has become a promising therapeutic strategy and effective biological tools. A simple and effective reagent that can bring functional proteins into cells can increase efficacy and allow more investigations. Here we show that the relatively non-toxic and non-immunogenic oxidized carbon black particles (OCBs) prepared from commercially available carbon black can deliver a 300 kDa protein directly into cells, without an involvement of a cellular endocytosis. Experiments with cell-sized liposomes indicate that OCBs directly interact with phospholipids and induce membrane leakages. Delivery of human monoclonal antibodies (HuMAbs, 150 kDa) with specific affinity towards dengue viruses (DENV) into DENV-infected Vero cells by OCBs results in HuMAbs distribution all over cells’ interior and effective viral neutralization. An ability of OCBs to deliver big functional/therapeutic proteins into cells should open doors for more protein drug investigations and new levels of antibody therapies and biological studies.
format	article
author	Kittima Amornwachirabodee Nattapol Tantimekin Porntip Pan-In Tanapat Palaga Prompong Pienpinijtham Chonlatip Pipattanaboon Thanyada Sukmanee Patcharee Ritprajak Promchat Charoenpat Pannamthip Pitaksajjakul Pongrama Ramasoota Supason Wanichwecharungruang
author_facet	Kittima Amornwachirabodee Nattapol Tantimekin Porntip Pan-In Tanapat Palaga Prompong Pienpinijtham Chonlatip Pipattanaboon Thanyada Sukmanee Patcharee Ritprajak Promchat Charoenpat Pannamthip Pitaksajjakul Pongrama Ramasoota Supason Wanichwecharungruang
author_sort	Kittima Amornwachirabodee
title	Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane
title_short	Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane
title_full	Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane
title_fullStr	Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane
title_full_unstemmed	Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane
title_sort	oxidized carbon black: preparation, characterization and application in antibody delivery across cell membrane
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/e95249bfc79b41049602cc37c7a66f0a
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Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane

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