Evaluation of the Effect of Inactivated Transmissible Gastroenteritis Virus Vaccine with Nano Silicon on the Phenotype and Function of Porcine Dendritic Cells

A transmissible gastroenteritis virus (TGEV) is a porcine enteropathogenic coronavirus, causing acute swine enteric disease especially in suckling piglets. Mesoporous silica nanoparticles (MSNs) are safe vaccine adjuvant, which could enhance immune responses. Our previous research confirmed that nan...

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Autores principales:	Lanlan Zheng, Fujie Zhao, Jiaxi Ru, Lintao Liu, Zi Wang, Nianxiang Wang, Xiangli Shu, Zhanyong Wei, Huichen Guo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	inactivated TGEV vaccine nano silicon dendritic cell innate immunity Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e95511d80a104bd18240af296249c0f8
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spelling	oai:doaj.org-article:e95511d80a104bd18240af296249c0f82021-11-25T19:12:54ZEvaluation of the Effect of Inactivated Transmissible Gastroenteritis Virus Vaccine with Nano Silicon on the Phenotype and Function of Porcine Dendritic Cells10.3390/v131121581999-4915https://doaj.org/article/e95511d80a104bd18240af296249c0f82021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2158https://doaj.org/toc/1999-4915A transmissible gastroenteritis virus (TGEV) is a porcine enteropathogenic coronavirus, causing acute swine enteric disease especially in suckling piglets. Mesoporous silica nanoparticles (MSNs) are safe vaccine adjuvant, which could enhance immune responses. Our previous research confirmed that nano silicon had immune-enhancing effects with inactivated TGEV vaccine. In this study, we further clarified the immune-enhancing mechanism of the inactivated TGEV vaccine with MSNs on porcine dendritic cells (DCs). Our results indicated that the inactivated TGEV vaccine with MSNs strongly enhanced the activation of the DCs. Expressions of TLR3, TLR5, TLR7, TLR9, and TLR10, cytokines IFN-α, IL-1β, IL-6, IL-12, and TNF-α, cytokine receptor CCR-7 of immature DCs were characterized and showed themselves to be significantly higher in the inactivated TGEV vaccine with the MSN group. In summary, the inactivated TGEV vaccine with MSNs has effects on the phenotype and function of porcine DCs, which helps to better understand the immune-enhancing mechanism.Lanlan ZhengFujie ZhaoJiaxi RuLintao LiuZi WangNianxiang WangXiangli ShuZhanyong WeiHuichen GuoMDPI AGarticleinactivated TGEV vaccinenano silicondendritic cellinnate immunityMicrobiologyQR1-502ENViruses, Vol 13, Iss 2158, p 2158 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	inactivated TGEV vaccine nano silicon dendritic cell innate immunity Microbiology QR1-502
spellingShingle	inactivated TGEV vaccine nano silicon dendritic cell innate immunity Microbiology QR1-502 Lanlan Zheng Fujie Zhao Jiaxi Ru Lintao Liu Zi Wang Nianxiang Wang Xiangli Shu Zhanyong Wei Huichen Guo Evaluation of the Effect of Inactivated Transmissible Gastroenteritis Virus Vaccine with Nano Silicon on the Phenotype and Function of Porcine Dendritic Cells
description	A transmissible gastroenteritis virus (TGEV) is a porcine enteropathogenic coronavirus, causing acute swine enteric disease especially in suckling piglets. Mesoporous silica nanoparticles (MSNs) are safe vaccine adjuvant, which could enhance immune responses. Our previous research confirmed that nano silicon had immune-enhancing effects with inactivated TGEV vaccine. In this study, we further clarified the immune-enhancing mechanism of the inactivated TGEV vaccine with MSNs on porcine dendritic cells (DCs). Our results indicated that the inactivated TGEV vaccine with MSNs strongly enhanced the activation of the DCs. Expressions of TLR3, TLR5, TLR7, TLR9, and TLR10, cytokines IFN-α, IL-1β, IL-6, IL-12, and TNF-α, cytokine receptor CCR-7 of immature DCs were characterized and showed themselves to be significantly higher in the inactivated TGEV vaccine with the MSN group. In summary, the inactivated TGEV vaccine with MSNs has effects on the phenotype and function of porcine DCs, which helps to better understand the immune-enhancing mechanism.
format	article
author	Lanlan Zheng Fujie Zhao Jiaxi Ru Lintao Liu Zi Wang Nianxiang Wang Xiangli Shu Zhanyong Wei Huichen Guo
author_facet	Lanlan Zheng Fujie Zhao Jiaxi Ru Lintao Liu Zi Wang Nianxiang Wang Xiangli Shu Zhanyong Wei Huichen Guo
author_sort	Lanlan Zheng
title	Evaluation of the Effect of Inactivated Transmissible Gastroenteritis Virus Vaccine with Nano Silicon on the Phenotype and Function of Porcine Dendritic Cells
title_short	Evaluation of the Effect of Inactivated Transmissible Gastroenteritis Virus Vaccine with Nano Silicon on the Phenotype and Function of Porcine Dendritic Cells
title_full	Evaluation of the Effect of Inactivated Transmissible Gastroenteritis Virus Vaccine with Nano Silicon on the Phenotype and Function of Porcine Dendritic Cells
title_fullStr	Evaluation of the Effect of Inactivated Transmissible Gastroenteritis Virus Vaccine with Nano Silicon on the Phenotype and Function of Porcine Dendritic Cells
title_full_unstemmed	Evaluation of the Effect of Inactivated Transmissible Gastroenteritis Virus Vaccine with Nano Silicon on the Phenotype and Function of Porcine Dendritic Cells
title_sort	evaluation of the effect of inactivated transmissible gastroenteritis virus vaccine with nano silicon on the phenotype and function of porcine dendritic cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e95511d80a104bd18240af296249c0f8
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Evaluation of the Effect of Inactivated Transmissible Gastroenteritis Virus Vaccine with Nano Silicon on the Phenotype and Function of Porcine Dendritic Cells

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