Efficient B cell depletion via diphtheria toxin in CD19-Cre/iDTR mice.

B cells were first discovered as antibody producing cells, as B-1 B cells and finally as effector cells. In recent years their capacity to serve as antigen presenting cells is increasingly appreciated, and better tools are needed to study their function. We have previously described a new mouse mode...

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Autores principales:	Filiz Demircik, Thorsten Buch, Ari Waisman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e9556a14f0ba4301993a83e9e3161873
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spelling	oai:doaj.org-article:e9556a14f0ba4301993a83e9e31618732021-11-18T07:51:39ZEfficient B cell depletion via diphtheria toxin in CD19-Cre/iDTR mice.1932-620310.1371/journal.pone.0060643https://doaj.org/article/e9556a14f0ba4301993a83e9e31618732013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23544158/?tool=EBIhttps://doaj.org/toc/1932-6203B cells were first discovered as antibody producing cells, as B-1 B cells and finally as effector cells. In recent years their capacity to serve as antigen presenting cells is increasingly appreciated, and better tools are needed to study their function. We have previously described a new mouse model, the iDTR mice, that allow for the Cre-mediated expression of the diphtheria toxin receptor, thus rendering cells that express the Cre-recombinase sensitivity to diphtheria toxin. Herein we describe a new mouse line, the B-DTR mice, where the CD19-Cre was crossed to the iDTR mice. B-DTR allows for the efficient and cost-effective depletion of different B cell subpopulations, but only partially plasma cells. These mice can therefore be used to study the importance of B cells versus plasma cells in different immune responses and autoimmune diseases.Filiz DemircikThorsten BuchAri WaismanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e60643 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Filiz Demircik Thorsten Buch Ari Waisman Efficient B cell depletion via diphtheria toxin in CD19-Cre/iDTR mice.
description	B cells were first discovered as antibody producing cells, as B-1 B cells and finally as effector cells. In recent years their capacity to serve as antigen presenting cells is increasingly appreciated, and better tools are needed to study their function. We have previously described a new mouse model, the iDTR mice, that allow for the Cre-mediated expression of the diphtheria toxin receptor, thus rendering cells that express the Cre-recombinase sensitivity to diphtheria toxin. Herein we describe a new mouse line, the B-DTR mice, where the CD19-Cre was crossed to the iDTR mice. B-DTR allows for the efficient and cost-effective depletion of different B cell subpopulations, but only partially plasma cells. These mice can therefore be used to study the importance of B cells versus plasma cells in different immune responses and autoimmune diseases.
format	article
author	Filiz Demircik Thorsten Buch Ari Waisman
author_facet	Filiz Demircik Thorsten Buch Ari Waisman
author_sort	Filiz Demircik
title	Efficient B cell depletion via diphtheria toxin in CD19-Cre/iDTR mice.
title_short	Efficient B cell depletion via diphtheria toxin in CD19-Cre/iDTR mice.
title_full	Efficient B cell depletion via diphtheria toxin in CD19-Cre/iDTR mice.
title_fullStr	Efficient B cell depletion via diphtheria toxin in CD19-Cre/iDTR mice.
title_full_unstemmed	Efficient B cell depletion via diphtheria toxin in CD19-Cre/iDTR mice.
title_sort	efficient b cell depletion via diphtheria toxin in cd19-cre/idtr mice.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e9556a14f0ba4301993a83e9e3161873
work_keys_str_mv	AT filizdemircik efficientbcelldepletionviadiphtheriatoxinincd19creidtrmice AT thorstenbuch efficientbcelldepletionviadiphtheriatoxinincd19creidtrmice AT ariwaisman efficientbcelldepletionviadiphtheriatoxinincd19creidtrmice
_version_	1718422884223811584

Efficient B cell depletion via diphtheria toxin in CD19-Cre/iDTR mice.

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