Gold Nanoparticles-Loaded Polyvinylpyrrolidone/Ethylcellulose Coaxial Electrospun Nanofibers with Enhanced Osteogenic Capability for Bone Tissue Regeneration

Biomaterial-based scaffolds fabricated by electrospinning technique are promising platforms for bone tissue engineering. However, the current scaffolds have some limitations in terms of poor osteogenic bioactivities. In this study, citrate-stabilized gold-nanoparticles (GNPs) were encapsulated into...

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Autores principales:	Caoxing Huang, Jian Dong, Yunyang Zhang, Senlin Chai, Xucai Wang, Shixiong Kang, Dengguang Yu, Peng Wang, Qing Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Gold nanoparticles Coaxial electrospinning Osteogenic bioactivity Osteogenic differentiation Bone tissue regeneration Materials of engineering and construction. Mechanics of materials TA401-492
Acceso en línea:	https://doaj.org/article/e956c09c3b5d45068775935ba6945e8e
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spelling	oai:doaj.org-article:e956c09c3b5d45068775935ba6945e8e2021-11-28T04:27:41ZGold Nanoparticles-Loaded Polyvinylpyrrolidone/Ethylcellulose Coaxial Electrospun Nanofibers with Enhanced Osteogenic Capability for Bone Tissue Regeneration0264-127510.1016/j.matdes.2021.110240https://doaj.org/article/e956c09c3b5d45068775935ba6945e8e2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0264127521007954https://doaj.org/toc/0264-1275Biomaterial-based scaffolds fabricated by electrospinning technique are promising platforms for bone tissue engineering. However, the current scaffolds have some limitations in terms of poor osteogenic bioactivities. In this study, citrate-stabilized gold-nanoparticles (GNPs) were encapsulated into polyvinylpyrrolidone/ethylcellulose scaffolds fabricated by coaxial electrospinning technique. Three types of GNPs-loaded electrospun scaffolds (P/E-0.5, P/E-1, and P/E-1.5) were prepared by changing the feeding GNPs. The morphological and physicochemical properties of these GNPs-incorporated electrospun scaffolds were comprehensively characterized. The results demonstrated that GNPs were successfully encapsulated into electrospun scaffolds, and their addition barely affected the morphology but improved the porosity and mechanical properties. In vitro studies revealed that GNPs-incorporated electrospun scaffolds showed excellent biocompatibility and osteogenic bioactivities, wherein the alkaline phosphatase activity, mineralized nodule formation, and the osteogenic-related genes expression were enhanced in GNPs-incorporated electrospun scaffolds compared to the neat P/E electrospun nanofibers. Then, the GNPs-incorporated electrospun scaffolds were surgically implanted into the defect area of the rat skull bone to test their in vivo bone repairing effect. It was observed that GNPs-incorporated scaffolds rapidly accelerated bone regeneration in vivo. Taken together, GNPs-incorporated coaxial electrospun nanofibers might be considered as promising scaffolds in the field of bone tissue regeneration.Caoxing HuangJian DongYunyang ZhangSenlin ChaiXucai WangShixiong KangDengguang YuPeng WangQing JiangElsevierarticleGold nanoparticlesCoaxial electrospinningOsteogenic bioactivityOsteogenic differentiationBone tissue regenerationMaterials of engineering and construction. Mechanics of materialsTA401-492ENMaterials & Design, Vol 212, Iss , Pp 110240- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gold nanoparticles Coaxial electrospinning Osteogenic bioactivity Osteogenic differentiation Bone tissue regeneration Materials of engineering and construction. Mechanics of materials TA401-492
spellingShingle	Gold nanoparticles Coaxial electrospinning Osteogenic bioactivity Osteogenic differentiation Bone tissue regeneration Materials of engineering and construction. Mechanics of materials TA401-492 Caoxing Huang Jian Dong Yunyang Zhang Senlin Chai Xucai Wang Shixiong Kang Dengguang Yu Peng Wang Qing Jiang Gold Nanoparticles-Loaded Polyvinylpyrrolidone/Ethylcellulose Coaxial Electrospun Nanofibers with Enhanced Osteogenic Capability for Bone Tissue Regeneration
description	Biomaterial-based scaffolds fabricated by electrospinning technique are promising platforms for bone tissue engineering. However, the current scaffolds have some limitations in terms of poor osteogenic bioactivities. In this study, citrate-stabilized gold-nanoparticles (GNPs) were encapsulated into polyvinylpyrrolidone/ethylcellulose scaffolds fabricated by coaxial electrospinning technique. Three types of GNPs-loaded electrospun scaffolds (P/E-0.5, P/E-1, and P/E-1.5) were prepared by changing the feeding GNPs. The morphological and physicochemical properties of these GNPs-incorporated electrospun scaffolds were comprehensively characterized. The results demonstrated that GNPs were successfully encapsulated into electrospun scaffolds, and their addition barely affected the morphology but improved the porosity and mechanical properties. In vitro studies revealed that GNPs-incorporated electrospun scaffolds showed excellent biocompatibility and osteogenic bioactivities, wherein the alkaline phosphatase activity, mineralized nodule formation, and the osteogenic-related genes expression were enhanced in GNPs-incorporated electrospun scaffolds compared to the neat P/E electrospun nanofibers. Then, the GNPs-incorporated electrospun scaffolds were surgically implanted into the defect area of the rat skull bone to test their in vivo bone repairing effect. It was observed that GNPs-incorporated scaffolds rapidly accelerated bone regeneration in vivo. Taken together, GNPs-incorporated coaxial electrospun nanofibers might be considered as promising scaffolds in the field of bone tissue regeneration.
format	article
author	Caoxing Huang Jian Dong Yunyang Zhang Senlin Chai Xucai Wang Shixiong Kang Dengguang Yu Peng Wang Qing Jiang
author_facet	Caoxing Huang Jian Dong Yunyang Zhang Senlin Chai Xucai Wang Shixiong Kang Dengguang Yu Peng Wang Qing Jiang
author_sort	Caoxing Huang
title	Gold Nanoparticles-Loaded Polyvinylpyrrolidone/Ethylcellulose Coaxial Electrospun Nanofibers with Enhanced Osteogenic Capability for Bone Tissue Regeneration
title_short	Gold Nanoparticles-Loaded Polyvinylpyrrolidone/Ethylcellulose Coaxial Electrospun Nanofibers with Enhanced Osteogenic Capability for Bone Tissue Regeneration
title_full	Gold Nanoparticles-Loaded Polyvinylpyrrolidone/Ethylcellulose Coaxial Electrospun Nanofibers with Enhanced Osteogenic Capability for Bone Tissue Regeneration
title_fullStr	Gold Nanoparticles-Loaded Polyvinylpyrrolidone/Ethylcellulose Coaxial Electrospun Nanofibers with Enhanced Osteogenic Capability for Bone Tissue Regeneration
title_full_unstemmed	Gold Nanoparticles-Loaded Polyvinylpyrrolidone/Ethylcellulose Coaxial Electrospun Nanofibers with Enhanced Osteogenic Capability for Bone Tissue Regeneration
title_sort	gold nanoparticles-loaded polyvinylpyrrolidone/ethylcellulose coaxial electrospun nanofibers with enhanced osteogenic capability for bone tissue regeneration
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/e956c09c3b5d45068775935ba6945e8e
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_version_	1718408413645373440

Gold Nanoparticles-Loaded Polyvinylpyrrolidone/Ethylcellulose Coaxial Electrospun Nanofibers with Enhanced Osteogenic Capability for Bone Tissue Regeneration

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