Mycorrhiza-Induced Alterations in Metabolome of <i>Medicago lupulina</i> Leaves during Symbiosis Development
The present study is aimed at disclosing metabolic profile alterations in the leaves of the <i>Medicago lupulina</i> MlS-1 line that result from high-efficiency arbuscular mycorrhiza (AM) symbiosis formed with <i>Rhizophagus irregularis</i> under condition of a low phosphorus...
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| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | article |
| Language: | EN |
| Published: |
MDPI AG
2021
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| Subjects: | |
| Online Access: | https://doaj.org/article/e95f812e78c74937a6cfd3ea3b850a4d |
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| Summary: | The present study is aimed at disclosing metabolic profile alterations in the leaves of the <i>Medicago lupulina</i> MlS-1 line that result from high-efficiency arbuscular mycorrhiza (AM) symbiosis formed with <i>Rhizophagus irregularis</i> under condition of a low phosphorus level in the substrate. A highly effective AM symbiosis was established in the period from the stooling to the shoot branching initiation stage (the efficiency in stem height exceeded 200%). Mycorrhization led to a more intensive accumulation of phosphates (glycerophosphoglycerol and inorganic phosphate) in <i>M. lupulina</i> leaves. Metabolic spectra were detected with GS-MS analysis. The application of complex mathematical analyses made it possible to identify the clustering of various groups of 320 metabolites and thus demonstrate the central importance of the carbohydrate and carboxylate-amino acid clusters. The results obtained indicate a delay in the metabolic development of mycorrhized plants. Thus, AM not only accelerates the transition between plant developmental stages but delays biochemical “maturation” mainly in the form of a lag of sugar accumulation in comparison with non-mycorrhized plants. Several methods of statistical modeling proved that, at least with respect to determining the metabolic status of host-plant leaves, stages of phenological development have priority over calendar age. |
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