Mycorrhiza-Induced Alterations in Metabolome of Medicago lupulina Leaves during Symbiosis Development

The present study is aimed at disclosing metabolic profile alterations in the leaves of the Medicago lupulina MlS-1 line that result from high-efficiency arbuscular mycorrhiza (AM) symbiosis formed with Rhizophagus irregularis under condition of a low phosphorus...

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Autores principales:	Andrey P. Yurkov, Roman K. Puzanskiy, Galina S. Avdeeva, Lidija M. Jacobi, Anastasia O. Gorbunova, Alexey A. Kryukov, Andrei P. Kozhemyakov, Yuri V. Laktionov, Yuri V. Kosulnikov, Daria A. Romanyuk, Vladislav V. Yemelyanov, Alexey L. Shavarda, Anastasia A. Kirpichnikova, Galina N. Smolikova, Maria F. Shishova
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Medicago lupulina</i> arbuscular mycorrhiza <i>Rhizophagus irregularis</i> symbiotic efficiency plant development physiological stage Botany QK1-989
Acceso en línea:	https://doaj.org/article/e95f812e78c74937a6cfd3ea3b850a4d
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spelling	oai:doaj.org-article:e95f812e78c74937a6cfd3ea3b850a4d2021-11-25T18:47:09ZMycorrhiza-Induced Alterations in Metabolome of <i>Medicago lupulina</i> Leaves during Symbiosis Development10.3390/plants101125062223-7747https://doaj.org/article/e95f812e78c74937a6cfd3ea3b850a4d2021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2506https://doaj.org/toc/2223-7747The present study is aimed at disclosing metabolic profile alterations in the leaves of the <i>Medicago lupulina</i> MlS-1 line that result from high-efficiency arbuscular mycorrhiza (AM) symbiosis formed with <i>Rhizophagus irregularis</i> under condition of a low phosphorus level in the substrate. A highly effective AM symbiosis was established in the period from the stooling to the shoot branching initiation stage (the efficiency in stem height exceeded 200%). Mycorrhization led to a more intensive accumulation of phosphates (glycerophosphoglycerol and inorganic phosphate) in <i>M. lupulina</i> leaves. Metabolic spectra were detected with GS-MS analysis. The application of complex mathematical analyses made it possible to identify the clustering of various groups of 320 metabolites and thus demonstrate the central importance of the carbohydrate and carboxylate-amino acid clusters. The results obtained indicate a delay in the metabolic development of mycorrhized plants. Thus, AM not only accelerates the transition between plant developmental stages but delays biochemical “maturation” mainly in the form of a lag of sugar accumulation in comparison with non-mycorrhized plants. Several methods of statistical modeling proved that, at least with respect to determining the metabolic status of host-plant leaves, stages of phenological development have priority over calendar age.Andrey P. YurkovRoman K. PuzanskiyGalina S. AvdeevaLidija M. JacobiAnastasia O. GorbunovaAlexey A. KryukovAndrei P. KozhemyakovYuri V. LaktionovYuri V. KosulnikovDaria A. RomanyukVladislav V. YemelyanovAlexey L. ShavardaAnastasia A. KirpichnikovaGalina N. SmolikovaMaria F. ShishovaMDPI AGarticle<i>Medicago lupulina</i>arbuscular mycorrhiza<i>Rhizophagus irregularis</i>symbiotic efficiencyplant developmentphysiological stageBotanyQK1-989ENPlants, Vol 10, Iss 2506, p 2506 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Medicago lupulina</i> arbuscular mycorrhiza <i>Rhizophagus irregularis</i> symbiotic efficiency plant development physiological stage Botany QK1-989
spellingShingle	<i>Medicago lupulina</i> arbuscular mycorrhiza <i>Rhizophagus irregularis</i> symbiotic efficiency plant development physiological stage Botany QK1-989 Andrey P. Yurkov Roman K. Puzanskiy Galina S. Avdeeva Lidija M. Jacobi Anastasia O. Gorbunova Alexey A. Kryukov Andrei P. Kozhemyakov Yuri V. Laktionov Yuri V. Kosulnikov Daria A. Romanyuk Vladislav V. Yemelyanov Alexey L. Shavarda Anastasia A. Kirpichnikova Galina N. Smolikova Maria F. Shishova Mycorrhiza-Induced Alterations in Metabolome of <i>Medicago lupulina</i> Leaves during Symbiosis Development
description	The present study is aimed at disclosing metabolic profile alterations in the leaves of the <i>Medicago lupulina</i> MlS-1 line that result from high-efficiency arbuscular mycorrhiza (AM) symbiosis formed with <i>Rhizophagus irregularis</i> under condition of a low phosphorus level in the substrate. A highly effective AM symbiosis was established in the period from the stooling to the shoot branching initiation stage (the efficiency in stem height exceeded 200%). Mycorrhization led to a more intensive accumulation of phosphates (glycerophosphoglycerol and inorganic phosphate) in <i>M. lupulina</i> leaves. Metabolic spectra were detected with GS-MS analysis. The application of complex mathematical analyses made it possible to identify the clustering of various groups of 320 metabolites and thus demonstrate the central importance of the carbohydrate and carboxylate-amino acid clusters. The results obtained indicate a delay in the metabolic development of mycorrhized plants. Thus, AM not only accelerates the transition between plant developmental stages but delays biochemical “maturation” mainly in the form of a lag of sugar accumulation in comparison with non-mycorrhized plants. Several methods of statistical modeling proved that, at least with respect to determining the metabolic status of host-plant leaves, stages of phenological development have priority over calendar age.
format	article
author	Andrey P. Yurkov Roman K. Puzanskiy Galina S. Avdeeva Lidija M. Jacobi Anastasia O. Gorbunova Alexey A. Kryukov Andrei P. Kozhemyakov Yuri V. Laktionov Yuri V. Kosulnikov Daria A. Romanyuk Vladislav V. Yemelyanov Alexey L. Shavarda Anastasia A. Kirpichnikova Galina N. Smolikova Maria F. Shishova
author_facet	Andrey P. Yurkov Roman K. Puzanskiy Galina S. Avdeeva Lidija M. Jacobi Anastasia O. Gorbunova Alexey A. Kryukov Andrei P. Kozhemyakov Yuri V. Laktionov Yuri V. Kosulnikov Daria A. Romanyuk Vladislav V. Yemelyanov Alexey L. Shavarda Anastasia A. Kirpichnikova Galina N. Smolikova Maria F. Shishova
author_sort	Andrey P. Yurkov
title	Mycorrhiza-Induced Alterations in Metabolome of <i>Medicago lupulina</i> Leaves during Symbiosis Development
title_short	Mycorrhiza-Induced Alterations in Metabolome of <i>Medicago lupulina</i> Leaves during Symbiosis Development
title_full	Mycorrhiza-Induced Alterations in Metabolome of <i>Medicago lupulina</i> Leaves during Symbiosis Development
title_fullStr	Mycorrhiza-Induced Alterations in Metabolome of <i>Medicago lupulina</i> Leaves during Symbiosis Development
title_full_unstemmed	Mycorrhiza-Induced Alterations in Metabolome of <i>Medicago lupulina</i> Leaves during Symbiosis Development
title_sort	mycorrhiza-induced alterations in metabolome of <i>medicago lupulina</i> leaves during symbiosis development
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e95f812e78c74937a6cfd3ea3b850a4d
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Mycorrhiza-Induced Alterations in Metabolome of <i>Medicago lupulina</i> Leaves during Symbiosis Development

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