Single-Cell RNA Sequencing Reveals Heterogeneity and Functional Diversity of Lymphatic Endothelial Cells

Lymphatic endothelial cells (LECs) line the lymphatic vasculature and play a central role in the immune response. LECs have abilities to regulate immune transport, to promote immune cell survival, and to cross present antigens to dendritic cells. Single-cell RNA sequencing (scRNA) technology has acc...

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Autores principales:	Hannah den Braanker, Astrid C. van Stigt, Marc R. Kok, Erik Lubberts, Radjesh J. Bisoendial
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	lymphatics lymphatic endothelial cells single cell RNA-sequencing lymph nodes inflammation Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/e95fc12fb7164a1dad51096139348b36
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spelling	oai:doaj.org-article:e95fc12fb7164a1dad51096139348b362021-11-11T17:23:55ZSingle-Cell RNA Sequencing Reveals Heterogeneity and Functional Diversity of Lymphatic Endothelial Cells10.3390/ijms2221119761422-00671661-6596https://doaj.org/article/e95fc12fb7164a1dad51096139348b362021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11976https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Lymphatic endothelial cells (LECs) line the lymphatic vasculature and play a central role in the immune response. LECs have abilities to regulate immune transport, to promote immune cell survival, and to cross present antigens to dendritic cells. Single-cell RNA sequencing (scRNA) technology has accelerated new discoveries in the field of lymphatic vascular biology. This review will summarize these new findings in regard to embryonic development, LEC heterogeneity with associated functional diversity, and interactions with other cells. Depending on the organ, location in the lymphatic vascular tree, and micro-environmental conditions, LECs feature unique properties and tasks. Furthermore, adjacent stromal cells need the support of LECs for fulfilling their tasks in the immune response, such as immune cell transport and antigen presentation. Although aberrant lymphatic vasculature has been observed in a number of chronic inflammatory diseases, the knowledge on LEC heterogeneity and functional diversity in these diseases is limited. Combining scRNA sequencing data with imaging and more in-depth functional experiments will advance our knowledge of LECs in health and disease. Building the case, the LEC could be put forward as a new therapeutic target in chronic inflammatory diseases, counterweighting the current immune-cell focused therapies.Hannah den BraankerAstrid C. van StigtMarc R. KokErik LubbertsRadjesh J. BisoendialMDPI AGarticlelymphaticslymphatic endothelial cellssingle cell RNA-sequencinglymph nodesinflammationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11976, p 11976 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lymphatics lymphatic endothelial cells single cell RNA-sequencing lymph nodes inflammation Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	lymphatics lymphatic endothelial cells single cell RNA-sequencing lymph nodes inflammation Biology (General) QH301-705.5 Chemistry QD1-999 Hannah den Braanker Astrid C. van Stigt Marc R. Kok Erik Lubberts Radjesh J. Bisoendial Single-Cell RNA Sequencing Reveals Heterogeneity and Functional Diversity of Lymphatic Endothelial Cells
description	Lymphatic endothelial cells (LECs) line the lymphatic vasculature and play a central role in the immune response. LECs have abilities to regulate immune transport, to promote immune cell survival, and to cross present antigens to dendritic cells. Single-cell RNA sequencing (scRNA) technology has accelerated new discoveries in the field of lymphatic vascular biology. This review will summarize these new findings in regard to embryonic development, LEC heterogeneity with associated functional diversity, and interactions with other cells. Depending on the organ, location in the lymphatic vascular tree, and micro-environmental conditions, LECs feature unique properties and tasks. Furthermore, adjacent stromal cells need the support of LECs for fulfilling their tasks in the immune response, such as immune cell transport and antigen presentation. Although aberrant lymphatic vasculature has been observed in a number of chronic inflammatory diseases, the knowledge on LEC heterogeneity and functional diversity in these diseases is limited. Combining scRNA sequencing data with imaging and more in-depth functional experiments will advance our knowledge of LECs in health and disease. Building the case, the LEC could be put forward as a new therapeutic target in chronic inflammatory diseases, counterweighting the current immune-cell focused therapies.
format	article
author	Hannah den Braanker Astrid C. van Stigt Marc R. Kok Erik Lubberts Radjesh J. Bisoendial
author_facet	Hannah den Braanker Astrid C. van Stigt Marc R. Kok Erik Lubberts Radjesh J. Bisoendial
author_sort	Hannah den Braanker
title	Single-Cell RNA Sequencing Reveals Heterogeneity and Functional Diversity of Lymphatic Endothelial Cells
title_short	Single-Cell RNA Sequencing Reveals Heterogeneity and Functional Diversity of Lymphatic Endothelial Cells
title_full	Single-Cell RNA Sequencing Reveals Heterogeneity and Functional Diversity of Lymphatic Endothelial Cells
title_fullStr	Single-Cell RNA Sequencing Reveals Heterogeneity and Functional Diversity of Lymphatic Endothelial Cells
title_full_unstemmed	Single-Cell RNA Sequencing Reveals Heterogeneity and Functional Diversity of Lymphatic Endothelial Cells
title_sort	single-cell rna sequencing reveals heterogeneity and functional diversity of lymphatic endothelial cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e95fc12fb7164a1dad51096139348b36
work_keys_str_mv	AT hannahdenbraanker singlecellrnasequencingrevealsheterogeneityandfunctionaldiversityoflymphaticendothelialcells AT astridcvanstigt singlecellrnasequencingrevealsheterogeneityandfunctionaldiversityoflymphaticendothelialcells AT marcrkok singlecellrnasequencingrevealsheterogeneityandfunctionaldiversityoflymphaticendothelialcells AT eriklubberts singlecellrnasequencingrevealsheterogeneityandfunctionaldiversityoflymphaticendothelialcells AT radjeshjbisoendial singlecellrnasequencingrevealsheterogeneityandfunctionaldiversityoflymphaticendothelialcells
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Single-Cell RNA Sequencing Reveals Heterogeneity and Functional Diversity of Lymphatic Endothelial Cells

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