Quantification of Phytophthora infestans population densities and their changes in potato field soil using real-time PCR

Abstract Tuber infection of Phytophthora infestans often occurs at harvest. However, it is difficult to accurately estimate the population densities of P. infestans in soil, especially Japanese soil. In the present study, P. infestans DNA was extracted from soil samples using a modified CTAB-bead me...

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Autores principales:	Hisashi Osawa, Nobuyuki Suzuki, Seishi Akino, Hiromichi Araki, Kenji Asano, Kotaro Akai, Norio Kondo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e963c0e775744a2a907a799a869391d9
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spelling	oai:doaj.org-article:e963c0e775744a2a907a799a869391d92021-12-02T13:18:08ZQuantification of Phytophthora infestans population densities and their changes in potato field soil using real-time PCR10.1038/s41598-021-85492-z2045-2322https://doaj.org/article/e963c0e775744a2a907a799a869391d92021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85492-zhttps://doaj.org/toc/2045-2322Abstract Tuber infection of Phytophthora infestans often occurs at harvest. However, it is difficult to accurately estimate the population densities of P. infestans in soil, especially Japanese soil. In the present study, P. infestans DNA was extracted from soil samples using a modified CTAB-bead method and quantified using real-time PCR to accurately, rapidly and easily estimate the P. infestans population densities in upland soils in Japan. P. infestans was well quantified in eleven types of soil samples, including nine types of upland soils in Japan, that were artificially inoculated with a zoosporangia suspension. The amounts of P. infestans DNA estimated by the real-time PCR were proportional to the inoculum densities. In the non-controlled experimental potato field, P. infestans population densities in soil corresponded to the development of symptoms and were correlated with the number of lesions on the potato foliage. These results imply that the proposed real-time PCR assay is suitable for the estimation or monitoring of P. infestans population densities in upland soils in Japan. The population densities at the ridge bottoms were larger than those at any other location in commercial potato fields. These results were similar to those of a previous report using a bioassay. Moreover, a correlation between DNA quantity and inoculum potential was observed. In conclusion, the real-time PCR assay developed in this study is suitable for indirect estimation of the inoculum potential of P. infestans.Hisashi OsawaNobuyuki SuzukiSeishi AkinoHiromichi ArakiKenji AsanoKotaro AkaiNorio KondoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hisashi Osawa Nobuyuki Suzuki Seishi Akino Hiromichi Araki Kenji Asano Kotaro Akai Norio Kondo Quantification of Phytophthora infestans population densities and their changes in potato field soil using real-time PCR
description	Abstract Tuber infection of Phytophthora infestans often occurs at harvest. However, it is difficult to accurately estimate the population densities of P. infestans in soil, especially Japanese soil. In the present study, P. infestans DNA was extracted from soil samples using a modified CTAB-bead method and quantified using real-time PCR to accurately, rapidly and easily estimate the P. infestans population densities in upland soils in Japan. P. infestans was well quantified in eleven types of soil samples, including nine types of upland soils in Japan, that were artificially inoculated with a zoosporangia suspension. The amounts of P. infestans DNA estimated by the real-time PCR were proportional to the inoculum densities. In the non-controlled experimental potato field, P. infestans population densities in soil corresponded to the development of symptoms and were correlated with the number of lesions on the potato foliage. These results imply that the proposed real-time PCR assay is suitable for the estimation or monitoring of P. infestans population densities in upland soils in Japan. The population densities at the ridge bottoms were larger than those at any other location in commercial potato fields. These results were similar to those of a previous report using a bioassay. Moreover, a correlation between DNA quantity and inoculum potential was observed. In conclusion, the real-time PCR assay developed in this study is suitable for indirect estimation of the inoculum potential of P. infestans.
format	article
author	Hisashi Osawa Nobuyuki Suzuki Seishi Akino Hiromichi Araki Kenji Asano Kotaro Akai Norio Kondo
author_facet	Hisashi Osawa Nobuyuki Suzuki Seishi Akino Hiromichi Araki Kenji Asano Kotaro Akai Norio Kondo
author_sort	Hisashi Osawa
title	Quantification of Phytophthora infestans population densities and their changes in potato field soil using real-time PCR
title_short	Quantification of Phytophthora infestans population densities and their changes in potato field soil using real-time PCR
title_full	Quantification of Phytophthora infestans population densities and their changes in potato field soil using real-time PCR
title_fullStr	Quantification of Phytophthora infestans population densities and their changes in potato field soil using real-time PCR
title_full_unstemmed	Quantification of Phytophthora infestans population densities and their changes in potato field soil using real-time PCR
title_sort	quantification of phytophthora infestans population densities and their changes in potato field soil using real-time pcr
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e963c0e775744a2a907a799a869391d9
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Quantification of Phytophthora infestans population densities and their changes in potato field soil using real-time PCR

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