Granulocyte colony stimulating factor induces lipopolysaccharide (LPS) sensitization via upregulation of LPS binding protein in rat.

Liver is the main organ for lipopolysaccharide (LPS) clearance. Sensitization to LPS is associated with the upregulation of LPS-binding protein (LBP) in animal models. Therefore, we hypothesized that LBP could induce LPS sensitization through enhancing hepatic uptake of LPS. In this study, we examin...

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Auteurs principaux:	Haoshu Fang, Anding Liu, Jian Sun, Alexandra Kitz, Olaf Dirsch, Uta Dahmen
Format:	article
Langue:	EN
Publié:	Public Library of Science (PLoS) 2013
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/e964605d06d947e4b791ee0327874ae1
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spelling	oai:doaj.org-article:e964605d06d947e4b791ee0327874ae12021-11-18T07:56:50ZGranulocyte colony stimulating factor induces lipopolysaccharide (LPS) sensitization via upregulation of LPS binding protein in rat.1932-620310.1371/journal.pone.0056654https://doaj.org/article/e964605d06d947e4b791ee0327874ae12013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23437199/?tool=EBIhttps://doaj.org/toc/1932-6203Liver is the main organ for lipopolysaccharide (LPS) clearance. Sensitization to LPS is associated with the upregulation of LPS-binding protein (LBP) in animal models. Therefore, we hypothesized that LBP could induce LPS sensitization through enhancing hepatic uptake of LPS. In this study, we examined the role of LBP in pathogenesis of LPS induced systemic inflammatory response syndrome (SIRS). LBP expression was upregulated after granulocyte colony stimulating (G-CSF) pretreatment. The effect of LBP was further confirmed by blockade of LBP using LBP blocking peptide--LBPK95A. After G-CSF pretreatment, upregulation of LBP was observed in bone marrow cells and liver. The G-CSF induced LBP upregulation caused LPS hypersensitization in rats as indicated by higher mortality and severer liver damage. Of note, LBP blockade increased the survival rate and attenuated the liver injury. The LBP induced LPS hypersensitization was associated with increased hepatic uptake of LPS and augmented hepatic expression of LPS receptors, such as toll-like receptor (TLR)-4. Furthermore, LBP mediated early neutrophil infiltration, which led to increased monocyte recruitment in liver after LPS administration. In conclusion, G-CSF induced LBP expression could serve as a new model for investigation of LPS sensitization. We demonstrated the crucial role of LBP upregulation in pathogenesis of LPS induced SIRS.Haoshu FangAnding LiuJian SunAlexandra KitzOlaf DirschUta DahmenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e56654 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Haoshu Fang Anding Liu Jian Sun Alexandra Kitz Olaf Dirsch Uta Dahmen Granulocyte colony stimulating factor induces lipopolysaccharide (LPS) sensitization via upregulation of LPS binding protein in rat.
description	Liver is the main organ for lipopolysaccharide (LPS) clearance. Sensitization to LPS is associated with the upregulation of LPS-binding protein (LBP) in animal models. Therefore, we hypothesized that LBP could induce LPS sensitization through enhancing hepatic uptake of LPS. In this study, we examined the role of LBP in pathogenesis of LPS induced systemic inflammatory response syndrome (SIRS). LBP expression was upregulated after granulocyte colony stimulating (G-CSF) pretreatment. The effect of LBP was further confirmed by blockade of LBP using LBP blocking peptide--LBPK95A. After G-CSF pretreatment, upregulation of LBP was observed in bone marrow cells and liver. The G-CSF induced LBP upregulation caused LPS hypersensitization in rats as indicated by higher mortality and severer liver damage. Of note, LBP blockade increased the survival rate and attenuated the liver injury. The LBP induced LPS hypersensitization was associated with increased hepatic uptake of LPS and augmented hepatic expression of LPS receptors, such as toll-like receptor (TLR)-4. Furthermore, LBP mediated early neutrophil infiltration, which led to increased monocyte recruitment in liver after LPS administration. In conclusion, G-CSF induced LBP expression could serve as a new model for investigation of LPS sensitization. We demonstrated the crucial role of LBP upregulation in pathogenesis of LPS induced SIRS.
format	article
author	Haoshu Fang Anding Liu Jian Sun Alexandra Kitz Olaf Dirsch Uta Dahmen
author_facet	Haoshu Fang Anding Liu Jian Sun Alexandra Kitz Olaf Dirsch Uta Dahmen
author_sort	Haoshu Fang
title	Granulocyte colony stimulating factor induces lipopolysaccharide (LPS) sensitization via upregulation of LPS binding protein in rat.
title_short	Granulocyte colony stimulating factor induces lipopolysaccharide (LPS) sensitization via upregulation of LPS binding protein in rat.
title_full	Granulocyte colony stimulating factor induces lipopolysaccharide (LPS) sensitization via upregulation of LPS binding protein in rat.
title_fullStr	Granulocyte colony stimulating factor induces lipopolysaccharide (LPS) sensitization via upregulation of LPS binding protein in rat.
title_full_unstemmed	Granulocyte colony stimulating factor induces lipopolysaccharide (LPS) sensitization via upregulation of LPS binding protein in rat.
title_sort	granulocyte colony stimulating factor induces lipopolysaccharide (lps) sensitization via upregulation of lps binding protein in rat.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e964605d06d947e4b791ee0327874ae1
work_keys_str_mv	AT haoshufang granulocytecolonystimulatingfactorinduceslipopolysaccharidelpssensitizationviaupregulationoflpsbindingproteininrat AT andingliu granulocytecolonystimulatingfactorinduceslipopolysaccharidelpssensitizationviaupregulationoflpsbindingproteininrat AT jiansun granulocytecolonystimulatingfactorinduceslipopolysaccharidelpssensitizationviaupregulationoflpsbindingproteininrat AT alexandrakitz granulocytecolonystimulatingfactorinduceslipopolysaccharidelpssensitizationviaupregulationoflpsbindingproteininrat AT olafdirsch granulocytecolonystimulatingfactorinduceslipopolysaccharidelpssensitizationviaupregulationoflpsbindingproteininrat AT utadahmen granulocytecolonystimulatingfactorinduceslipopolysaccharidelpssensitizationviaupregulationoflpsbindingproteininrat
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Granulocyte colony stimulating factor induces lipopolysaccharide (LPS) sensitization via upregulation of LPS binding protein in rat.

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