<italic toggle="yes">EFG1</italic> Mutations, Phenotypic Switching, and Colonization by Clinical a/α Strains of <named-content content-type="genus-species">Candida albicans</named-content>

ABSTRACT The transcription factor EFG1 functions as a suppressor of white-to-opaque and white-to-gray switching in a/α strains of Candida albicans. In a collection of 27 clinical isolates, 4 of the 17 EFG1/EFG1 strains, 1 of the 2 EFG1/efg1 strains, and all 8 of the efg1/efg1 strains underwent white...

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Autores principales:	Yang-Nim Park, Kayla Conway, Claude Pujol, Karla J. Daniels, David R. Soll
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Candida albicans EFG1 mutation clinical isolates switching to opaque Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e964f6a2b1a540c49c29e154fde26382
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spelling	oai:doaj.org-article:e964f6a2b1a540c49c29e154fde263822021-11-15T15:27:53Z<italic toggle="yes">EFG1</italic> Mutations, Phenotypic Switching, and Colonization by Clinical a/α Strains of <named-content content-type="genus-species">Candida albicans</named-content>10.1128/mSphere.00795-192379-5042https://doaj.org/article/e964f6a2b1a540c49c29e154fde263822020-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00795-19https://doaj.org/toc/2379-5042ABSTRACT The transcription factor EFG1 functions as a suppressor of white-to-opaque and white-to-gray switching in a/α strains of Candida albicans. In a collection of 27 clinical isolates, 4 of the 17 EFG1/EFG1 strains, 1 of the 2 EFG1/efg1 strains, and all 8 of the efg1/efg1 strains underwent white-to-opaque switching. The four EFG1/EFG1 strains, the one EFG1/efg1 strain, and one of the eight efg1/efg1 strains that underwent switching to opaque did not switch to gray and could not be complemented with a copy of EFG1. Competition experiments in a mouse model for gastrointestinal (GI) colonization confirmed that efg1/efg1 cells rapidly outcompete EFG1/EFG1 cells, and in plating experiments, formed colonies containing both gray and opaque cells. Direct microscopic analysis of live cells in the feces, however, revealed that the great majority of cells were opaque, suggesting opaque, not gray, may be the dominant phenotype at the site of colonization. IMPORTANCE Close to half of a collection of 27 clinical a/α isolates of Candida albicans underwent white-to-opaque switching. Complementation experiments revealed that while approximately half of the a/α switchers were due to EFG1 mutations, the remaining half were due to mutations in other genes. In addition, the results of competition experiments in a mouse GI tract colonization model support previous observations that efg1/efg1 cells rapidly outcompete EFG1/EFG1 strains, but direct microscopic analysis reveals that the major colonizing cells were opaque, not gray.Yang-Nim ParkKayla ConwayClaude PujolKarla J. DanielsDavid R. SollAmerican Society for MicrobiologyarticleCandida albicansEFG1 mutationclinical isolatesswitching to opaqueMicrobiologyQR1-502ENmSphere, Vol 5, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Candida albicans EFG1 mutation clinical isolates switching to opaque Microbiology QR1-502
spellingShingle	Candida albicans EFG1 mutation clinical isolates switching to opaque Microbiology QR1-502 Yang-Nim Park Kayla Conway Claude Pujol Karla J. Daniels David R. Soll <italic toggle="yes">EFG1</italic> Mutations, Phenotypic Switching, and Colonization by Clinical a/α Strains of <named-content content-type="genus-species">Candida albicans</named-content>
description	ABSTRACT The transcription factor EFG1 functions as a suppressor of white-to-opaque and white-to-gray switching in a/α strains of Candida albicans. In a collection of 27 clinical isolates, 4 of the 17 EFG1/EFG1 strains, 1 of the 2 EFG1/efg1 strains, and all 8 of the efg1/efg1 strains underwent white-to-opaque switching. The four EFG1/EFG1 strains, the one EFG1/efg1 strain, and one of the eight efg1/efg1 strains that underwent switching to opaque did not switch to gray and could not be complemented with a copy of EFG1. Competition experiments in a mouse model for gastrointestinal (GI) colonization confirmed that efg1/efg1 cells rapidly outcompete EFG1/EFG1 cells, and in plating experiments, formed colonies containing both gray and opaque cells. Direct microscopic analysis of live cells in the feces, however, revealed that the great majority of cells were opaque, suggesting opaque, not gray, may be the dominant phenotype at the site of colonization. IMPORTANCE Close to half of a collection of 27 clinical a/α isolates of Candida albicans underwent white-to-opaque switching. Complementation experiments revealed that while approximately half of the a/α switchers were due to EFG1 mutations, the remaining half were due to mutations in other genes. In addition, the results of competition experiments in a mouse GI tract colonization model support previous observations that efg1/efg1 cells rapidly outcompete EFG1/EFG1 strains, but direct microscopic analysis reveals that the major colonizing cells were opaque, not gray.
format	article
author	Yang-Nim Park Kayla Conway Claude Pujol Karla J. Daniels David R. Soll
author_facet	Yang-Nim Park Kayla Conway Claude Pujol Karla J. Daniels David R. Soll
author_sort	Yang-Nim Park
title	<italic toggle="yes">EFG1</italic> Mutations, Phenotypic Switching, and Colonization by Clinical a/α Strains of <named-content content-type="genus-species">Candida albicans</named-content>
title_short	<italic toggle="yes">EFG1</italic> Mutations, Phenotypic Switching, and Colonization by Clinical a/α Strains of <named-content content-type="genus-species">Candida albicans</named-content>
title_full	<italic toggle="yes">EFG1</italic> Mutations, Phenotypic Switching, and Colonization by Clinical a/α Strains of <named-content content-type="genus-species">Candida albicans</named-content>
title_fullStr	<italic toggle="yes">EFG1</italic> Mutations, Phenotypic Switching, and Colonization by Clinical a/α Strains of <named-content content-type="genus-species">Candida albicans</named-content>
title_full_unstemmed	<italic toggle="yes">EFG1</italic> Mutations, Phenotypic Switching, and Colonization by Clinical a/α Strains of <named-content content-type="genus-species">Candida albicans</named-content>
title_sort	<italic toggle="yes">efg1</italic> mutations, phenotypic switching, and colonization by clinical a/α strains of <named-content content-type="genus-species">candida albicans</named-content>
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/e964f6a2b1a540c49c29e154fde26382
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<italic toggle="yes">EFG1</italic> Mutations, Phenotypic Switching, and Colonization by Clinical a/α Strains of <named-content content-type="genus-species">Candida albicans</named-content>

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