Rapid detection of Mycoplasma mycoides subsp. capri and Mycoplasma capricolum subsp. capripneumoniae using high-resolution melting curve analysis

Abstract Mycoplasma capricolum subsp.subsp. capripneumonia (Mccp) and Mycoplasma mycoides subsp.sbusp. capri (Mmc) cause caprine pleuropneumonia (CCPP) and mycoplasmal pneumonia in goats and sheep (MPGS), respectively. These diseases cannot be identified on clinical symptoms alone and it is laboriou...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jing-peng Zhang, Zhi-cheng Liu, Jin-xiu Jiang, Yu-sheng Lin, Wei You, Qi-lin Hu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e968a1046fee4b089d493df13191c421
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e968a1046fee4b089d493df13191c421
record_format	dspace
spelling	oai:doaj.org-article:e968a1046fee4b089d493df13191c4212021-12-02T16:24:59ZRapid detection of Mycoplasma mycoides subsp. capri and Mycoplasma capricolum subsp. capripneumoniae using high-resolution melting curve analysis10.1038/s41598-021-93981-42045-2322https://doaj.org/article/e968a1046fee4b089d493df13191c4212021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93981-4https://doaj.org/toc/2045-2322Abstract Mycoplasma capricolum subsp.subsp. capripneumonia (Mccp) and Mycoplasma mycoides subsp.sbusp. capri (Mmc) cause caprine pleuropneumonia (CCPP) and mycoplasmal pneumonia in goats and sheep (MPGS), respectively. These diseases cannot be identified on clinical symptoms alone and it is laborious to distinguish them using biochemical methods. It is therefore important to establish a simple, rapid identification method for Mccp and Mmc. Here, we report a high-resolution melting (HRM) curve analysis using specific primers based on the Mmc 95010 strain MLC_0560 and Mccp F38 strain MCCPF38_00984 gene sequences. The method was highly specific with intra- and inter-batch coefficients of variation < 1%. The lower limit of detection for Mccp and Mmc was 55 copies/μL and 58 copies/μL, respectively. HRM and fluorescence qPCR results were compared using 106 nasal swabs and 47 lung tissue samples from goats (HRM-qPCR coincidence rate 94.8%; 145/153). Mycoplasma isolation and identification was performed on 30 lung tissue samples and 16 nasal swabs (HRM-culturing coincidence rate 87.0%; 40/46). HRM analysis was more sensitive than fluorescence qPCR and Mycoplasma isolation, indicating the practicality of HRM for accurate and rapid identification of Mccp and Mmc, and diagnosis and epidemiology of CCPP and MPGS.Jing-peng ZhangZhi-cheng LiuJin-xiu JiangYu-sheng LinWei YouQi-lin HuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jing-peng Zhang Zhi-cheng Liu Jin-xiu Jiang Yu-sheng Lin Wei You Qi-lin Hu Rapid detection of Mycoplasma mycoides subsp. capri and Mycoplasma capricolum subsp. capripneumoniae using high-resolution melting curve analysis
description	Abstract Mycoplasma capricolum subsp.subsp. capripneumonia (Mccp) and Mycoplasma mycoides subsp.sbusp. capri (Mmc) cause caprine pleuropneumonia (CCPP) and mycoplasmal pneumonia in goats and sheep (MPGS), respectively. These diseases cannot be identified on clinical symptoms alone and it is laborious to distinguish them using biochemical methods. It is therefore important to establish a simple, rapid identification method for Mccp and Mmc. Here, we report a high-resolution melting (HRM) curve analysis using specific primers based on the Mmc 95010 strain MLC_0560 and Mccp F38 strain MCCPF38_00984 gene sequences. The method was highly specific with intra- and inter-batch coefficients of variation < 1%. The lower limit of detection for Mccp and Mmc was 55 copies/μL and 58 copies/μL, respectively. HRM and fluorescence qPCR results were compared using 106 nasal swabs and 47 lung tissue samples from goats (HRM-qPCR coincidence rate 94.8%; 145/153). Mycoplasma isolation and identification was performed on 30 lung tissue samples and 16 nasal swabs (HRM-culturing coincidence rate 87.0%; 40/46). HRM analysis was more sensitive than fluorescence qPCR and Mycoplasma isolation, indicating the practicality of HRM for accurate and rapid identification of Mccp and Mmc, and diagnosis and epidemiology of CCPP and MPGS.
format	article
author	Jing-peng Zhang Zhi-cheng Liu Jin-xiu Jiang Yu-sheng Lin Wei You Qi-lin Hu
author_facet	Jing-peng Zhang Zhi-cheng Liu Jin-xiu Jiang Yu-sheng Lin Wei You Qi-lin Hu
author_sort	Jing-peng Zhang
title	Rapid detection of Mycoplasma mycoides subsp. capri and Mycoplasma capricolum subsp. capripneumoniae using high-resolution melting curve analysis
title_short	Rapid detection of Mycoplasma mycoides subsp. capri and Mycoplasma capricolum subsp. capripneumoniae using high-resolution melting curve analysis
title_full	Rapid detection of Mycoplasma mycoides subsp. capri and Mycoplasma capricolum subsp. capripneumoniae using high-resolution melting curve analysis
title_fullStr	Rapid detection of Mycoplasma mycoides subsp. capri and Mycoplasma capricolum subsp. capripneumoniae using high-resolution melting curve analysis
title_full_unstemmed	Rapid detection of Mycoplasma mycoides subsp. capri and Mycoplasma capricolum subsp. capripneumoniae using high-resolution melting curve analysis
title_sort	rapid detection of mycoplasma mycoides subsp. capri and mycoplasma capricolum subsp. capripneumoniae using high-resolution melting curve analysis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e968a1046fee4b089d493df13191c421
work_keys_str_mv	AT jingpengzhang rapiddetectionofmycoplasmamycoidessubspcapriandmycoplasmacapricolumsubspcapripneumoniaeusinghighresolutionmeltingcurveanalysis AT zhichengliu rapiddetectionofmycoplasmamycoidessubspcapriandmycoplasmacapricolumsubspcapripneumoniaeusinghighresolutionmeltingcurveanalysis AT jinxiujiang rapiddetectionofmycoplasmamycoidessubspcapriandmycoplasmacapricolumsubspcapripneumoniaeusinghighresolutionmeltingcurveanalysis AT yushenglin rapiddetectionofmycoplasmamycoidessubspcapriandmycoplasmacapricolumsubspcapripneumoniaeusinghighresolutionmeltingcurveanalysis AT weiyou rapiddetectionofmycoplasmamycoidessubspcapriandmycoplasmacapricolumsubspcapripneumoniaeusinghighresolutionmeltingcurveanalysis AT qilinhu rapiddetectionofmycoplasmamycoidessubspcapriandmycoplasmacapricolumsubspcapripneumoniaeusinghighresolutionmeltingcurveanalysis
_version_	1718384088527667200

Rapid detection of Mycoplasma mycoides subsp. capri and Mycoplasma capricolum subsp. capripneumoniae using high-resolution melting curve analysis

Ejemplares similares