Human chemokines as antimicrobial peptides with direct parasiticidal effect on Leishmania mexicana in vitro.
Chemokines and chemokine receptor-mediated effects are important mediators of the immunological response and cure in human leishmaniasis. However, in addition to their signalling properties for leukocytes, many chemokines have also been shown to act directly as antimicrobial peptides on bacteria and...
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2013
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oai:doaj.org-article:e96ba3d053e04726b167c91ecc780bf42021-11-18T07:52:22ZHuman chemokines as antimicrobial peptides with direct parasiticidal effect on Leishmania mexicana in vitro.1932-620310.1371/journal.pone.0058129https://doaj.org/article/e96ba3d053e04726b167c91ecc780bf42013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23533582/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Chemokines and chemokine receptor-mediated effects are important mediators of the immunological response and cure in human leishmaniasis. However, in addition to their signalling properties for leukocytes, many chemokines have also been shown to act directly as antimicrobial peptides on bacteria and fungi. We screened ten human chemokines (CXCL2, CXCL6, CXCL8, CXCL9, CXCL10, CCL2, CCL3, CCL20, CCL27, CCL28) for antimicrobial effects on the promastigote form of the protozoan parasite Leishmania mexicana, and observed direct parasiticidal effects of several, CCL28 being the most potent. Damage to the plasma membrane integrity could be visualised by entrance of propidium iodide, as measured with flow cytometry, and by scanning electron microscopy, which showed morphological changes and aggregation of cells. The findings were in concordance with parasiticidal activity, measured by decreased mitochondrial activity in an MTT-assay. This is the first report of direct antimicrobial activity by chemokines on parasites. This component of immunity against Leishmania parasites identified here warrants further investigation that might lead to new insight in the mechanisms of human infection and/or new therapeutic approaches.Sara K SöbirkMatthias MörgelinArne EgestenPaul BatesOonagh ShannonMattias CollinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58129 (2013) |
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DOAJ |
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Medicine R Science Q |
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Medicine R Science Q Sara K Söbirk Matthias Mörgelin Arne Egesten Paul Bates Oonagh Shannon Mattias Collin Human chemokines as antimicrobial peptides with direct parasiticidal effect on Leishmania mexicana in vitro. |
| description |
Chemokines and chemokine receptor-mediated effects are important mediators of the immunological response and cure in human leishmaniasis. However, in addition to their signalling properties for leukocytes, many chemokines have also been shown to act directly as antimicrobial peptides on bacteria and fungi. We screened ten human chemokines (CXCL2, CXCL6, CXCL8, CXCL9, CXCL10, CCL2, CCL3, CCL20, CCL27, CCL28) for antimicrobial effects on the promastigote form of the protozoan parasite Leishmania mexicana, and observed direct parasiticidal effects of several, CCL28 being the most potent. Damage to the plasma membrane integrity could be visualised by entrance of propidium iodide, as measured with flow cytometry, and by scanning electron microscopy, which showed morphological changes and aggregation of cells. The findings were in concordance with parasiticidal activity, measured by decreased mitochondrial activity in an MTT-assay. This is the first report of direct antimicrobial activity by chemokines on parasites. This component of immunity against Leishmania parasites identified here warrants further investigation that might lead to new insight in the mechanisms of human infection and/or new therapeutic approaches. |
| format |
article |
| author |
Sara K Söbirk Matthias Mörgelin Arne Egesten Paul Bates Oonagh Shannon Mattias Collin |
| author_facet |
Sara K Söbirk Matthias Mörgelin Arne Egesten Paul Bates Oonagh Shannon Mattias Collin |
| author_sort |
Sara K Söbirk |
| title |
Human chemokines as antimicrobial peptides with direct parasiticidal effect on Leishmania mexicana in vitro. |
| title_short |
Human chemokines as antimicrobial peptides with direct parasiticidal effect on Leishmania mexicana in vitro. |
| title_full |
Human chemokines as antimicrobial peptides with direct parasiticidal effect on Leishmania mexicana in vitro. |
| title_fullStr |
Human chemokines as antimicrobial peptides with direct parasiticidal effect on Leishmania mexicana in vitro. |
| title_full_unstemmed |
Human chemokines as antimicrobial peptides with direct parasiticidal effect on Leishmania mexicana in vitro. |
| title_sort |
human chemokines as antimicrobial peptides with direct parasiticidal effect on leishmania mexicana in vitro. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/e96ba3d053e04726b167c91ecc780bf4 |
| work_keys_str_mv |
AT saraksobirk humanchemokinesasantimicrobialpeptideswithdirectparasiticidaleffectonleishmaniamexicanainvitro AT matthiasmorgelin humanchemokinesasantimicrobialpeptideswithdirectparasiticidaleffectonleishmaniamexicanainvitro AT arneegesten humanchemokinesasantimicrobialpeptideswithdirectparasiticidaleffectonleishmaniamexicanainvitro AT paulbates humanchemokinesasantimicrobialpeptideswithdirectparasiticidaleffectonleishmaniamexicanainvitro AT oonaghshannon humanchemokinesasantimicrobialpeptideswithdirectparasiticidaleffectonleishmaniamexicanainvitro AT mattiascollin humanchemokinesasantimicrobialpeptideswithdirectparasiticidaleffectonleishmaniamexicanainvitro |
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