Transcriptome dataset of HEK293T cells depleted of one of the subunits of the DNA-PK complex: Ku70, Ku80 or DNA-PKcs

DNA-PK is a heterotrimeric complex that consists of Ku70 (XRCC6), Ku80 (XRCC5) and DNA-PKcs (PRKDC) subunits. The complex is a major player in the repair of DNA double strand break (DSB) via the non-homologous end joining (NHEJ) pathway. This process requires all DNA-PK subunits, since Ku70/Ku80 het...

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Autores principales:	Andrey Anisenko, Olga Shadrina, Irina Garanina, Marina Gottikh
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Ku heterodimer DNA-dependent protein kinase Ku70 Ku80 DNA-PKcs NHEJ-related proteins Computer applications to medicine. Medical informatics R858-859.7 Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/e9767795b04843aa9e6632dd8725bbbe
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spelling	oai:doaj.org-article:e9767795b04843aa9e6632dd8725bbbe2021-11-22T04:26:57ZTranscriptome dataset of HEK293T cells depleted of one of the subunits of the DNA-PK complex: Ku70, Ku80 or DNA-PKcs2352-340910.1016/j.dib.2021.107596https://doaj.org/article/e9767795b04843aa9e6632dd8725bbbe2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2352340921008714https://doaj.org/toc/2352-3409DNA-PK is a heterotrimeric complex that consists of Ku70 (XRCC6), Ku80 (XRCC5) and DNA-PKcs (PRKDC) subunits. The complex is a major player in the repair of DNA double strand break (DSB) via the non-homologous end joining (NHEJ) pathway. This process requires all DNA-PK subunits, since Ku70/Ku80 heterodimer firstly binds to DNA ends at DSB and then recruits DNA-PKcs. Recruitment of the DNA-PKcs subunit to DSB leads to phosphorylation events near DSB and recruitment of other NHEJ-related proteins that restore DNA integrity. However, today a lot of evidence demonstrates participation of the DNA-PK components in other cellular processes, e.g. telomere length maintenance, transcription, metabolism regulation, cytosolic DNA sensing, apoptosis, cellular movement and adhesion. It is important to note that not all the subunits of the DNA-PK complex are necessary for these processes, and the largest number of independent functions has been shown for the Ku70/Ku80 heterodimer and especially the Ku70 subunit. To better understand the role of each DNA-PK subunit in the cell life, we have analyzed transcriptome changes in HEK293T cells depleted of Ku70, Ku80 or DNA-PKcs using NGS-sequencing. Here, for the first time, we present the data obtained from the transcriptome analysis.Andrey AnisenkoOlga ShadrinaIrina GaraninaMarina GottikhElsevierarticleKu heterodimerDNA-dependent protein kinaseKu70Ku80DNA-PKcsNHEJ-related proteinsComputer applications to medicine. Medical informaticsR858-859.7Science (General)Q1-390ENData in Brief, Vol 39, Iss , Pp 107596- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ku heterodimer DNA-dependent protein kinase Ku70 Ku80 DNA-PKcs NHEJ-related proteins Computer applications to medicine. Medical informatics R858-859.7 Science (General) Q1-390
spellingShingle	Ku heterodimer DNA-dependent protein kinase Ku70 Ku80 DNA-PKcs NHEJ-related proteins Computer applications to medicine. Medical informatics R858-859.7 Science (General) Q1-390 Andrey Anisenko Olga Shadrina Irina Garanina Marina Gottikh Transcriptome dataset of HEK293T cells depleted of one of the subunits of the DNA-PK complex: Ku70, Ku80 or DNA-PKcs
description	DNA-PK is a heterotrimeric complex that consists of Ku70 (XRCC6), Ku80 (XRCC5) and DNA-PKcs (PRKDC) subunits. The complex is a major player in the repair of DNA double strand break (DSB) via the non-homologous end joining (NHEJ) pathway. This process requires all DNA-PK subunits, since Ku70/Ku80 heterodimer firstly binds to DNA ends at DSB and then recruits DNA-PKcs. Recruitment of the DNA-PKcs subunit to DSB leads to phosphorylation events near DSB and recruitment of other NHEJ-related proteins that restore DNA integrity. However, today a lot of evidence demonstrates participation of the DNA-PK components in other cellular processes, e.g. telomere length maintenance, transcription, metabolism regulation, cytosolic DNA sensing, apoptosis, cellular movement and adhesion. It is important to note that not all the subunits of the DNA-PK complex are necessary for these processes, and the largest number of independent functions has been shown for the Ku70/Ku80 heterodimer and especially the Ku70 subunit. To better understand the role of each DNA-PK subunit in the cell life, we have analyzed transcriptome changes in HEK293T cells depleted of Ku70, Ku80 or DNA-PKcs using NGS-sequencing. Here, for the first time, we present the data obtained from the transcriptome analysis.
format	article
author	Andrey Anisenko Olga Shadrina Irina Garanina Marina Gottikh
author_facet	Andrey Anisenko Olga Shadrina Irina Garanina Marina Gottikh
author_sort	Andrey Anisenko
title	Transcriptome dataset of HEK293T cells depleted of one of the subunits of the DNA-PK complex: Ku70, Ku80 or DNA-PKcs
title_short	Transcriptome dataset of HEK293T cells depleted of one of the subunits of the DNA-PK complex: Ku70, Ku80 or DNA-PKcs
title_full	Transcriptome dataset of HEK293T cells depleted of one of the subunits of the DNA-PK complex: Ku70, Ku80 or DNA-PKcs
title_fullStr	Transcriptome dataset of HEK293T cells depleted of one of the subunits of the DNA-PK complex: Ku70, Ku80 or DNA-PKcs
title_full_unstemmed	Transcriptome dataset of HEK293T cells depleted of one of the subunits of the DNA-PK complex: Ku70, Ku80 or DNA-PKcs
title_sort	transcriptome dataset of hek293t cells depleted of one of the subunits of the dna-pk complex: ku70, ku80 or dna-pkcs
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/e9767795b04843aa9e6632dd8725bbbe
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Transcriptome dataset of HEK293T cells depleted of one of the subunits of the DNA-PK complex: Ku70, Ku80 or DNA-PKcs

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