PLK2 Plays an Essential Role in High D-Glucose-Induced Apoptosis, ROS Generation and Inflammation in Podocytes

Abstract Diabetic kidney disease (DKD) is a serious complication of hyperglycemia. Currently, there is no effective therapeutic intervention for DKD. In this study, we sought to provide a set of gene profile in diabetic kidneys. We identified 338 genes altered in diabetes-induced DKD glomeruli, and...

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Autores principales:	Hong-hong Zou, Ping-ping Yang, Tian-lun Huang, Xiao-xu Zheng, Gao-si Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e981644ef1744814a32d0e9dff842cd7
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spelling	oai:doaj.org-article:e981644ef1744814a32d0e9dff842cd72021-12-02T11:53:10ZPLK2 Plays an Essential Role in High D-Glucose-Induced Apoptosis, ROS Generation and Inflammation in Podocytes10.1038/s41598-017-00686-82045-2322https://doaj.org/article/e981644ef1744814a32d0e9dff842cd72017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00686-8https://doaj.org/toc/2045-2322Abstract Diabetic kidney disease (DKD) is a serious complication of hyperglycemia. Currently, there is no effective therapeutic intervention for DKD. In this study, we sought to provide a set of gene profile in diabetic kidneys. We identified 338 genes altered in diabetes-induced DKD glomeruli, and PLK2 exhibited the most dramatic change. Gene set enrichment analysis (GSEA) indicated multiple signaling pathways are involved DKD pathogenesis. Here, we investigated whether PLK2 contributes to podocyte dysfunction, a characteristic change in the development of DKD. High D-glucose (HDG) significantly increased PLK2 expression in mouse podocytes. Suppressing PLK2 attenuated HDG-induced apoptosis and inflammatory responses both in vitro and in vivo. NAC, an antioxidant reagent, rescued HDG and PLK2 overexpression-induced kidney injuries. In summary, we demonstrated that silencing PLK2 attenuates HDG-induced podocyte apoptosis and inflammation, which may serve as a future therapeutic target in DKD.Hong-hong ZouPing-ping YangTian-lun HuangXiao-xu ZhengGao-si XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hong-hong Zou Ping-ping Yang Tian-lun Huang Xiao-xu Zheng Gao-si Xu PLK2 Plays an Essential Role in High D-Glucose-Induced Apoptosis, ROS Generation and Inflammation in Podocytes
description	Abstract Diabetic kidney disease (DKD) is a serious complication of hyperglycemia. Currently, there is no effective therapeutic intervention for DKD. In this study, we sought to provide a set of gene profile in diabetic kidneys. We identified 338 genes altered in diabetes-induced DKD glomeruli, and PLK2 exhibited the most dramatic change. Gene set enrichment analysis (GSEA) indicated multiple signaling pathways are involved DKD pathogenesis. Here, we investigated whether PLK2 contributes to podocyte dysfunction, a characteristic change in the development of DKD. High D-glucose (HDG) significantly increased PLK2 expression in mouse podocytes. Suppressing PLK2 attenuated HDG-induced apoptosis and inflammatory responses both in vitro and in vivo. NAC, an antioxidant reagent, rescued HDG and PLK2 overexpression-induced kidney injuries. In summary, we demonstrated that silencing PLK2 attenuates HDG-induced podocyte apoptosis and inflammation, which may serve as a future therapeutic target in DKD.
format	article
author	Hong-hong Zou Ping-ping Yang Tian-lun Huang Xiao-xu Zheng Gao-si Xu
author_facet	Hong-hong Zou Ping-ping Yang Tian-lun Huang Xiao-xu Zheng Gao-si Xu
author_sort	Hong-hong Zou
title	PLK2 Plays an Essential Role in High D-Glucose-Induced Apoptosis, ROS Generation and Inflammation in Podocytes
title_short	PLK2 Plays an Essential Role in High D-Glucose-Induced Apoptosis, ROS Generation and Inflammation in Podocytes
title_full	PLK2 Plays an Essential Role in High D-Glucose-Induced Apoptosis, ROS Generation and Inflammation in Podocytes
title_fullStr	PLK2 Plays an Essential Role in High D-Glucose-Induced Apoptosis, ROS Generation and Inflammation in Podocytes
title_full_unstemmed	PLK2 Plays an Essential Role in High D-Glucose-Induced Apoptosis, ROS Generation and Inflammation in Podocytes
title_sort	plk2 plays an essential role in high d-glucose-induced apoptosis, ros generation and inflammation in podocytes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e981644ef1744814a32d0e9dff842cd7
work_keys_str_mv	AT honghongzou plk2playsanessentialroleinhighdglucoseinducedapoptosisrosgenerationandinflammationinpodocytes AT pingpingyang plk2playsanessentialroleinhighdglucoseinducedapoptosisrosgenerationandinflammationinpodocytes AT tianlunhuang plk2playsanessentialroleinhighdglucoseinducedapoptosisrosgenerationandinflammationinpodocytes AT xiaoxuzheng plk2playsanessentialroleinhighdglucoseinducedapoptosisrosgenerationandinflammationinpodocytes AT gaosixu plk2playsanessentialroleinhighdglucoseinducedapoptosisrosgenerationandinflammationinpodocytes
_version_	1718394900788019200

PLK2 Plays an Essential Role in High D-Glucose-Induced Apoptosis, ROS Generation and Inflammation in Podocytes

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