Complement C7 is Specifically Expressed in Mesangial Cells and is a Potential Diagnostic Biomarker for Diabetic Nephropathy and is Regulated by miR-494-3p and miR-574-5p

Hang Guo,1 Zhiyue Yan,1 Yonghui Hu,1 Xitong Huang,2 Congqing Pan1 1NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin, 300134, People’s Republic of C...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Guo H, Yan Z, Hu Y, Huang X, Pan C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	diabetic nephropathy mesangial (mes) cells complement and coagulation cascades complement c7 mir-494-3p mir-574-5p biomarker. Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/e983c0698d8743ed83b2b18fbb093ec0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e983c0698d8743ed83b2b18fbb093ec0
record_format	dspace
spelling	oai:doaj.org-article:e983c0698d8743ed83b2b18fbb093ec02021-12-02T18:17:26ZComplement C7 is Specifically Expressed in Mesangial Cells and is a Potential Diagnostic Biomarker for Diabetic Nephropathy and is Regulated by miR-494-3p and miR-574-5p1178-7007https://doaj.org/article/e983c0698d8743ed83b2b18fbb093ec02021-07-01T00:00:00Zhttps://www.dovepress.com/complement-c7-is-specifically-expressed-in-mesangial-cells-and-is-a-po-peer-reviewed-fulltext-article-DMSOhttps://doaj.org/toc/1178-7007Hang Guo,1 Zhiyue Yan,1 Yonghui Hu,1 Xitong Huang,2 Congqing Pan1 1NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin, 300134, People’s Republic of China; 2Department of TCM, China Pharmaceutical University, Nanjing, Jiangsu, 211100, People’s Republic of ChinaCorrespondence: Congqing Pan Email cpan@tmu.edu.cnBackground: Diabetic nephropathy (DN) is the leading cause of end-stage renal disease, but it remains relatively underdiagnosed.Objective: In this study, we aimed to explore the key regulatory pathways and potential biomarkers related to DN using integrated bioinformatics analysis and validation.Methods: First, the microarray data of the GSE30528 and GSE96804 datasets were downloaded from the Gene Expression Omnibus (GEO) database, and differentially expressed genes (DEGs) were screened. Then, weighted gene coexpression network analysis (WGCNA), gene ontology (GO) annotation, gene set enrichment analysis (GSEA) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to identify key pathways and genes. qRT-PCR and receiver operating characteristic (ROC) curves were used to validate our results. Furthermore, single-cell RNA sequencing (scRNA-seq) data were reanalyzed to investigate the expression specificity of C7 in DN cells. An online database search and luciferase reporter assay identified the target relationship between miRNAs and C7.Results: The “complement and coagulation cascades” were significantly enriched, and complement C3 and C7 were candidate markers. The receiver operating characteristic (ROC) curve revealed that C7 had significant diagnostic value (AUC=0.865) in DN. Through scRNA-seq reanalysis, we found that C7 was specifically elevated in mesangial (MES) cells of DN. Moreover, we found that the expression of C7 was regulated by miR-494-3p and miR-574-5p.Conclusion: This is the first study to reveal that C7 is specifically expressed in mesangial cells, is a potential diagnostic biomarker for diabetic nephropathy, and is regulated by miR-494-3p and miR-574-5p.Keywords: diabetic nephropathy, mesangial (MES) cells, complement and coagulation cascades, complement C7, miR-494-3p, miR-574-5p, biomarkerGuo HYan ZHu YHuang XPan CDove Medical Pressarticlediabetic nephropathymesangial (mes) cellscomplement and coagulation cascadescomplement c7mir-494-3pmir-574-5pbiomarker.Specialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 14, Pp 3077-3088 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diabetic nephropathy mesangial (mes) cells complement and coagulation cascades complement c7 mir-494-3p mir-574-5p biomarker. Specialties of internal medicine RC581-951
spellingShingle	diabetic nephropathy mesangial (mes) cells complement and coagulation cascades complement c7 mir-494-3p mir-574-5p biomarker. Specialties of internal medicine RC581-951 Guo H Yan Z Hu Y Huang X Pan C Complement C7 is Specifically Expressed in Mesangial Cells and is a Potential Diagnostic Biomarker for Diabetic Nephropathy and is Regulated by miR-494-3p and miR-574-5p
description	Hang Guo,1 Zhiyue Yan,1 Yonghui Hu,1 Xitong Huang,2 Congqing Pan1 1NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin, 300134, People’s Republic of China; 2Department of TCM, China Pharmaceutical University, Nanjing, Jiangsu, 211100, People’s Republic of ChinaCorrespondence: Congqing Pan Email cpan@tmu.edu.cnBackground: Diabetic nephropathy (DN) is the leading cause of end-stage renal disease, but it remains relatively underdiagnosed.Objective: In this study, we aimed to explore the key regulatory pathways and potential biomarkers related to DN using integrated bioinformatics analysis and validation.Methods: First, the microarray data of the GSE30528 and GSE96804 datasets were downloaded from the Gene Expression Omnibus (GEO) database, and differentially expressed genes (DEGs) were screened. Then, weighted gene coexpression network analysis (WGCNA), gene ontology (GO) annotation, gene set enrichment analysis (GSEA) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to identify key pathways and genes. qRT-PCR and receiver operating characteristic (ROC) curves were used to validate our results. Furthermore, single-cell RNA sequencing (scRNA-seq) data were reanalyzed to investigate the expression specificity of C7 in DN cells. An online database search and luciferase reporter assay identified the target relationship between miRNAs and C7.Results: The “complement and coagulation cascades” were significantly enriched, and complement C3 and C7 were candidate markers. The receiver operating characteristic (ROC) curve revealed that C7 had significant diagnostic value (AUC=0.865) in DN. Through scRNA-seq reanalysis, we found that C7 was specifically elevated in mesangial (MES) cells of DN. Moreover, we found that the expression of C7 was regulated by miR-494-3p and miR-574-5p.Conclusion: This is the first study to reveal that C7 is specifically expressed in mesangial cells, is a potential diagnostic biomarker for diabetic nephropathy, and is regulated by miR-494-3p and miR-574-5p.Keywords: diabetic nephropathy, mesangial (MES) cells, complement and coagulation cascades, complement C7, miR-494-3p, miR-574-5p, biomarker
format	article
author	Guo H Yan Z Hu Y Huang X Pan C
author_facet	Guo H Yan Z Hu Y Huang X Pan C
author_sort	Guo H
title	Complement C7 is Specifically Expressed in Mesangial Cells and is a Potential Diagnostic Biomarker for Diabetic Nephropathy and is Regulated by miR-494-3p and miR-574-5p
title_short	Complement C7 is Specifically Expressed in Mesangial Cells and is a Potential Diagnostic Biomarker for Diabetic Nephropathy and is Regulated by miR-494-3p and miR-574-5p
title_full	Complement C7 is Specifically Expressed in Mesangial Cells and is a Potential Diagnostic Biomarker for Diabetic Nephropathy and is Regulated by miR-494-3p and miR-574-5p
title_fullStr	Complement C7 is Specifically Expressed in Mesangial Cells and is a Potential Diagnostic Biomarker for Diabetic Nephropathy and is Regulated by miR-494-3p and miR-574-5p
title_full_unstemmed	Complement C7 is Specifically Expressed in Mesangial Cells and is a Potential Diagnostic Biomarker for Diabetic Nephropathy and is Regulated by miR-494-3p and miR-574-5p
title_sort	complement c7 is specifically expressed in mesangial cells and is a potential diagnostic biomarker for diabetic nephropathy and is regulated by mir-494-3p and mir-574-5p
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/e983c0698d8743ed83b2b18fbb093ec0
work_keys_str_mv	AT guoh complementc7isspecificallyexpressedinmesangialcellsandisapotentialdiagnosticbiomarkerfordiabeticnephropathyandisregulatedbymir4943pandmir5745p AT yanz complementc7isspecificallyexpressedinmesangialcellsandisapotentialdiagnosticbiomarkerfordiabeticnephropathyandisregulatedbymir4943pandmir5745p AT huy complementc7isspecificallyexpressedinmesangialcellsandisapotentialdiagnosticbiomarkerfordiabeticnephropathyandisregulatedbymir4943pandmir5745p AT huangx complementc7isspecificallyexpressedinmesangialcellsandisapotentialdiagnosticbiomarkerfordiabeticnephropathyandisregulatedbymir4943pandmir5745p AT panc complementc7isspecificallyexpressedinmesangialcellsandisapotentialdiagnosticbiomarkerfordiabeticnephropathyandisregulatedbymir4943pandmir5745p
_version_	1718378306466742272

Complement C7 is Specifically Expressed in Mesangial Cells and is a Potential Diagnostic Biomarker for Diabetic Nephropathy and is Regulated by miR-494-3p and miR-574-5p

Ejemplares similares