Local dimensionality determines imaging speed in localization microscopy

Localisation microscopy enables nanometre-scale imaging of biological samples, but the method is too slow to use on dynamic systems. Here, the authors develop a mathematical model that optimises the number of frames required and estimates the maximum speed for super-resolution imaging.

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Bibliographic Details
Main Authors: Patrick Fox-Roberts, Richard Marsh, Karin Pfisterer, Asier Jayo, Maddy Parsons, Susan Cox
Format: article
Language:EN
Published: Nature Portfolio 2017
Subjects:
Q
Online Access:https://doaj.org/article/e984f0423c1e4fbda3a2068a71ae849a
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Summary:Localisation microscopy enables nanometre-scale imaging of biological samples, but the method is too slow to use on dynamic systems. Here, the authors develop a mathematical model that optimises the number of frames required and estimates the maximum speed for super-resolution imaging.