Local dimensionality determines imaging speed in localization microscopy
Localisation microscopy enables nanometre-scale imaging of biological samples, but the method is too slow to use on dynamic systems. Here, the authors develop a mathematical model that optimises the number of frames required and estimates the maximum speed for super-resolution imaging.
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Nature Portfolio
2017
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oai:doaj.org-article:e984f0423c1e4fbda3a2068a71ae849a2021-12-02T15:38:49ZLocal dimensionality determines imaging speed in localization microscopy10.1038/ncomms135582041-1723https://doaj.org/article/e984f0423c1e4fbda3a2068a71ae849a2017-01-01T00:00:00Zhttps://doi.org/10.1038/ncomms13558https://doaj.org/toc/2041-1723Localisation microscopy enables nanometre-scale imaging of biological samples, but the method is too slow to use on dynamic systems. Here, the authors develop a mathematical model that optimises the number of frames required and estimates the maximum speed for super-resolution imaging.Patrick Fox-RobertsRichard MarshKarin PfistererAsier JayoMaddy ParsonsSusan CoxNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-10 (2017) |
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Science Q Patrick Fox-Roberts Richard Marsh Karin Pfisterer Asier Jayo Maddy Parsons Susan Cox Local dimensionality determines imaging speed in localization microscopy |
description |
Localisation microscopy enables nanometre-scale imaging of biological samples, but the method is too slow to use on dynamic systems. Here, the authors develop a mathematical model that optimises the number of frames required and estimates the maximum speed for super-resolution imaging. |
format |
article |
author |
Patrick Fox-Roberts Richard Marsh Karin Pfisterer Asier Jayo Maddy Parsons Susan Cox |
author_facet |
Patrick Fox-Roberts Richard Marsh Karin Pfisterer Asier Jayo Maddy Parsons Susan Cox |
author_sort |
Patrick Fox-Roberts |
title |
Local dimensionality determines imaging speed in localization microscopy |
title_short |
Local dimensionality determines imaging speed in localization microscopy |
title_full |
Local dimensionality determines imaging speed in localization microscopy |
title_fullStr |
Local dimensionality determines imaging speed in localization microscopy |
title_full_unstemmed |
Local dimensionality determines imaging speed in localization microscopy |
title_sort |
local dimensionality determines imaging speed in localization microscopy |
publisher |
Nature Portfolio |
publishDate |
2017 |
url |
https://doaj.org/article/e984f0423c1e4fbda3a2068a71ae849a |
work_keys_str_mv |
AT patrickfoxroberts localdimensionalitydeterminesimagingspeedinlocalizationmicroscopy AT richardmarsh localdimensionalitydeterminesimagingspeedinlocalizationmicroscopy AT karinpfisterer localdimensionalitydeterminesimagingspeedinlocalizationmicroscopy AT asierjayo localdimensionalitydeterminesimagingspeedinlocalizationmicroscopy AT maddyparsons localdimensionalitydeterminesimagingspeedinlocalizationmicroscopy AT susancox localdimensionalitydeterminesimagingspeedinlocalizationmicroscopy |
_version_ |
1718386082592063488 |