Electronic cigarette smoke reduces ribosomal protein gene expression to impair protein synthesis in primary human airway epithelial cells
Abstract The widespread use of electronic cigarettes (e-cig) is a serious public health concern; however, mechanisms by which e-cig impair the function of airway epithelial cells—the direct target of e-cig smoke—are not fully understood. Here we report transcriptomic changes, including decreased exp...
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Nature Portfolio
2021
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oai:doaj.org-article:e98fd73566834d71945540b957f892982021-12-02T16:37:38ZElectronic cigarette smoke reduces ribosomal protein gene expression to impair protein synthesis in primary human airway epithelial cells10.1038/s41598-021-97013-z2045-2322https://doaj.org/article/e98fd73566834d71945540b957f892982021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97013-zhttps://doaj.org/toc/2045-2322Abstract The widespread use of electronic cigarettes (e-cig) is a serious public health concern; however, mechanisms by which e-cig impair the function of airway epithelial cells—the direct target of e-cig smoke—are not fully understood. Here we report transcriptomic changes, including decreased expression of many ribosomal genes, in airway epithelial cells in response to e-cig exposure. Using RNA-seq we identify over 200 differentially expressed genes in air–liquid interface cultured primary normal human bronchial epithelial (NHBE) exposed to e-cig smoke solution from commercial e-cig cartridges. In particular, exposure to e-cig smoke solution inhibits biological pathways involving ribosomes and protein biogenesis in NHBE cells. Consistent with this effect, expression of corresponding ribosomal proteins and subsequent protein biogenesis are reduced in the cells exposed to e-cig. Gas chromatography/mass spectrometry (GC/MS) analysis identified the presence of five flavoring chemicals designated as ‘high priority’ in regard to respiratory health, and methylglyoxal in e-cig smoke solution. Together, our findings reveal the potential detrimental effect of e-cig smoke on ribosomes and the associated protein biogenesis in airway epithelium. Our study calls for further investigation into how these changes in the airway epithelium contribute to the current epidemic of lung injuries in e-cig users.Hae-Ryung ParkJose VallarinoMichael O’SullivanCharlotte WirthRonald A. PanganibanGabrielle WebbMaya ShumyatcherBlanca E. HimesJin-Ah ParkDavid C. ChristianiJoseph AllenQuan LuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
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Medicine R Science Q Hae-Ryung Park Jose Vallarino Michael O’Sullivan Charlotte Wirth Ronald A. Panganiban Gabrielle Webb Maya Shumyatcher Blanca E. Himes Jin-Ah Park David C. Christiani Joseph Allen Quan Lu Electronic cigarette smoke reduces ribosomal protein gene expression to impair protein synthesis in primary human airway epithelial cells |
| description |
Abstract The widespread use of electronic cigarettes (e-cig) is a serious public health concern; however, mechanisms by which e-cig impair the function of airway epithelial cells—the direct target of e-cig smoke—are not fully understood. Here we report transcriptomic changes, including decreased expression of many ribosomal genes, in airway epithelial cells in response to e-cig exposure. Using RNA-seq we identify over 200 differentially expressed genes in air–liquid interface cultured primary normal human bronchial epithelial (NHBE) exposed to e-cig smoke solution from commercial e-cig cartridges. In particular, exposure to e-cig smoke solution inhibits biological pathways involving ribosomes and protein biogenesis in NHBE cells. Consistent with this effect, expression of corresponding ribosomal proteins and subsequent protein biogenesis are reduced in the cells exposed to e-cig. Gas chromatography/mass spectrometry (GC/MS) analysis identified the presence of five flavoring chemicals designated as ‘high priority’ in regard to respiratory health, and methylglyoxal in e-cig smoke solution. Together, our findings reveal the potential detrimental effect of e-cig smoke on ribosomes and the associated protein biogenesis in airway epithelium. Our study calls for further investigation into how these changes in the airway epithelium contribute to the current epidemic of lung injuries in e-cig users. |
| format |
article |
| author |
Hae-Ryung Park Jose Vallarino Michael O’Sullivan Charlotte Wirth Ronald A. Panganiban Gabrielle Webb Maya Shumyatcher Blanca E. Himes Jin-Ah Park David C. Christiani Joseph Allen Quan Lu |
| author_facet |
Hae-Ryung Park Jose Vallarino Michael O’Sullivan Charlotte Wirth Ronald A. Panganiban Gabrielle Webb Maya Shumyatcher Blanca E. Himes Jin-Ah Park David C. Christiani Joseph Allen Quan Lu |
| author_sort |
Hae-Ryung Park |
| title |
Electronic cigarette smoke reduces ribosomal protein gene expression to impair protein synthesis in primary human airway epithelial cells |
| title_short |
Electronic cigarette smoke reduces ribosomal protein gene expression to impair protein synthesis in primary human airway epithelial cells |
| title_full |
Electronic cigarette smoke reduces ribosomal protein gene expression to impair protein synthesis in primary human airway epithelial cells |
| title_fullStr |
Electronic cigarette smoke reduces ribosomal protein gene expression to impair protein synthesis in primary human airway epithelial cells |
| title_full_unstemmed |
Electronic cigarette smoke reduces ribosomal protein gene expression to impair protein synthesis in primary human airway epithelial cells |
| title_sort |
electronic cigarette smoke reduces ribosomal protein gene expression to impair protein synthesis in primary human airway epithelial cells |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/e98fd73566834d71945540b957f89298 |
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