Aging affects artemisinin synthesis in Artemisia annua

Abstract Artemisinin (ART) is the most effective component in malaria treatment, however, the extremely low content restricts its clinical application. Therefore, it is urgent to increase the yield of ART. ART gradually accumulates with aging, small RNA (sRNA) and transcriptome analysis were applied...

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Autores principales:	Jiao Li, Xiao-Hui Chu, Xiao-Yu Wang, Bao-Min Feng, Zong-Xia Yu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e9910cf7642949b9be50d70223099204
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spelling	oai:doaj.org-article:e9910cf7642949b9be50d702230992042021-12-02T14:47:37ZAging affects artemisinin synthesis in Artemisia annua10.1038/s41598-021-90807-12045-2322https://doaj.org/article/e9910cf7642949b9be50d702230992042021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90807-1https://doaj.org/toc/2045-2322Abstract Artemisinin (ART) is the most effective component in malaria treatment, however, the extremely low content restricts its clinical application. Therefore, it is urgent to increase the yield of ART. ART gradually accumulates with aging, small RNA (sRNA) and transcriptome analysis were applied on the leaves of 2-week-old (2 w) and 3-month-old (3 m) A. annua respectively. Among all the annotated sRNAs, 125 were upregulated and 128 downregulated in the 3 m sample compared to the 2 w one. Whereas 2183 genes were upregulated and 2156 downregulated. Notably, the level of miR156 and several annotated miRNAs gradually decreased while SPLs increased. In addition, the genes on ART biosynthesis pathway were significantly upregulated including ADS, CYP71AV1, ADH1, DBR2 and ALDH1, and so were the positive transcription factors like AaERF1, AaORA and AaWRKY1 indicating that age influences the ART biosynthesis by activating the expression of the synthesizing genes as well as positive transcription factors. This study contributes to reveal the regulatory effects of age on ART biosynthesis both in sRNA and transcription levels.Jiao LiXiao-Hui ChuXiao-Yu WangBao-Min FengZong-Xia YuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jiao Li Xiao-Hui Chu Xiao-Yu Wang Bao-Min Feng Zong-Xia Yu Aging affects artemisinin synthesis in Artemisia annua
description	Abstract Artemisinin (ART) is the most effective component in malaria treatment, however, the extremely low content restricts its clinical application. Therefore, it is urgent to increase the yield of ART. ART gradually accumulates with aging, small RNA (sRNA) and transcriptome analysis were applied on the leaves of 2-week-old (2 w) and 3-month-old (3 m) A. annua respectively. Among all the annotated sRNAs, 125 were upregulated and 128 downregulated in the 3 m sample compared to the 2 w one. Whereas 2183 genes were upregulated and 2156 downregulated. Notably, the level of miR156 and several annotated miRNAs gradually decreased while SPLs increased. In addition, the genes on ART biosynthesis pathway were significantly upregulated including ADS, CYP71AV1, ADH1, DBR2 and ALDH1, and so were the positive transcription factors like AaERF1, AaORA and AaWRKY1 indicating that age influences the ART biosynthesis by activating the expression of the synthesizing genes as well as positive transcription factors. This study contributes to reveal the regulatory effects of age on ART biosynthesis both in sRNA and transcription levels.
format	article
author	Jiao Li Xiao-Hui Chu Xiao-Yu Wang Bao-Min Feng Zong-Xia Yu
author_facet	Jiao Li Xiao-Hui Chu Xiao-Yu Wang Bao-Min Feng Zong-Xia Yu
author_sort	Jiao Li
title	Aging affects artemisinin synthesis in Artemisia annua
title_short	Aging affects artemisinin synthesis in Artemisia annua
title_full	Aging affects artemisinin synthesis in Artemisia annua
title_fullStr	Aging affects artemisinin synthesis in Artemisia annua
title_full_unstemmed	Aging affects artemisinin synthesis in Artemisia annua
title_sort	aging affects artemisinin synthesis in artemisia annua
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e9910cf7642949b9be50d70223099204
work_keys_str_mv	AT jiaoli agingaffectsartemisininsynthesisinartemisiaannua AT xiaohuichu agingaffectsartemisininsynthesisinartemisiaannua AT xiaoyuwang agingaffectsartemisininsynthesisinartemisiaannua AT baominfeng agingaffectsartemisininsynthesisinartemisiaannua AT zongxiayu agingaffectsartemisininsynthesisinartemisiaannua
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Aging affects artemisinin synthesis in Artemisia annua

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