Application of Metabolomics in the Study of Starvation-Induced Autophagy in <i>Saccharomyces cerevisiae</i>: A Scoping Review

This scoping review is aimed at the application of the metabolomics platform to dissect key metabolites and their intermediates to observe the regulatory mechanisms of starvation-induced autophagy in <i>Saccharomyces cerevisiae</i>. Four research papers were shortlisted in this review fo...

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Autores principales:	Muhammad Luqman Nasaruddin, Khaizurin Tajul Arifin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	autophagy starvation metabolomic metabolites <i>Saccharomyces cerevisiae</i> yeast Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e99448098a1a4a4a9a14e297506e0f8b
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spelling	oai:doaj.org-article:e99448098a1a4a4a9a14e297506e0f8b2021-11-25T18:06:34ZApplication of Metabolomics in the Study of Starvation-Induced Autophagy in <i>Saccharomyces cerevisiae</i>: A Scoping Review10.3390/jof71109872309-608Xhttps://doaj.org/article/e99448098a1a4a4a9a14e297506e0f8b2021-11-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/987https://doaj.org/toc/2309-608XThis scoping review is aimed at the application of the metabolomics platform to dissect key metabolites and their intermediates to observe the regulatory mechanisms of starvation-induced autophagy in <i>Saccharomyces cerevisiae</i>. Four research papers were shortlisted in this review following the inclusion and exclusion criteria. We observed a commonly shared pathway undertaken by <i>S. cerevisiae</i> under nutritional stress. Targeted and untargeted metabolomics was applied in either of these studies using varying platforms resulting in the annotation of several different observable metabolites. We saw a commonly shared pathway undertaken by <i>S. cerevisiae</i> under nutritional stress. Following nitrogen starvation, the concentration of cellular nucleosides was altered as a result of autophagic RNA degradation. Additionally, it is also found that autophagy replenishes amino acid pools to sustain macromolecule synthesis. Furthermore, in glucose starvation, nucleosides were broken down into carbonaceous metabolites that are being funneled into the non-oxidative pentose phosphate pathway. The ribose salvage allows for the survival of starved yeast. Moreover, acute glucose starvation showed autophagy to be involved in maintaining ATP/energy levels. We highlighted the practicality of metabolomics as a tool to better understand the underlying mechanisms involved to maintain homeostasis by recycling degradative products to ensure the survival of <i>S. cerevisiae</i> under starvation. The application of metabolomics has extended the scope of autophagy and provided newer intervention targets against cancer as well as neurodegenerative diseases in which autophagy is implicated.Muhammad Luqman NasaruddinKhaizurin Tajul ArifinMDPI AGarticleautophagystarvationmetabolomicmetabolites<i>Saccharomyces cerevisiae</i>yeastBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 987, p 987 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	autophagy starvation metabolomic metabolites <i>Saccharomyces cerevisiae</i> yeast Biology (General) QH301-705.5
spellingShingle	autophagy starvation metabolomic metabolites <i>Saccharomyces cerevisiae</i> yeast Biology (General) QH301-705.5 Muhammad Luqman Nasaruddin Khaizurin Tajul Arifin Application of Metabolomics in the Study of Starvation-Induced Autophagy in <i>Saccharomyces cerevisiae</i>: A Scoping Review
description	This scoping review is aimed at the application of the metabolomics platform to dissect key metabolites and their intermediates to observe the regulatory mechanisms of starvation-induced autophagy in <i>Saccharomyces cerevisiae</i>. Four research papers were shortlisted in this review following the inclusion and exclusion criteria. We observed a commonly shared pathway undertaken by <i>S. cerevisiae</i> under nutritional stress. Targeted and untargeted metabolomics was applied in either of these studies using varying platforms resulting in the annotation of several different observable metabolites. We saw a commonly shared pathway undertaken by <i>S. cerevisiae</i> under nutritional stress. Following nitrogen starvation, the concentration of cellular nucleosides was altered as a result of autophagic RNA degradation. Additionally, it is also found that autophagy replenishes amino acid pools to sustain macromolecule synthesis. Furthermore, in glucose starvation, nucleosides were broken down into carbonaceous metabolites that are being funneled into the non-oxidative pentose phosphate pathway. The ribose salvage allows for the survival of starved yeast. Moreover, acute glucose starvation showed autophagy to be involved in maintaining ATP/energy levels. We highlighted the practicality of metabolomics as a tool to better understand the underlying mechanisms involved to maintain homeostasis by recycling degradative products to ensure the survival of <i>S. cerevisiae</i> under starvation. The application of metabolomics has extended the scope of autophagy and provided newer intervention targets against cancer as well as neurodegenerative diseases in which autophagy is implicated.
format	article
author	Muhammad Luqman Nasaruddin Khaizurin Tajul Arifin
author_facet	Muhammad Luqman Nasaruddin Khaizurin Tajul Arifin
author_sort	Muhammad Luqman Nasaruddin
title	Application of Metabolomics in the Study of Starvation-Induced Autophagy in <i>Saccharomyces cerevisiae</i>: A Scoping Review
title_short	Application of Metabolomics in the Study of Starvation-Induced Autophagy in <i>Saccharomyces cerevisiae</i>: A Scoping Review
title_full	Application of Metabolomics in the Study of Starvation-Induced Autophagy in <i>Saccharomyces cerevisiae</i>: A Scoping Review
title_fullStr	Application of Metabolomics in the Study of Starvation-Induced Autophagy in <i>Saccharomyces cerevisiae</i>: A Scoping Review
title_full_unstemmed	Application of Metabolomics in the Study of Starvation-Induced Autophagy in <i>Saccharomyces cerevisiae</i>: A Scoping Review
title_sort	application of metabolomics in the study of starvation-induced autophagy in <i>saccharomyces cerevisiae</i>: a scoping review
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e99448098a1a4a4a9a14e297506e0f8b
work_keys_str_mv	AT muhammadluqmannasaruddin applicationofmetabolomicsinthestudyofstarvationinducedautophagyinisaccharomycescerevisiaeiascopingreview AT khaizurintajularifin applicationofmetabolomicsinthestudyofstarvationinducedautophagyinisaccharomycescerevisiaeiascopingreview
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Application of Metabolomics in the Study of Starvation-Induced Autophagy in <i>Saccharomyces cerevisiae</i>: A Scoping Review

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