Using natural selection to explore the adaptive potential of Chlamydomonas reinhardtii.

Improving feedstock is critical to facilitate the commercial utilization of algae, in particular in open pond systems where, due to the presence of competitors and pests, high algal growth rates and stress tolerance are beneficial. Here we raised laboratory cultures of the model alga Chlamydomonas r...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marie-Mathilde Perrineau, Jeferson Gross, Ehud Zelzion, Dana C Price, Orly Levitan, Jeffrey Boyd, Debashish Bhattacharya
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e99520e253cc46a4b689ef5c27e15c37
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e99520e253cc46a4b689ef5c27e15c37
record_format	dspace
spelling	oai:doaj.org-article:e99520e253cc46a4b689ef5c27e15c372021-11-18T08:26:47ZUsing natural selection to explore the adaptive potential of Chlamydomonas reinhardtii.1932-620310.1371/journal.pone.0092533https://doaj.org/article/e99520e253cc46a4b689ef5c27e15c372014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24658261/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Improving feedstock is critical to facilitate the commercial utilization of algae, in particular in open pond systems where, due to the presence of competitors and pests, high algal growth rates and stress tolerance are beneficial. Here we raised laboratory cultures of the model alga Chlamydomonas reinhardtii under serial dilution to explore the potential of crop improvement using natural selection. The alga was evolved for 1,880 generations in liquid medium under continuous light (EL population). At the end of the experiment, EL cells had a growth rate that was 35% greater than the progenitor population (PL). The removal of acetate from the medium demonstrated that EL growth enhancement largely relied on efficient usage of this organic carbon source. Genome re-sequencing uncovered 1,937 polymorphic DNA regions in the EL population with 149 single nucleotide polymorphisms resulting in amino acid substitutions. Transcriptome analysis showed, in the EL population, significant up regulation of genes involved in protein synthesis, the cell cycle and cellular respiration, whereas the DNA repair pathway and photosynthesis were down regulated. Like other algae, EL cells accumulated neutral lipids under nitrogen depletion. Our work demonstrates transcriptome and genome-wide impacts of natural selection on algal cells and points to a useful strategy for strain improvement.Marie-Mathilde PerrineauJeferson GrossEhud ZelzionDana C PriceOrly LevitanJeffrey BoydDebashish BhattacharyaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e92533 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marie-Mathilde Perrineau Jeferson Gross Ehud Zelzion Dana C Price Orly Levitan Jeffrey Boyd Debashish Bhattacharya Using natural selection to explore the adaptive potential of Chlamydomonas reinhardtii.
description	Improving feedstock is critical to facilitate the commercial utilization of algae, in particular in open pond systems where, due to the presence of competitors and pests, high algal growth rates and stress tolerance are beneficial. Here we raised laboratory cultures of the model alga Chlamydomonas reinhardtii under serial dilution to explore the potential of crop improvement using natural selection. The alga was evolved for 1,880 generations in liquid medium under continuous light (EL population). At the end of the experiment, EL cells had a growth rate that was 35% greater than the progenitor population (PL). The removal of acetate from the medium demonstrated that EL growth enhancement largely relied on efficient usage of this organic carbon source. Genome re-sequencing uncovered 1,937 polymorphic DNA regions in the EL population with 149 single nucleotide polymorphisms resulting in amino acid substitutions. Transcriptome analysis showed, in the EL population, significant up regulation of genes involved in protein synthesis, the cell cycle and cellular respiration, whereas the DNA repair pathway and photosynthesis were down regulated. Like other algae, EL cells accumulated neutral lipids under nitrogen depletion. Our work demonstrates transcriptome and genome-wide impacts of natural selection on algal cells and points to a useful strategy for strain improvement.
format	article
author	Marie-Mathilde Perrineau Jeferson Gross Ehud Zelzion Dana C Price Orly Levitan Jeffrey Boyd Debashish Bhattacharya
author_facet	Marie-Mathilde Perrineau Jeferson Gross Ehud Zelzion Dana C Price Orly Levitan Jeffrey Boyd Debashish Bhattacharya
author_sort	Marie-Mathilde Perrineau
title	Using natural selection to explore the adaptive potential of Chlamydomonas reinhardtii.
title_short	Using natural selection to explore the adaptive potential of Chlamydomonas reinhardtii.
title_full	Using natural selection to explore the adaptive potential of Chlamydomonas reinhardtii.
title_fullStr	Using natural selection to explore the adaptive potential of Chlamydomonas reinhardtii.
title_full_unstemmed	Using natural selection to explore the adaptive potential of Chlamydomonas reinhardtii.
title_sort	using natural selection to explore the adaptive potential of chlamydomonas reinhardtii.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/e99520e253cc46a4b689ef5c27e15c37
work_keys_str_mv	AT mariemathildeperrineau usingnaturalselectiontoexploretheadaptivepotentialofchlamydomonasreinhardtii AT jefersongross usingnaturalselectiontoexploretheadaptivepotentialofchlamydomonasreinhardtii AT ehudzelzion usingnaturalselectiontoexploretheadaptivepotentialofchlamydomonasreinhardtii AT danacprice usingnaturalselectiontoexploretheadaptivepotentialofchlamydomonasreinhardtii AT orlylevitan usingnaturalselectiontoexploretheadaptivepotentialofchlamydomonasreinhardtii AT jeffreyboyd usingnaturalselectiontoexploretheadaptivepotentialofchlamydomonasreinhardtii AT debashishbhattacharya usingnaturalselectiontoexploretheadaptivepotentialofchlamydomonasreinhardtii
_version_	1718421833982672896

Using natural selection to explore the adaptive potential of Chlamydomonas reinhardtii.

Ejemplares similares