Inhibitory effects of fucoidan on NMDA receptors and l-type Ca2+ channels regulating the Ca2+ responses in rat neurons

Context: Fucoidan, a sulphated polysaccharide extracted from brown algae [Fucus vesiculosus Linn. (Fucaceae)], has multiple biological activities. Objective: The effects of fucoidan on Ca2+ responses of rat neurons and its probable mechanisms with focus on glutamate receptors were examined. Material...

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Autores principales:	Hong Wu, Shuibo Gao, Susumu Terakawa
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	cortical and hippocampal neuron ca2+ transient sulphated polysaccharide mrna expression of pr1/pr2 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/e99e2508d9cb4a8d9f6265ba562959b0
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spelling	oai:doaj.org-article:e99e2508d9cb4a8d9f6265ba562959b02021-11-17T14:21:55ZInhibitory effects of fucoidan on NMDA receptors and l-type Ca2+ channels regulating the Ca2+ responses in rat neurons1388-02091744-511610.1080/13880209.2018.1548626https://doaj.org/article/e99e2508d9cb4a8d9f6265ba562959b02019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2018.1548626https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Fucoidan, a sulphated polysaccharide extracted from brown algae [Fucus vesiculosus Linn. (Fucaceae)], has multiple biological activities. Objective: The effects of fucoidan on Ca2+ responses of rat neurons and its probable mechanisms with focus on glutamate receptors were examined. Materials and methods: The neurons isolated from the cortex and hippocampi of Wistar rats in postnatal day 1 were employed. The intracellular Ca2+ responses triggered by various stimuli were measured in vitro by Fura-2/AM. Fucoidan at 0.5 mg/mL or 1.5 mg/mL was applied for 3 min to determine its effects on Ca2+ responses. RT-PCR was used to determine the mRNA expression of neuron receptors treated with fucoidan at 0.5 mg/mL for 3 h. Results: The Ca2+ responses induced by NMDA were 100% suppressed by fucoidan, and those induced by Bay K8644 90% in the cortical neurons. However, fucoidan has no significant effect on the Ca2+ responses of cortical neurons induced by AMPA or quisqualate. Meanwhile, the Ca2+ responses of hippocampal neurons induced by glutamate, ACPD or adrenaline, showed only a slight decrease following fucoidan treatment. RT-PCR assays of cortical and hippocampal neurons showed that fucoidan treatment significantly decreased the mRNA expression of NMDA-NR1 receptor and the primer pair for l-type Ca2+ channels, PR1/PR2. Discussion and conclusions: Our data indicate that fucoidan suppresses the intracellular Ca2+ responses by selectively inhibiting NMDA receptors in cortical neurons and l-type Ca2+ channels in hippocampal neurons. A wide spectrum of fucoidan binding to cell membrane may be useful for designing a general purpose drug in future.Hong WuShuibo GaoSusumu TerakawaTaylor & Francis Grouparticlecortical and hippocampal neuronca2+ transientsulphated polysaccharidemrna expression of pr1/pr2Therapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 1-7 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	cortical and hippocampal neuron ca2+ transient sulphated polysaccharide mrna expression of pr1/pr2 Therapeutics. Pharmacology RM1-950
spellingShingle	cortical and hippocampal neuron ca2+ transient sulphated polysaccharide mrna expression of pr1/pr2 Therapeutics. Pharmacology RM1-950 Hong Wu Shuibo Gao Susumu Terakawa Inhibitory effects of fucoidan on NMDA receptors and l-type Ca2+ channels regulating the Ca2+ responses in rat neurons
description	Context: Fucoidan, a sulphated polysaccharide extracted from brown algae [Fucus vesiculosus Linn. (Fucaceae)], has multiple biological activities. Objective: The effects of fucoidan on Ca2+ responses of rat neurons and its probable mechanisms with focus on glutamate receptors were examined. Materials and methods: The neurons isolated from the cortex and hippocampi of Wistar rats in postnatal day 1 were employed. The intracellular Ca2+ responses triggered by various stimuli were measured in vitro by Fura-2/AM. Fucoidan at 0.5 mg/mL or 1.5 mg/mL was applied for 3 min to determine its effects on Ca2+ responses. RT-PCR was used to determine the mRNA expression of neuron receptors treated with fucoidan at 0.5 mg/mL for 3 h. Results: The Ca2+ responses induced by NMDA were 100% suppressed by fucoidan, and those induced by Bay K8644 90% in the cortical neurons. However, fucoidan has no significant effect on the Ca2+ responses of cortical neurons induced by AMPA or quisqualate. Meanwhile, the Ca2+ responses of hippocampal neurons induced by glutamate, ACPD or adrenaline, showed only a slight decrease following fucoidan treatment. RT-PCR assays of cortical and hippocampal neurons showed that fucoidan treatment significantly decreased the mRNA expression of NMDA-NR1 receptor and the primer pair for l-type Ca2+ channels, PR1/PR2. Discussion and conclusions: Our data indicate that fucoidan suppresses the intracellular Ca2+ responses by selectively inhibiting NMDA receptors in cortical neurons and l-type Ca2+ channels in hippocampal neurons. A wide spectrum of fucoidan binding to cell membrane may be useful for designing a general purpose drug in future.
format	article
author	Hong Wu Shuibo Gao Susumu Terakawa
author_facet	Hong Wu Shuibo Gao Susumu Terakawa
author_sort	Hong Wu
title	Inhibitory effects of fucoidan on NMDA receptors and l-type Ca2+ channels regulating the Ca2+ responses in rat neurons
title_short	Inhibitory effects of fucoidan on NMDA receptors and l-type Ca2+ channels regulating the Ca2+ responses in rat neurons
title_full	Inhibitory effects of fucoidan on NMDA receptors and l-type Ca2+ channels regulating the Ca2+ responses in rat neurons
title_fullStr	Inhibitory effects of fucoidan on NMDA receptors and l-type Ca2+ channels regulating the Ca2+ responses in rat neurons
title_full_unstemmed	Inhibitory effects of fucoidan on NMDA receptors and l-type Ca2+ channels regulating the Ca2+ responses in rat neurons
title_sort	inhibitory effects of fucoidan on nmda receptors and l-type ca2+ channels regulating the ca2+ responses in rat neurons
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/e99e2508d9cb4a8d9f6265ba562959b0
work_keys_str_mv	AT hongwu inhibitoryeffectsoffucoidanonnmdareceptorsandltypeca2channelsregulatingtheca2responsesinratneurons AT shuibogao inhibitoryeffectsoffucoidanonnmdareceptorsandltypeca2channelsregulatingtheca2responsesinratneurons AT susumuterakawa inhibitoryeffectsoffucoidanonnmdareceptorsandltypeca2channelsregulatingtheca2responsesinratneurons
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Inhibitory effects of fucoidan on NMDA receptors and l-type Ca2+ channels regulating the Ca2+ responses in rat neurons

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