Co-Inoculation of Bacillus spp. for Growth Promotion and Iron Fortification in Sorghum

Seven Bacillus spp. isolated from the marine water and the rhizosphere of the medicinal plant Coscinium fenestratum were studied to produce plant growth promotion (PGP) traits invitro. Among the seven isolates, MMRH22 and RHPR20 produced copio...

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Autores principales:	Mansani Manasa, Polapally Ravinder, Subramaniam Gopalakrishnan, Vadlamudi Srinivas, R. Z. Sayyed, Hesham Ali El Enshasy, Maizatulakmal Yahayu, Ali Tan Kee Zuan, Hazem S. Kassem, Bee Hameeda
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Bacillus</i> consortium sorghum plant growth promotion biofortification Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/e99eef6bf36747308a73b429115ffd42
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spelling	oai:doaj.org-article:e99eef6bf36747308a73b429115ffd422021-11-11T19:43:54ZCo-Inoculation of <i>Bacillus</i> spp. for Growth Promotion and Iron Fortification in Sorghum10.3390/su1321120912071-1050https://doaj.org/article/e99eef6bf36747308a73b429115ffd422021-11-01T00:00:00Zhttps://www.mdpi.com/2071-1050/13/21/12091https://doaj.org/toc/2071-1050Seven <i>Bacillus</i> spp. isolated from the marine water and the rhizosphere of the medicinal plant <i>Coscinium fenestratum</i> were studied to produce plant growth promotion (PGP) traits <i>invitro.</i> Among the seven isolates, MMRH22 and RHPR20 produced copious amounts of PGP traits. Based on the 16S rRNA sequence, the two potent bacterial isolates, RHPR20 and MMRH22, were identified as <i>Bacillus mojavensis</i> and <i>Bacillus cereus,</i> respectively. A compatibility test between the isolates RHPR20 and MMRH22 revealed they are compatible and can be used as a consortium. Both isolates were evaluated for the plant growth promotion and the biofortification of sorghum under greenhouse conditions. Treatments included the application of MMRH22, RHPR20, their consortium (RHPR20 + MMRH22), and an uninoculated control. Inoculation with bacterial cultures resulted in a significant increase in the plant height; the number of leaves; the leaf area; the root, shoot, and leaf weight; and the yield of sorghum at 30 and 60 days after sowing (DAS). The scanning electron micrograph of the sorghum plant roots revealed extensive colonization in the plants treated with the bacterial cultures compared to the uninoculated control. The sorghum grains obtained after final harvest were analyzed for their nutrient content by ICP–OES. The biofortification in sorghum grains was varied and was found to enhance the iron content up to 97%. This study revealed that treatments with microbial consortia enhance plant growth, yield, and iron content, which could combat nutrient deficiencies in plants and humans.Mansani ManasaPolapally RavinderSubramaniam GopalakrishnanVadlamudi SrinivasR. Z. SayyedHesham Ali El EnshasyMaizatulakmal YahayuAli Tan Kee ZuanHazem S. KassemBee HameedaMDPI AGarticle<i>Bacillus</i>consortiumsorghumplant growth promotionbiofortificationEnvironmental effects of industries and plantsTD194-195Renewable energy sourcesTJ807-830Environmental sciencesGE1-350ENSustainability, Vol 13, Iss 12091, p 12091 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Bacillus</i> consortium sorghum plant growth promotion biofortification Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350
spellingShingle	<i>Bacillus</i> consortium sorghum plant growth promotion biofortification Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350 Mansani Manasa Polapally Ravinder Subramaniam Gopalakrishnan Vadlamudi Srinivas R. Z. Sayyed Hesham Ali El Enshasy Maizatulakmal Yahayu Ali Tan Kee Zuan Hazem S. Kassem Bee Hameeda Co-Inoculation of <i>Bacillus</i> spp. for Growth Promotion and Iron Fortification in Sorghum
description	Seven <i>Bacillus</i> spp. isolated from the marine water and the rhizosphere of the medicinal plant <i>Coscinium fenestratum</i> were studied to produce plant growth promotion (PGP) traits <i>invitro.</i> Among the seven isolates, MMRH22 and RHPR20 produced copious amounts of PGP traits. Based on the 16S rRNA sequence, the two potent bacterial isolates, RHPR20 and MMRH22, were identified as <i>Bacillus mojavensis</i> and <i>Bacillus cereus,</i> respectively. A compatibility test between the isolates RHPR20 and MMRH22 revealed they are compatible and can be used as a consortium. Both isolates were evaluated for the plant growth promotion and the biofortification of sorghum under greenhouse conditions. Treatments included the application of MMRH22, RHPR20, their consortium (RHPR20 + MMRH22), and an uninoculated control. Inoculation with bacterial cultures resulted in a significant increase in the plant height; the number of leaves; the leaf area; the root, shoot, and leaf weight; and the yield of sorghum at 30 and 60 days after sowing (DAS). The scanning electron micrograph of the sorghum plant roots revealed extensive colonization in the plants treated with the bacterial cultures compared to the uninoculated control. The sorghum grains obtained after final harvest were analyzed for their nutrient content by ICP–OES. The biofortification in sorghum grains was varied and was found to enhance the iron content up to 97%. This study revealed that treatments with microbial consortia enhance plant growth, yield, and iron content, which could combat nutrient deficiencies in plants and humans.
format	article
author	Mansani Manasa Polapally Ravinder Subramaniam Gopalakrishnan Vadlamudi Srinivas R. Z. Sayyed Hesham Ali El Enshasy Maizatulakmal Yahayu Ali Tan Kee Zuan Hazem S. Kassem Bee Hameeda
author_facet	Mansani Manasa Polapally Ravinder Subramaniam Gopalakrishnan Vadlamudi Srinivas R. Z. Sayyed Hesham Ali El Enshasy Maizatulakmal Yahayu Ali Tan Kee Zuan Hazem S. Kassem Bee Hameeda
author_sort	Mansani Manasa
title	Co-Inoculation of <i>Bacillus</i> spp. for Growth Promotion and Iron Fortification in Sorghum
title_short	Co-Inoculation of <i>Bacillus</i> spp. for Growth Promotion and Iron Fortification in Sorghum
title_full	Co-Inoculation of <i>Bacillus</i> spp. for Growth Promotion and Iron Fortification in Sorghum
title_fullStr	Co-Inoculation of <i>Bacillus</i> spp. for Growth Promotion and Iron Fortification in Sorghum
title_full_unstemmed	Co-Inoculation of <i>Bacillus</i> spp. for Growth Promotion and Iron Fortification in Sorghum
title_sort	co-inoculation of <i>bacillus</i> spp. for growth promotion and iron fortification in sorghum
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e99eef6bf36747308a73b429115ffd42
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Co-Inoculation of <i>Bacillus</i> spp. for Growth Promotion and Iron Fortification in Sorghum

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