Melanopsin as a sleep modulator: circadian gating of the direct effects of light on sleep and altered sleep homeostasis in Opn4(-/-) mice.

Light influences sleep and alertness either indirectly through a well-characterized circadian pathway or directly through yet poorly understood mechanisms. Melanopsin (Opn4) is a retinal photopigment crucial for conveying nonvisual light information to the brain. Through extensive characterization o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jessica W Tsai, Jens Hannibal, Grace Hagiwara, Damien Colas, Elisabeth Ruppert, Norman F Ruby, H Craig Heller, Paul Franken, Patrice Bourgin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e99f480dd1e8416390caddee6237b1f4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e99f480dd1e8416390caddee6237b1f4
record_format	dspace
spelling	oai:doaj.org-article:e99f480dd1e8416390caddee6237b1f42021-11-25T05:34:09ZMelanopsin as a sleep modulator: circadian gating of the direct effects of light on sleep and altered sleep homeostasis in Opn4(-/-) mice.1544-91731545-788510.1371/journal.pbio.1000125https://doaj.org/article/e99f480dd1e8416390caddee6237b1f42009-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19513122/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Light influences sleep and alertness either indirectly through a well-characterized circadian pathway or directly through yet poorly understood mechanisms. Melanopsin (Opn4) is a retinal photopigment crucial for conveying nonvisual light information to the brain. Through extensive characterization of sleep and the electrocorticogram (ECoG) in melanopsin-deficient (Opn4(-/-)) mice under various light-dark (LD) schedules, we assessed the role of melanopsin in mediating the effects of light on sleep and ECoG activity. In control mice, a light pulse given during the habitual dark period readily induced sleep, whereas a dark pulse given during the habitual light period induced waking with pronounced theta (7-10 Hz) and gamma (40-70 Hz) activity, the ECoG correlates of alertness. In contrast, light failed to induce sleep in Opn4(-/-) mice, and the dark-pulse-induced increase in theta and gamma activity was delayed. A 24-h recording under a LD 1-hratio1-h schedule revealed that the failure to respond to light in Opn4(-/-) mice was restricted to the subjective dark period. Light induced c-Fos immunoreactivity in the suprachiasmatic nuclei (SCN) and in sleep-active ventrolateral preoptic (VLPO) neurons was importantly reduced in Opn4(-/-) mice, implicating both sleep-regulatory structures in the melanopsin-mediated effects of light. In addition to these acute light effects, Opn4(-/-) mice slept 1 h less during the 12-h light period of a LD 12ratio12 schedule owing to a lengthening of waking bouts. Despite this reduction in sleep time, ECoG delta power, a marker of sleep need, was decreased in Opn4(-/-) mice for most of the (subjective) dark period. Delta power reached after a 6-h sleep deprivation was similarly reduced in Opn4(-/-) mice. In mice, melanopsin's contribution to the direct effects of light on sleep is limited to the dark or active period, suggesting that at this circadian phase, melanopsin compensates for circadian variations in the photo sensitivity of other light-encoding pathways such as rod and cones. Our study, furthermore, demonstrates that lack of melanopsin alters sleep homeostasis. These findings call for a reevaluation of the role of light on mammalian physiology and behavior.Jessica W TsaiJens HannibalGrace HagiwaraDamien ColasElisabeth RuppertNorman F RubyH Craig HellerPaul FrankenPatrice BourginPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 7, Iss 6, p e1000125 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Jessica W Tsai Jens Hannibal Grace Hagiwara Damien Colas Elisabeth Ruppert Norman F Ruby H Craig Heller Paul Franken Patrice Bourgin Melanopsin as a sleep modulator: circadian gating of the direct effects of light on sleep and altered sleep homeostasis in Opn4(-/-) mice.
description	Light influences sleep and alertness either indirectly through a well-characterized circadian pathway or directly through yet poorly understood mechanisms. Melanopsin (Opn4) is a retinal photopigment crucial for conveying nonvisual light information to the brain. Through extensive characterization of sleep and the electrocorticogram (ECoG) in melanopsin-deficient (Opn4(-/-)) mice under various light-dark (LD) schedules, we assessed the role of melanopsin in mediating the effects of light on sleep and ECoG activity. In control mice, a light pulse given during the habitual dark period readily induced sleep, whereas a dark pulse given during the habitual light period induced waking with pronounced theta (7-10 Hz) and gamma (40-70 Hz) activity, the ECoG correlates of alertness. In contrast, light failed to induce sleep in Opn4(-/-) mice, and the dark-pulse-induced increase in theta and gamma activity was delayed. A 24-h recording under a LD 1-hratio1-h schedule revealed that the failure to respond to light in Opn4(-/-) mice was restricted to the subjective dark period. Light induced c-Fos immunoreactivity in the suprachiasmatic nuclei (SCN) and in sleep-active ventrolateral preoptic (VLPO) neurons was importantly reduced in Opn4(-/-) mice, implicating both sleep-regulatory structures in the melanopsin-mediated effects of light. In addition to these acute light effects, Opn4(-/-) mice slept 1 h less during the 12-h light period of a LD 12ratio12 schedule owing to a lengthening of waking bouts. Despite this reduction in sleep time, ECoG delta power, a marker of sleep need, was decreased in Opn4(-/-) mice for most of the (subjective) dark period. Delta power reached after a 6-h sleep deprivation was similarly reduced in Opn4(-/-) mice. In mice, melanopsin's contribution to the direct effects of light on sleep is limited to the dark or active period, suggesting that at this circadian phase, melanopsin compensates for circadian variations in the photo sensitivity of other light-encoding pathways such as rod and cones. Our study, furthermore, demonstrates that lack of melanopsin alters sleep homeostasis. These findings call for a reevaluation of the role of light on mammalian physiology and behavior.
format	article
author	Jessica W Tsai Jens Hannibal Grace Hagiwara Damien Colas Elisabeth Ruppert Norman F Ruby H Craig Heller Paul Franken Patrice Bourgin
author_facet	Jessica W Tsai Jens Hannibal Grace Hagiwara Damien Colas Elisabeth Ruppert Norman F Ruby H Craig Heller Paul Franken Patrice Bourgin
author_sort	Jessica W Tsai
title	Melanopsin as a sleep modulator: circadian gating of the direct effects of light on sleep and altered sleep homeostasis in Opn4(-/-) mice.
title_short	Melanopsin as a sleep modulator: circadian gating of the direct effects of light on sleep and altered sleep homeostasis in Opn4(-/-) mice.
title_full	Melanopsin as a sleep modulator: circadian gating of the direct effects of light on sleep and altered sleep homeostasis in Opn4(-/-) mice.
title_fullStr	Melanopsin as a sleep modulator: circadian gating of the direct effects of light on sleep and altered sleep homeostasis in Opn4(-/-) mice.
title_full_unstemmed	Melanopsin as a sleep modulator: circadian gating of the direct effects of light on sleep and altered sleep homeostasis in Opn4(-/-) mice.
title_sort	melanopsin as a sleep modulator: circadian gating of the direct effects of light on sleep and altered sleep homeostasis in opn4(-/-) mice.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/e99f480dd1e8416390caddee6237b1f4
work_keys_str_mv	AT jessicawtsai melanopsinasasleepmodulatorcircadiangatingofthedirecteffectsoflightonsleepandalteredsleephomeostasisinopn4mice AT jenshannibal melanopsinasasleepmodulatorcircadiangatingofthedirecteffectsoflightonsleepandalteredsleephomeostasisinopn4mice AT gracehagiwara melanopsinasasleepmodulatorcircadiangatingofthedirecteffectsoflightonsleepandalteredsleephomeostasisinopn4mice AT damiencolas melanopsinasasleepmodulatorcircadiangatingofthedirecteffectsoflightonsleepandalteredsleephomeostasisinopn4mice AT elisabethruppert melanopsinasasleepmodulatorcircadiangatingofthedirecteffectsoflightonsleepandalteredsleephomeostasisinopn4mice AT normanfruby melanopsinasasleepmodulatorcircadiangatingofthedirecteffectsoflightonsleepandalteredsleephomeostasisinopn4mice AT hcraigheller melanopsinasasleepmodulatorcircadiangatingofthedirecteffectsoflightonsleepandalteredsleephomeostasisinopn4mice AT paulfranken melanopsinasasleepmodulatorcircadiangatingofthedirecteffectsoflightonsleepandalteredsleephomeostasisinopn4mice AT patricebourgin melanopsinasasleepmodulatorcircadiangatingofthedirecteffectsoflightonsleepandalteredsleephomeostasisinopn4mice
_version_	1718414612823539712

Melanopsin as a sleep modulator: circadian gating of the direct effects of light on sleep and altered sleep homeostasis in Opn4(-/-) mice.

Ejemplares similares