Elevated levels of FMR1 mRNA in granulosa cells are associated with low ovarian reserve in FMR1 premutation carriers.

<h4>Aim</h4>To assess the role of mRNA accumulation in granulosa cells as the cause of low ovarian response among FMR1 premutation carriers undergoing pre-implantation genetic diagnosis (PGD).<h4>Design</h4>Case control study in an academic IVF unit. Twenty-one consecutive FM...

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Autores principales:	Shai E Elizur, Oshrit Lebovitz, Sanaz Derech-Haim, Olga Dratviman-Storobinsky, Baruch Feldman, Jehoshua Dor, Raoul Orvieto, Yoram Cohen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e99f8137474546bb9b17c9b491fa3399
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spelling	oai:doaj.org-article:e99f8137474546bb9b17c9b491fa33992021-11-25T06:03:26ZElevated levels of FMR1 mRNA in granulosa cells are associated with low ovarian reserve in FMR1 premutation carriers.1932-620310.1371/journal.pone.0105121https://doaj.org/article/e99f8137474546bb9b17c9b491fa33992014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25153074/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Aim</h4>To assess the role of mRNA accumulation in granulosa cells as the cause of low ovarian response among FMR1 premutation carriers undergoing pre-implantation genetic diagnosis (PGD).<h4>Design</h4>Case control study in an academic IVF unit. Twenty-one consecutive FMR1 premutation carriers and 15 control women were included. After oocyte retrieval the granulosa cells mRNA levels of FMR1 was measured using RT-PCR.<h4>Results</h4>In FMR1 premutation carriers, there was a significant non-linear association between the number of CGG repeats and the number of retrieved oocytes (p<0.0001) and a trend to granulosa cells FMR1 mRNA levels (p = 0.07). The lowest number of retrieved oocytes and the highest level of mRNA were seen in women with mid-size CGG repeats (80-120). A significant negative linear correlation was observed between the granulosa cells FMR1 mRNA levels and the number of retrieved oocytes (R2 linear = 0.231, P = 0.02).<h4>Conclusion</h4>We suggest that there is a no-linear association between the number of CGG repeats and ovarian function, resulting from an increased granulosa cells FMR1 mRNA accumulation in FMR1 carriers in the mid-range (80-120 repeats).Shai E ElizurOshrit LebovitzSanaz Derech-HaimOlga Dratviman-StorobinskyBaruch FeldmanJehoshua DorRaoul OrvietoYoram CohenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e105121 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shai E Elizur Oshrit Lebovitz Sanaz Derech-Haim Olga Dratviman-Storobinsky Baruch Feldman Jehoshua Dor Raoul Orvieto Yoram Cohen Elevated levels of FMR1 mRNA in granulosa cells are associated with low ovarian reserve in FMR1 premutation carriers.
description	<h4>Aim</h4>To assess the role of mRNA accumulation in granulosa cells as the cause of low ovarian response among FMR1 premutation carriers undergoing pre-implantation genetic diagnosis (PGD).<h4>Design</h4>Case control study in an academic IVF unit. Twenty-one consecutive FMR1 premutation carriers and 15 control women were included. After oocyte retrieval the granulosa cells mRNA levels of FMR1 was measured using RT-PCR.<h4>Results</h4>In FMR1 premutation carriers, there was a significant non-linear association between the number of CGG repeats and the number of retrieved oocytes (p<0.0001) and a trend to granulosa cells FMR1 mRNA levels (p = 0.07). The lowest number of retrieved oocytes and the highest level of mRNA were seen in women with mid-size CGG repeats (80-120). A significant negative linear correlation was observed between the granulosa cells FMR1 mRNA levels and the number of retrieved oocytes (R2 linear = 0.231, P = 0.02).<h4>Conclusion</h4>We suggest that there is a no-linear association between the number of CGG repeats and ovarian function, resulting from an increased granulosa cells FMR1 mRNA accumulation in FMR1 carriers in the mid-range (80-120 repeats).
format	article
author	Shai E Elizur Oshrit Lebovitz Sanaz Derech-Haim Olga Dratviman-Storobinsky Baruch Feldman Jehoshua Dor Raoul Orvieto Yoram Cohen
author_facet	Shai E Elizur Oshrit Lebovitz Sanaz Derech-Haim Olga Dratviman-Storobinsky Baruch Feldman Jehoshua Dor Raoul Orvieto Yoram Cohen
author_sort	Shai E Elizur
title	Elevated levels of FMR1 mRNA in granulosa cells are associated with low ovarian reserve in FMR1 premutation carriers.
title_short	Elevated levels of FMR1 mRNA in granulosa cells are associated with low ovarian reserve in FMR1 premutation carriers.
title_full	Elevated levels of FMR1 mRNA in granulosa cells are associated with low ovarian reserve in FMR1 premutation carriers.
title_fullStr	Elevated levels of FMR1 mRNA in granulosa cells are associated with low ovarian reserve in FMR1 premutation carriers.
title_full_unstemmed	Elevated levels of FMR1 mRNA in granulosa cells are associated with low ovarian reserve in FMR1 premutation carriers.
title_sort	elevated levels of fmr1 mrna in granulosa cells are associated with low ovarian reserve in fmr1 premutation carriers.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/e99f8137474546bb9b17c9b491fa3399
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Elevated levels of FMR1 mRNA in granulosa cells are associated with low ovarian reserve in FMR1 premutation carriers.

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