Modulation of the Gut Microbiota during High-Dose Glycerol Monolaurate-Mediated Amelioration of Obesity in Mice Fed a High-Fat Diet
ABSTRACT Obesity and associated metabolic disorders are worldwide public health issues. The gut microbiota plays a key role in the pathophysiology of diet-induced obesity. Glycerol monolaurate (GML) is a widely consumed food emulsifier with antibacterial properties. Here, we explore the anti-obesity...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
American Society for Microbiology
2020
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/e9a0229848f748d6bf43f1f049a3cc52 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:e9a0229848f748d6bf43f1f049a3cc52 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:e9a0229848f748d6bf43f1f049a3cc522021-11-15T15:57:02ZModulation of the Gut Microbiota during High-Dose Glycerol Monolaurate-Mediated Amelioration of Obesity in Mice Fed a High-Fat Diet10.1128/mBio.00190-202150-7511https://doaj.org/article/e9a0229848f748d6bf43f1f049a3cc522020-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00190-20https://doaj.org/toc/2150-7511ABSTRACT Obesity and associated metabolic disorders are worldwide public health issues. The gut microbiota plays a key role in the pathophysiology of diet-induced obesity. Glycerol monolaurate (GML) is a widely consumed food emulsifier with antibacterial properties. Here, we explore the anti-obesity effect of GML (1,600 mg/kg of body weight) in high-fat diet (HFD)-fed mice. HFD-fed mice were treated with 1,600 mg/kg GML. Integrated microbiome, metabolome, and transcriptome analyses were used to systematically investigate the metabolic effects of GML, and antibiotic treatment was used to assess the effects of GML on the gut microbiota. Our data indicated that GML significantly reduced body weight and visceral fat deposition, improved hyperlipidemia and hepatic lipid metabolism, and ameliorated glucose homeostasis and inflammation in HFD-fed mice. Importantly, GML modulated HFD-induced gut microbiota dysbiosis and selectively increased the abundance of Bifidobacterium pseudolongum. Antibiotic treatment abolished all the GML-mediated metabolic improvements. A multiomics (microbiome, metabolome, and transcriptome) association study showed that GML significantly modulated glycerophospholipid metabolism, and the abundance of Bifidobacterium pseudolongum strongly correlated with the metabolites and genes that participated in glycerophospholipid metabolism. Our results indicated that GML may be provided for obesity prevention by targeting the gut microbiota and regulating glycerophospholipid metabolism.Minjie ZhaoZengliang JiangHaiying CaiYang LiQiufen MoLingli DengHao ZhongTao LiuHui ZhangJing X. KangFengqin FengAmerican Society for Microbiologyarticleglycerol monolaurateobesitygut microbiotaserum metabolomehepatic transcriptomeMicrobiologyQR1-502ENmBio, Vol 11, Iss 2 (2020) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
glycerol monolaurate obesity gut microbiota serum metabolome hepatic transcriptome Microbiology QR1-502 |
| spellingShingle |
glycerol monolaurate obesity gut microbiota serum metabolome hepatic transcriptome Microbiology QR1-502 Minjie Zhao Zengliang Jiang Haiying Cai Yang Li Qiufen Mo Lingli Deng Hao Zhong Tao Liu Hui Zhang Jing X. Kang Fengqin Feng Modulation of the Gut Microbiota during High-Dose Glycerol Monolaurate-Mediated Amelioration of Obesity in Mice Fed a High-Fat Diet |
| description |
ABSTRACT Obesity and associated metabolic disorders are worldwide public health issues. The gut microbiota plays a key role in the pathophysiology of diet-induced obesity. Glycerol monolaurate (GML) is a widely consumed food emulsifier with antibacterial properties. Here, we explore the anti-obesity effect of GML (1,600 mg/kg of body weight) in high-fat diet (HFD)-fed mice. HFD-fed mice were treated with 1,600 mg/kg GML. Integrated microbiome, metabolome, and transcriptome analyses were used to systematically investigate the metabolic effects of GML, and antibiotic treatment was used to assess the effects of GML on the gut microbiota. Our data indicated that GML significantly reduced body weight and visceral fat deposition, improved hyperlipidemia and hepatic lipid metabolism, and ameliorated glucose homeostasis and inflammation in HFD-fed mice. Importantly, GML modulated HFD-induced gut microbiota dysbiosis and selectively increased the abundance of Bifidobacterium pseudolongum. Antibiotic treatment abolished all the GML-mediated metabolic improvements. A multiomics (microbiome, metabolome, and transcriptome) association study showed that GML significantly modulated glycerophospholipid metabolism, and the abundance of Bifidobacterium pseudolongum strongly correlated with the metabolites and genes that participated in glycerophospholipid metabolism. Our results indicated that GML may be provided for obesity prevention by targeting the gut microbiota and regulating glycerophospholipid metabolism. |
| format |
article |
| author |
Minjie Zhao Zengliang Jiang Haiying Cai Yang Li Qiufen Mo Lingli Deng Hao Zhong Tao Liu Hui Zhang Jing X. Kang Fengqin Feng |
| author_facet |
Minjie Zhao Zengliang Jiang Haiying Cai Yang Li Qiufen Mo Lingli Deng Hao Zhong Tao Liu Hui Zhang Jing X. Kang Fengqin Feng |
| author_sort |
Minjie Zhao |
| title |
Modulation of the Gut Microbiota during High-Dose Glycerol Monolaurate-Mediated Amelioration of Obesity in Mice Fed a High-Fat Diet |
| title_short |
Modulation of the Gut Microbiota during High-Dose Glycerol Monolaurate-Mediated Amelioration of Obesity in Mice Fed a High-Fat Diet |
| title_full |
Modulation of the Gut Microbiota during High-Dose Glycerol Monolaurate-Mediated Amelioration of Obesity in Mice Fed a High-Fat Diet |
| title_fullStr |
Modulation of the Gut Microbiota during High-Dose Glycerol Monolaurate-Mediated Amelioration of Obesity in Mice Fed a High-Fat Diet |
| title_full_unstemmed |
Modulation of the Gut Microbiota during High-Dose Glycerol Monolaurate-Mediated Amelioration of Obesity in Mice Fed a High-Fat Diet |
| title_sort |
modulation of the gut microbiota during high-dose glycerol monolaurate-mediated amelioration of obesity in mice fed a high-fat diet |
| publisher |
American Society for Microbiology |
| publishDate |
2020 |
| url |
https://doaj.org/article/e9a0229848f748d6bf43f1f049a3cc52 |
| work_keys_str_mv |
AT minjiezhao modulationofthegutmicrobiotaduringhighdoseglycerolmonolauratemediatedameliorationofobesityinmicefedahighfatdiet AT zengliangjiang modulationofthegutmicrobiotaduringhighdoseglycerolmonolauratemediatedameliorationofobesityinmicefedahighfatdiet AT haiyingcai modulationofthegutmicrobiotaduringhighdoseglycerolmonolauratemediatedameliorationofobesityinmicefedahighfatdiet AT yangli modulationofthegutmicrobiotaduringhighdoseglycerolmonolauratemediatedameliorationofobesityinmicefedahighfatdiet AT qiufenmo modulationofthegutmicrobiotaduringhighdoseglycerolmonolauratemediatedameliorationofobesityinmicefedahighfatdiet AT linglideng modulationofthegutmicrobiotaduringhighdoseglycerolmonolauratemediatedameliorationofobesityinmicefedahighfatdiet AT haozhong modulationofthegutmicrobiotaduringhighdoseglycerolmonolauratemediatedameliorationofobesityinmicefedahighfatdiet AT taoliu modulationofthegutmicrobiotaduringhighdoseglycerolmonolauratemediatedameliorationofobesityinmicefedahighfatdiet AT huizhang modulationofthegutmicrobiotaduringhighdoseglycerolmonolauratemediatedameliorationofobesityinmicefedahighfatdiet AT jingxkang modulationofthegutmicrobiotaduringhighdoseglycerolmonolauratemediatedameliorationofobesityinmicefedahighfatdiet AT fengqinfeng modulationofthegutmicrobiotaduringhighdoseglycerolmonolauratemediatedameliorationofobesityinmicefedahighfatdiet |
| _version_ |
1718427062570582016 |