Raw Glycerol Based Medium for DHA and Lipids Production, Using the Marine Heterotrophic Microalga <i>Crypthecodinium cohnii</i>

Crude glycerol, a biodiesel industry byproduct, and corn steep liquor (CSL) derived from a starch industry, were used as carbon and nitrogen sources, respectively, for lipid production, using the heterotrophic microalga <i>C. cohnii</i> grown in a bench bioreactor, in a batch culture. Th...

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Autores principales:	Patrícia Moniz, Carla Silva, Ana Cristina Oliveira, Alberto Reis, Teresa Lopes da Silva
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Crypthecodinium cohnii</i> cultivation alternative carbon source alternative nitrogen source flow cytometry potential costs impact in upscaled systems Chemical technology TP1-1185 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/e9a14562ed174fb5afc31525c90a855f
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spelling	oai:doaj.org-article:e9a14562ed174fb5afc31525c90a855f2021-11-25T18:51:24ZRaw Glycerol Based Medium for DHA and Lipids Production, Using the Marine Heterotrophic Microalga <i>Crypthecodinium cohnii</i>10.3390/pr91120052227-9717https://doaj.org/article/e9a14562ed174fb5afc31525c90a855f2021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9717/9/11/2005https://doaj.org/toc/2227-9717Crude glycerol, a biodiesel industry byproduct, and corn steep liquor (CSL) derived from a starch industry, were used as carbon and nitrogen sources, respectively, for lipid production, using the heterotrophic microalga <i>C. cohnii</i> grown in a bench bioreactor, in a batch culture. The maximum biomass concentration, lipid content and lipid productivity attained were 5.34 g/L, 24.6% (<i>w</i>/<i>w</i> Dry Cell Weight-DCW) and 0.016 g L<sup>−1</sup> h<sup>−1</sup>, respectively. Flow cytometry analysis was used to evaluate the impact of these substrates on the microalgae cells. A high proportion of intact cells with enzymatic (esterases) activity (>50%) was present throughout the cultivation time course. These results indicate that crude glycerol and CSL can be used in the medium formulation for DHA and lipid production using this microalga, which reduce the process costs in an expected maximum of 84%.Patrícia MonizCarla SilvaAna Cristina OliveiraAlberto ReisTeresa Lopes da SilvaMDPI AGarticle<i>Crypthecodinium cohnii</i>cultivationalternative carbon sourcealternative nitrogen sourceflow cytometrypotential costs impact in upscaled systemsChemical technologyTP1-1185ChemistryQD1-999ENProcesses, Vol 9, Iss 2005, p 2005 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Crypthecodinium cohnii</i> cultivation alternative carbon source alternative nitrogen source flow cytometry potential costs impact in upscaled systems Chemical technology TP1-1185 Chemistry QD1-999
spellingShingle	<i>Crypthecodinium cohnii</i> cultivation alternative carbon source alternative nitrogen source flow cytometry potential costs impact in upscaled systems Chemical technology TP1-1185 Chemistry QD1-999 Patrícia Moniz Carla Silva Ana Cristina Oliveira Alberto Reis Teresa Lopes da Silva Raw Glycerol Based Medium for DHA and Lipids Production, Using the Marine Heterotrophic Microalga <i>Crypthecodinium cohnii</i>
description	Crude glycerol, a biodiesel industry byproduct, and corn steep liquor (CSL) derived from a starch industry, were used as carbon and nitrogen sources, respectively, for lipid production, using the heterotrophic microalga <i>C. cohnii</i> grown in a bench bioreactor, in a batch culture. The maximum biomass concentration, lipid content and lipid productivity attained were 5.34 g/L, 24.6% (<i>w</i>/<i>w</i> Dry Cell Weight-DCW) and 0.016 g L<sup>−1</sup> h<sup>−1</sup>, respectively. Flow cytometry analysis was used to evaluate the impact of these substrates on the microalgae cells. A high proportion of intact cells with enzymatic (esterases) activity (>50%) was present throughout the cultivation time course. These results indicate that crude glycerol and CSL can be used in the medium formulation for DHA and lipid production using this microalga, which reduce the process costs in an expected maximum of 84%.
format	article
author	Patrícia Moniz Carla Silva Ana Cristina Oliveira Alberto Reis Teresa Lopes da Silva
author_facet	Patrícia Moniz Carla Silva Ana Cristina Oliveira Alberto Reis Teresa Lopes da Silva
author_sort	Patrícia Moniz
title	Raw Glycerol Based Medium for DHA and Lipids Production, Using the Marine Heterotrophic Microalga <i>Crypthecodinium cohnii</i>
title_short	Raw Glycerol Based Medium for DHA and Lipids Production, Using the Marine Heterotrophic Microalga <i>Crypthecodinium cohnii</i>
title_full	Raw Glycerol Based Medium for DHA and Lipids Production, Using the Marine Heterotrophic Microalga <i>Crypthecodinium cohnii</i>
title_fullStr	Raw Glycerol Based Medium for DHA and Lipids Production, Using the Marine Heterotrophic Microalga <i>Crypthecodinium cohnii</i>
title_full_unstemmed	Raw Glycerol Based Medium for DHA and Lipids Production, Using the Marine Heterotrophic Microalga <i>Crypthecodinium cohnii</i>
title_sort	raw glycerol based medium for dha and lipids production, using the marine heterotrophic microalga <i>crypthecodinium cohnii</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e9a14562ed174fb5afc31525c90a855f
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Raw Glycerol Based Medium for DHA and Lipids Production, Using the Marine Heterotrophic Microalga <i>Crypthecodinium cohnii</i>

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