THE ROLE OF mutR GENE IN METABOLISM AND VIRULENCE OF emm12 GENOTYPE STREPTOCOCCUS PYOGENES STRAINS

In the present study the functional role of the mutR regulatory protein gene of Streptococcus pyogenes (emm12) was studied. The mutR gene was inactivated in the strains no. 97 and no. 152 by insertional mutagenesis. Inactivation of the mutR gene was found to affect the dynamic and characteristics of...

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Autores principales:	A. A. Zutkis, B. L. Milman, A. V. Dmitriev
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2015
Materias:	streptococcus pyogenes mutr transcriptional regulation metabolism virulence. Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/e9a44a2a02954c36afcf0838d8ce8361
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spelling	oai:doaj.org-article:e9a44a2a02954c36afcf0838d8ce83612021-11-22T07:09:47ZTHE ROLE OF mutR GENE IN METABOLISM AND VIRULENCE OF emm12 GENOTYPE STREPTOCOCCUS PYOGENES STRAINS2220-76192313-739810.15789/2220-7619-2014-4-339-346https://doaj.org/article/e9a44a2a02954c36afcf0838d8ce83612015-02-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/244https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398In the present study the functional role of the mutR regulatory protein gene of Streptococcus pyogenes (emm12) was studied. The mutR gene was inactivated in the strains no. 97 and no. 152 by insertional mutagenesis. Inactivation of the mutR gene was found to affect the dynamic and characteristics of bacterial growth in liquid medium. Expression of secreted nucleases was significantly lower in the mutant strains compared to the wild-type strains. Two-dimensional electrophoresis and mass-spectrometry revealed differences in expression of number of the proteins in mutant strainscompared to the wild-type strains. Inactivation of the mutR gene negatively affected capacity of S. pyogenes to adhere to human epithelial cells. Finally, the virulence properties of the no. 152[mutR] mutant strains were found to be 4,7-fold less compared to the strain no. 152, while the no. 97[mutR] mutant strain became avirulent compared to the strain no. 97 due to insertional inactivation of the mutR gene.A. A. ZutkisB. L. MilmanA. V. DmitrievSankt-Peterburg : NIIÈM imeni Pasteraarticlestreptococcus pyogenesmutrtranscriptional regulationmetabolismvirulence.Infectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 4, Iss 4, Pp 339-346 (2015)
institution	DOAJ
collection	DOAJ
language	RU
topic	streptococcus pyogenes mutr transcriptional regulation metabolism virulence. Infectious and parasitic diseases RC109-216
spellingShingle	streptococcus pyogenes mutr transcriptional regulation metabolism virulence. Infectious and parasitic diseases RC109-216 A. A. Zutkis B. L. Milman A. V. Dmitriev THE ROLE OF mutR GENE IN METABOLISM AND VIRULENCE OF emm12 GENOTYPE STREPTOCOCCUS PYOGENES STRAINS
description	In the present study the functional role of the mutR regulatory protein gene of Streptococcus pyogenes (emm12) was studied. The mutR gene was inactivated in the strains no. 97 and no. 152 by insertional mutagenesis. Inactivation of the mutR gene was found to affect the dynamic and characteristics of bacterial growth in liquid medium. Expression of secreted nucleases was significantly lower in the mutant strains compared to the wild-type strains. Two-dimensional electrophoresis and mass-spectrometry revealed differences in expression of number of the proteins in mutant strainscompared to the wild-type strains. Inactivation of the mutR gene negatively affected capacity of S. pyogenes to adhere to human epithelial cells. Finally, the virulence properties of the no. 152[mutR] mutant strains were found to be 4,7-fold less compared to the strain no. 152, while the no. 97[mutR] mutant strain became avirulent compared to the strain no. 97 due to insertional inactivation of the mutR gene.
format	article
author	A. A. Zutkis B. L. Milman A. V. Dmitriev
author_facet	A. A. Zutkis B. L. Milman A. V. Dmitriev
author_sort	A. A. Zutkis
title	THE ROLE OF mutR GENE IN METABOLISM AND VIRULENCE OF emm12 GENOTYPE STREPTOCOCCUS PYOGENES STRAINS
title_short	THE ROLE OF mutR GENE IN METABOLISM AND VIRULENCE OF emm12 GENOTYPE STREPTOCOCCUS PYOGENES STRAINS
title_full	THE ROLE OF mutR GENE IN METABOLISM AND VIRULENCE OF emm12 GENOTYPE STREPTOCOCCUS PYOGENES STRAINS
title_fullStr	THE ROLE OF mutR GENE IN METABOLISM AND VIRULENCE OF emm12 GENOTYPE STREPTOCOCCUS PYOGENES STRAINS
title_full_unstemmed	THE ROLE OF mutR GENE IN METABOLISM AND VIRULENCE OF emm12 GENOTYPE STREPTOCOCCUS PYOGENES STRAINS
title_sort	role of mutr gene in metabolism and virulence of emm12 genotype streptococcus pyogenes strains
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2015
url	https://doaj.org/article/e9a44a2a02954c36afcf0838d8ce8361
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THE ROLE OF mutR GENE IN METABOLISM AND VIRULENCE OF emm12 GENOTYPE STREPTOCOCCUS PYOGENES STRAINS

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