Coxiella burnetii replicates in Galleria mellonella hemocytes and transcriptome mapping reveals in vivo regulated genes

Larvae of the greater wax moth (Galleria mellonella) are susceptible to infection with C. burnetii, an obligate intracellular bacterial pathogen. We show that bacteria are found in hemocytes after infection, and occupy vacuoles which are morphologically similar to Coxiella-containing vacuoles seen i...

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Autores principales:	Andrea Kovacs-Simon, Georgie Metters, Isobel Norville, Claudia Hemsley, Richard W. Titball
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	coxiella burnetii galleria mellonella t4ss virulence transcriptome profiling Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/e9b36c16543f49f9bae62693b1a3b14e
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spelling	oai:doaj.org-article:e9b36c16543f49f9bae62693b1a3b14e2021-11-17T14:21:59ZCoxiella burnetii replicates in Galleria mellonella hemocytes and transcriptome mapping reveals in vivo regulated genes2150-55942150-560810.1080/21505594.2020.1819111https://doaj.org/article/e9b36c16543f49f9bae62693b1a3b14e2020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1819111https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Larvae of the greater wax moth (Galleria mellonella) are susceptible to infection with C. burnetii, an obligate intracellular bacterial pathogen. We show that bacteria are found in hemocytes after infection, and occupy vacuoles which are morphologically similar to Coxiella-containing vacuoles seen in infected mammalian phagocytes. We characterized the infection by transcriptome profiling of bacteria isolated from the hemocytes of infected larvae and identified 46 highly upregulated genes. The encoded proteins are predicted to be involved in translation, LPS biosynthesis, biotin synthesis, scavenging of reactive oxygen species, and included a T4SS effector and 30 hypothetical proteins. Some of these genes had previously been shown to be upregulated in buffalo green monkey (BGM) cells or in mice, whilst others appear to be regulated in a host-specific manner. Altogether, our results demonstrate the value of the G. mellonella model to study intracellular growth and identify potential virulence factors of C. burnetii.Andrea Kovacs-SimonGeorgie MettersIsobel NorvilleClaudia HemsleyRichard W. TitballTaylor & Francis Grouparticlecoxiella burnetiigalleria mellonellat4ssvirulencetranscriptome profilingInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 1268-1278 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	coxiella burnetii galleria mellonella t4ss virulence transcriptome profiling Infectious and parasitic diseases RC109-216
spellingShingle	coxiella burnetii galleria mellonella t4ss virulence transcriptome profiling Infectious and parasitic diseases RC109-216 Andrea Kovacs-Simon Georgie Metters Isobel Norville Claudia Hemsley Richard W. Titball Coxiella burnetii replicates in Galleria mellonella hemocytes and transcriptome mapping reveals in vivo regulated genes
description	Larvae of the greater wax moth (Galleria mellonella) are susceptible to infection with C. burnetii, an obligate intracellular bacterial pathogen. We show that bacteria are found in hemocytes after infection, and occupy vacuoles which are morphologically similar to Coxiella-containing vacuoles seen in infected mammalian phagocytes. We characterized the infection by transcriptome profiling of bacteria isolated from the hemocytes of infected larvae and identified 46 highly upregulated genes. The encoded proteins are predicted to be involved in translation, LPS biosynthesis, biotin synthesis, scavenging of reactive oxygen species, and included a T4SS effector and 30 hypothetical proteins. Some of these genes had previously been shown to be upregulated in buffalo green monkey (BGM) cells or in mice, whilst others appear to be regulated in a host-specific manner. Altogether, our results demonstrate the value of the G. mellonella model to study intracellular growth and identify potential virulence factors of C. burnetii.
format	article
author	Andrea Kovacs-Simon Georgie Metters Isobel Norville Claudia Hemsley Richard W. Titball
author_facet	Andrea Kovacs-Simon Georgie Metters Isobel Norville Claudia Hemsley Richard W. Titball
author_sort	Andrea Kovacs-Simon
title	Coxiella burnetii replicates in Galleria mellonella hemocytes and transcriptome mapping reveals in vivo regulated genes
title_short	Coxiella burnetii replicates in Galleria mellonella hemocytes and transcriptome mapping reveals in vivo regulated genes
title_full	Coxiella burnetii replicates in Galleria mellonella hemocytes and transcriptome mapping reveals in vivo regulated genes
title_fullStr	Coxiella burnetii replicates in Galleria mellonella hemocytes and transcriptome mapping reveals in vivo regulated genes
title_full_unstemmed	Coxiella burnetii replicates in Galleria mellonella hemocytes and transcriptome mapping reveals in vivo regulated genes
title_sort	coxiella burnetii replicates in galleria mellonella hemocytes and transcriptome mapping reveals in vivo regulated genes
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/e9b36c16543f49f9bae62693b1a3b14e
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Coxiella burnetii replicates in Galleria mellonella hemocytes and transcriptome mapping reveals in vivo regulated genes

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