Insecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan

Abstract Insecticide resistant Aedes populations have recently been reported in Pakistan, imposing a threat to their control. We aimed to evaluate the susceptibility of Aedes aegypti and Aedes albopictus populations from Lahore to WHO-recommended insecticides and to investigate metabolic and target-...

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Autores principales:	Rafi Ur Rahman, Barbara Souza, Iftikhar Uddin, Luana Carrara, Luiz Paulo Brito, Monique Melo Costa, Muhammad Asif Mahmood, Sozaina Khan, Jose Bento Pereira Lima, Ademir Jesus Martins
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e9c13e905bda4bd59b16e340fa8f1f6d
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spelling	oai:doaj.org-article:e9c13e905bda4bd59b16e340fa8f1f6d2021-12-02T13:33:45ZInsecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan10.1038/s41598-021-83465-w2045-2322https://doaj.org/article/e9c13e905bda4bd59b16e340fa8f1f6d2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83465-whttps://doaj.org/toc/2045-2322Abstract Insecticide resistant Aedes populations have recently been reported in Pakistan, imposing a threat to their control. We aimed to evaluate the susceptibility of Aedes aegypti and Aedes albopictus populations from Lahore to WHO-recommended insecticides and to investigate metabolic and target-site resistance mechanisms. For this purpose, we first carried out bioassays with the larvicides temephos and pyriproxyfen, and the adulticides malathion, permethrin, deltamethrin, alpha-cypermethrin, and etofenprox. We looked for Knockdown resistance mutations (kdr) by qPCR, High-Resolution Melt (HRM), and sequencing. In order to explore the role of detoxifying enzymes in resistance, we carried out synergist bioassay with both species and then checked the expression of CYP9M6, CYP9J10, CYP9J28, CYP6BB2, CCAe3a, and SAP2 genes in Ae. aegypti. Both species were susceptible to organophosphates and the insect growth regulator, however resistant to all pyrethroids. We are reporting the kdr haplotypes 1520Ile + 1534Cys and T1520 + 1534Cys in high frequencies in Ae. aegypti while Ae. albopictus only exhibited the alteration L882M. PBO increased the sensitivity to permethrin in Ae. aegypti, suggesting the participation of P450 genes in conferring resistance, and indeed, CYP928 was highly expressed. We presume that dengue vectors in Lahore city are resistant to pyrethroids, probably due to multiple mechanisms, such as kdr mutations and P450 overexpression.Rafi Ur RahmanBarbara SouzaIftikhar UddinLuana CarraraLuiz Paulo BritoMonique Melo CostaMuhammad Asif MahmoodSozaina KhanJose Bento Pereira LimaAdemir Jesus MartinsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rafi Ur Rahman Barbara Souza Iftikhar Uddin Luana Carrara Luiz Paulo Brito Monique Melo Costa Muhammad Asif Mahmood Sozaina Khan Jose Bento Pereira Lima Ademir Jesus Martins Insecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan
description	Abstract Insecticide resistant Aedes populations have recently been reported in Pakistan, imposing a threat to their control. We aimed to evaluate the susceptibility of Aedes aegypti and Aedes albopictus populations from Lahore to WHO-recommended insecticides and to investigate metabolic and target-site resistance mechanisms. For this purpose, we first carried out bioassays with the larvicides temephos and pyriproxyfen, and the adulticides malathion, permethrin, deltamethrin, alpha-cypermethrin, and etofenprox. We looked for Knockdown resistance mutations (kdr) by qPCR, High-Resolution Melt (HRM), and sequencing. In order to explore the role of detoxifying enzymes in resistance, we carried out synergist bioassay with both species and then checked the expression of CYP9M6, CYP9J10, CYP9J28, CYP6BB2, CCAe3a, and SAP2 genes in Ae. aegypti. Both species were susceptible to organophosphates and the insect growth regulator, however resistant to all pyrethroids. We are reporting the kdr haplotypes 1520Ile + 1534Cys and T1520 + 1534Cys in high frequencies in Ae. aegypti while Ae. albopictus only exhibited the alteration L882M. PBO increased the sensitivity to permethrin in Ae. aegypti, suggesting the participation of P450 genes in conferring resistance, and indeed, CYP928 was highly expressed. We presume that dengue vectors in Lahore city are resistant to pyrethroids, probably due to multiple mechanisms, such as kdr mutations and P450 overexpression.
format	article
author	Rafi Ur Rahman Barbara Souza Iftikhar Uddin Luana Carrara Luiz Paulo Brito Monique Melo Costa Muhammad Asif Mahmood Sozaina Khan Jose Bento Pereira Lima Ademir Jesus Martins
author_facet	Rafi Ur Rahman Barbara Souza Iftikhar Uddin Luana Carrara Luiz Paulo Brito Monique Melo Costa Muhammad Asif Mahmood Sozaina Khan Jose Bento Pereira Lima Ademir Jesus Martins
author_sort	Rafi Ur Rahman
title	Insecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan
title_short	Insecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan
title_full	Insecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan
title_fullStr	Insecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan
title_full_unstemmed	Insecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan
title_sort	insecticide resistance and underlying targets-site and metabolic mechanisms in aedes aegypti and aedes albopictus from lahore, pakistan
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e9c13e905bda4bd59b16e340fa8f1f6d
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Insecticide resistance and underlying targets-site and metabolic mechanisms in Aedes aegypti and Aedes albopictus from Lahore, Pakistan

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