Serotonin 2A (5-HT2A) receptor affects cell–matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells

Abstract 5-HT2A, a G-protein coupled receptor, is widely expressed in the human body, including in the gastrointestinal tract, platelets and the nervous system. It mediates various functions, for e.g. learning, memory, mood regulation, platelet aggregation and vasoconstriction, but its involvement i...

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Autores principales:	Joe Anand Kumar John Jayakumar, Mitradas. M. Panicker, Basudha Basu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e9cdffe2a8414cb29a3524b3fb617787
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spelling	oai:doaj.org-article:e9cdffe2a8414cb29a3524b3fb6177872021-12-02T12:33:05ZSerotonin 2A (5-HT2A) receptor affects cell–matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells10.1038/s41598-020-78595-62045-2322https://doaj.org/article/e9cdffe2a8414cb29a3524b3fb6177872020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78595-6https://doaj.org/toc/2045-2322Abstract 5-HT2A, a G-protein coupled receptor, is widely expressed in the human body, including in the gastrointestinal tract, platelets and the nervous system. It mediates various functions, for e.g. learning, memory, mood regulation, platelet aggregation and vasoconstriction, but its involvement in cell-adhesion remains largely unknown. Here we report a novel role for 5-HT2A in cell–matrix adhesion. In HEK293 cells, which are loosely adherent, expression and stimulation of human or rat 5-HT2A receptor by agonists such as serotonin or 2,5-dimethoxy-4-iodoamphetamine (DOI) led to a significant increase in adhesion, while inhibition of 5-HT2A by antipsychotics, such as risperidone, olanzapine or chlorpromazine prevented it. 5-HT2A activation gave rise to stress fibers in these cells and was also required for their maintenance. Mechanistically, the 5-HT2A-mediated adhesion was mediated by downstream PKC and Rho signaling. Since 5-HT2A is associated with many disorders such as dementia, depression and schizophrenia, its role in cell–matrix adhesion could have implications for neural circuits.Joe Anand Kumar John JayakumarMitradas. M. PanickerBasudha BasuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joe Anand Kumar John Jayakumar Mitradas. M. Panicker Basudha Basu Serotonin 2A (5-HT2A) receptor affects cell–matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells
description	Abstract 5-HT2A, a G-protein coupled receptor, is widely expressed in the human body, including in the gastrointestinal tract, platelets and the nervous system. It mediates various functions, for e.g. learning, memory, mood regulation, platelet aggregation and vasoconstriction, but its involvement in cell-adhesion remains largely unknown. Here we report a novel role for 5-HT2A in cell–matrix adhesion. In HEK293 cells, which are loosely adherent, expression and stimulation of human or rat 5-HT2A receptor by agonists such as serotonin or 2,5-dimethoxy-4-iodoamphetamine (DOI) led to a significant increase in adhesion, while inhibition of 5-HT2A by antipsychotics, such as risperidone, olanzapine or chlorpromazine prevented it. 5-HT2A activation gave rise to stress fibers in these cells and was also required for their maintenance. Mechanistically, the 5-HT2A-mediated adhesion was mediated by downstream PKC and Rho signaling. Since 5-HT2A is associated with many disorders such as dementia, depression and schizophrenia, its role in cell–matrix adhesion could have implications for neural circuits.
format	article
author	Joe Anand Kumar John Jayakumar Mitradas. M. Panicker Basudha Basu
author_facet	Joe Anand Kumar John Jayakumar Mitradas. M. Panicker Basudha Basu
author_sort	Joe Anand Kumar John Jayakumar
title	Serotonin 2A (5-HT2A) receptor affects cell–matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells
title_short	Serotonin 2A (5-HT2A) receptor affects cell–matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells
title_full	Serotonin 2A (5-HT2A) receptor affects cell–matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells
title_fullStr	Serotonin 2A (5-HT2A) receptor affects cell–matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells
title_full_unstemmed	Serotonin 2A (5-HT2A) receptor affects cell–matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells
title_sort	serotonin 2a (5-ht2a) receptor affects cell–matrix adhesion and the formation and maintenance of stress fibers in hek293 cells
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/e9cdffe2a8414cb29a3524b3fb617787
work_keys_str_mv	AT joeanandkumarjohnjayakumar serotonin2a5ht2areceptoraffectscellmatrixadhesionandtheformationandmaintenanceofstressfibersinhek293cells AT mitradasmpanicker serotonin2a5ht2areceptoraffectscellmatrixadhesionandtheformationandmaintenanceofstressfibersinhek293cells AT basudhabasu serotonin2a5ht2areceptoraffectscellmatrixadhesionandtheformationandmaintenanceofstressfibersinhek293cells
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Serotonin 2A (5-HT2A) receptor affects cell–matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells

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