Antioxidative nanofullerol inhibits macrophage activation and development of osteoarthritis in rats

Yilun Pei,1,* Fuai Cui,1,2,* Xuejun Du,1 Guowei Shang,1 Wanan Xiao,1 Xinlin Yang,1 Quanjun Cui11Orthopaedic Research Lab, University of Virginia, Charlottesville, VA, USA; 2Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Shandong University, Jinan, Shandong, China...

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Autores principales:	Pei Y, Cui F, Du X, Shang G, Xiao W, Yang X, Cui Q
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Macrophages inflammation osteoarthritis antioxidant polyhydroxylated fullerene C60 Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e9ce0428752c4f97aaab77177cd6ef30
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spelling	oai:doaj.org-article:e9ce0428752c4f97aaab77177cd6ef302021-12-02T05:16:29ZAntioxidative nanofullerol inhibits macrophage activation and development of osteoarthritis in rats1178-2013https://doaj.org/article/e9ce0428752c4f97aaab77177cd6ef302019-06-01T00:00:00Zhttps://www.dovepress.com/antioxidative-nanofullerol-inhibits-macrophage-activation-and-developm-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yilun Pei,1,* Fuai Cui,1,2,* Xuejun Du,1 Guowei Shang,1 Wanan Xiao,1 Xinlin Yang,1 Quanjun Cui11Orthopaedic Research Lab, University of Virginia, Charlottesville, VA, USA; 2Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Shandong University, Jinan, Shandong, China*These authors contributed equally to this work Background: There is emerging evidence which suggests that cellular ROS including nitric oxide (NO) are important mediators for inflammation and osteoarthritis (OA). Water-soluble polyhydroxylated fullerene C60 (fullerol) nanoparticle has been demonstrated to have an outstanding ability to scavenge ROS. Purpose: The objective of this study is to assess the effects of fullerol on inflammation and OA by in vitro and in vivo studies.Methods: For in vitro experiments, primary mouse peritoneal macrophages and a macrophage cell line RAW264.7 were stimulated to inflammatory phenotypes by lipopolysaccharide (LPS) in the presence of fullerol. For the animal study, OA model was created by intra-articular injection of monoiodoacetate into the knee joints of rats and fullerol was intravenously injected immediately after OA induction.Results: NO production and pro-inflammatory gene expression induced by LPS was significantly diminished by fullerol in both macrophage cell types. Meanwhile, fullerol could remarkably reduce phosphorylation of p38 mitogen-activated protein kinase, and protein level of transcription factors nuclear factor-kappaB and forkhead box transcription factor 1 within the nucleus. The animal study delineated that systematic administration of fullerol prevented OA, inhibiting inflammation of synovial membranes and the damage toward the cartilage chondrocytes in the OA joints.Conclusion: Antioxidative fullerol may have a potential therapeutic application for OA.Keywords: macrophages, inflammation, osteoarthritis, antioxidant, polyhydroxylated fullerene C60Pei YCui FDu XShang GXiao WYang XCui QDove Medical PressarticleMacrophagesinflammationosteoarthritisantioxidantpolyhydroxylated fullerene C60Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 4145-4155 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Macrophages inflammation osteoarthritis antioxidant polyhydroxylated fullerene C60 Medicine (General) R5-920
spellingShingle	Macrophages inflammation osteoarthritis antioxidant polyhydroxylated fullerene C60 Medicine (General) R5-920 Pei Y Cui F Du X Shang G Xiao W Yang X Cui Q Antioxidative nanofullerol inhibits macrophage activation and development of osteoarthritis in rats
description	Yilun Pei,1,* Fuai Cui,1,2,* Xuejun Du,1 Guowei Shang,1 Wanan Xiao,1 Xinlin Yang,1 Quanjun Cui11Orthopaedic Research Lab, University of Virginia, Charlottesville, VA, USA; 2Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Shandong University, Jinan, Shandong, China*These authors contributed equally to this work Background: There is emerging evidence which suggests that cellular ROS including nitric oxide (NO) are important mediators for inflammation and osteoarthritis (OA). Water-soluble polyhydroxylated fullerene C60 (fullerol) nanoparticle has been demonstrated to have an outstanding ability to scavenge ROS. Purpose: The objective of this study is to assess the effects of fullerol on inflammation and OA by in vitro and in vivo studies.Methods: For in vitro experiments, primary mouse peritoneal macrophages and a macrophage cell line RAW264.7 were stimulated to inflammatory phenotypes by lipopolysaccharide (LPS) in the presence of fullerol. For the animal study, OA model was created by intra-articular injection of monoiodoacetate into the knee joints of rats and fullerol was intravenously injected immediately after OA induction.Results: NO production and pro-inflammatory gene expression induced by LPS was significantly diminished by fullerol in both macrophage cell types. Meanwhile, fullerol could remarkably reduce phosphorylation of p38 mitogen-activated protein kinase, and protein level of transcription factors nuclear factor-kappaB and forkhead box transcription factor 1 within the nucleus. The animal study delineated that systematic administration of fullerol prevented OA, inhibiting inflammation of synovial membranes and the damage toward the cartilage chondrocytes in the OA joints.Conclusion: Antioxidative fullerol may have a potential therapeutic application for OA.Keywords: macrophages, inflammation, osteoarthritis, antioxidant, polyhydroxylated fullerene C60
format	article
author	Pei Y Cui F Du X Shang G Xiao W Yang X Cui Q
author_facet	Pei Y Cui F Du X Shang G Xiao W Yang X Cui Q
author_sort	Pei Y
title	Antioxidative nanofullerol inhibits macrophage activation and development of osteoarthritis in rats
title_short	Antioxidative nanofullerol inhibits macrophage activation and development of osteoarthritis in rats
title_full	Antioxidative nanofullerol inhibits macrophage activation and development of osteoarthritis in rats
title_fullStr	Antioxidative nanofullerol inhibits macrophage activation and development of osteoarthritis in rats
title_full_unstemmed	Antioxidative nanofullerol inhibits macrophage activation and development of osteoarthritis in rats
title_sort	antioxidative nanofullerol inhibits macrophage activation and development of osteoarthritis in rats
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/e9ce0428752c4f97aaab77177cd6ef30
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Antioxidative nanofullerol inhibits macrophage activation and development of osteoarthritis in rats

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